Identification of geosmin biosynthetic gene in geosmin-producing colonial cyanobacteria Coelosphaerium sp. and isolation of geosmin non-producing Coelosphaerium sp. from brackish Lake Shinji in Japan

Harmful Algae ◽  
2019 ◽  
Vol 84 ◽  
pp. 19-26 ◽  
Author(s):  
Shohei Hayashi ◽  
Shuji Ohtani ◽  
Toshiyuki Godo ◽  
Yukari Nojiri ◽  
Yukiko Saki ◽  
...  
Keyword(s):  
Biosynthetic Gene ◽  
Brackish Lake ◽  
Lake Shinji

scholarly journals Nitrogen mass balance and river concentration dependence in the stock in brackish Lake Shinji

2015 ◽  
Vol 77 (1) ◽  
pp. 47-54
Author(s):  
Hiroshi KAMIYA ◽  
Shogo SUGAHARA ◽  
Yuki SAGA ◽  
Sachiko SATO ◽  
Yukari NOJIRI ◽  
...  
Keyword(s):  
Mass Balance ◽  
Concentration Dependence ◽  
Brackish Lake ◽  
Lake Shinji

scholarly journals Release of nitrogen and phosphorus from sediments observed during a high-frequency field study in brackish Lake Shinji

2015 ◽  
Vol 77 (3) ◽  
pp. 305-313
Author(s):  
Hiroshi KAMIYA ◽  
Shogo SUGAHARA ◽  
Yuki SAGA ◽  
Yukari NOJIRI ◽  
Toshiaki ESUMI ◽  
...  
Keyword(s):  
Field Study ◽  
High Frequency ◽  
High Frequency Field ◽  
Nitrogen And Phosphorus ◽  
Brackish Lake ◽  
Frequency Field ◽  
Lake Shinji

scholarly journals Fluxes of inorganic nutrients from bottom sediments in the brackish Lake Shinji

2012 ◽  
Vol 73 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Ryukichi SUGAI ◽  
Masahiro NISIO ◽  
Isamu MIZOYAMA ◽  
Yasushi SEIKE
Keyword(s):  
Bottom Sediments ◽  
Inorganic Nutrients ◽  
Brackish Lake ◽  
Lake Shinji

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Anacyclamide D8P, a prenylated cyanobactin from a Sphaerospermopsis sp. cyanobacterium

2017 ◽  
Author(s):  
Joana Martins ◽  
Niina Leikoski ◽  
Matti Wahlsten ◽  
Joana Azevedo ◽  
Jorge Antunes ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Cyclic Peptides ◽  
Biosynthetic Gene Cluster ◽  
The Novel ◽  
Tyrosine Residue ◽  
Biosynthetic Gene ◽  
Post Translational Modification ◽  
Biological Assays ◽  
Mass Spectrometric Data ◽  
Spectrometric Data

Cyanobactins are a family of linear and cyclic peptides produced through the post-translational modification of short precursor peptides. Anacyclamides are macrocyclic cyanobactins with a highly diverse sequence that are common in the genus <i>Anabaena</i>. A mass spectrometry-based screening of potential cyanobactin producers led to the discovery of a new prenylated member of this family of compounds, anacyclamide D8P (<b>1</b>), from <i>Sphaerospermopsis</i> sp. LEGE 00249. The anacyclamide biosynthetic gene cluster (<i>acy</i>) encoding the novel macrocyclic prenylated cyanobactin, was sequenced. Heterologous expression of the acy gene cluster in <i>Escherichia</i> <i>coli</i> established the connection between genomic and mass spectrometric data. Unambiguous establishment of the type and site of prenylation required the full structural elucidation of <b>1</b> using Nuclear Magnetic Resonance (NMR), which demonstrated that a forward prenylation occurred on the tyrosine residue. Compound <b>1</b> was tested in pharmacologically or ecologically relevant biological assays and revealed moderate antimicrobial activity towards the fouling bacterium <i>Halomonas aquamarina</i> CECT 5000.<br>

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