scholarly journals Induced Endothelial Cells Transdifferentiated from Fibroblasts Derived from Neonates and Patients with Peripheral Arterial Disease Demonstrate Comparable Angiogenic Properties

2016 ◽  
Vol 25 ◽  
pp. S1
Author(s):  
J. Hywood ◽  
S. Sadeghipour ◽  
Z. Clayton ◽  
J. Yuan ◽  
J. Wong ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial

scholarly journals Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255075
Author(s):  
Jack D. Hywood ◽  
Sara Sadeghipour ◽  
Zoe E. Clayton ◽  
Jun Yuan ◽  
Colleen Stubbs ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Peripheral Arterial Disease ◽  
Murine Model ◽  
Arterial Disease ◽  
Scid Mice ◽  
Laser Doppler ◽  
Cytokine Secretion ◽  
Endothelial Phenotype ◽  
Peripheral Arterial

Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and whether these cells are pro-angiogenic. In this study fibroblasts were collected from four PAD patients undergoing carotid endarterectomies. These cells, and neonatal fibroblasts, were transdifferentiated into iECs using modified mRNA. Endothelial phenotype and pro-angiogenic cytokine secretion were investigated. NOD-SCID mice underwent surgery to induce hindlimb ischaemia in a murine model of PAD. Mice received intramuscular injections with either control vehicle, or 1 × 106 neonatal-derived or 1 × 106 patient-derived iECs. Recovery in perfusion to the affected limb was measured using laser Doppler scanning. Perfusion recovery was enhanced in mice treated with neonatal-derived iECs and in two of the three patient-derived iEC lines investigated in vivo. Patient-derived iECs can be successfully generated from PAD patients and for specific patients display comparable pro-angiogenic properties to neonatal-derived iECs.

Antibodies against monocytes and endothelial cells in the sera of patients with atherosclerotic peripheral arterial disease

1988 ◽  
Vol 74 (1-2) ◽  
pp. 99-105 ◽  
Author(s):  
C DANASTASIO ◽  
M IMPALLOMENI ◽  
G MCPHERSON ◽  
W CLEMENTS ◽  
G HOWELLS ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial
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