scholarly journals Characterization of multiple isolates from an Alexandrium ostenfeldii bloom in The Netherlands

Harmful Algae ◽  
2015 ◽  
Vol 49 ◽  
pp. 94-104 ◽  
Author(s):  
Dedmer B. Van de Waal ◽  
Urban Tillmann ◽  
Helge Martens ◽  
Bernd Krock ◽  
Yvonne van Scheppingen ◽  
...  
Keyword(s):  
The Netherlands ◽  
Alexandrium Ostenfeldii

Prevalence and Determinants of Fecal Colonization with Vancomycin-ResistantEnterococcusin Hospitalized Patients in The Netherlands

2000 ◽  
Vol 21 (8) ◽  
pp. 520-524 ◽  
Author(s):  
Nicole van den Braak ◽  
Alewijn Ott ◽  
Alex van Belkum ◽  
Jan A.J.W. Kluytmans ◽  
Johannes G.M. Koeleman ◽  
...  
Keyword(s):  
Regression Analysis ◽  
The Netherlands ◽  
Hospital Stay ◽  
Analysis Model ◽  
Vancomycin Resistant ◽  
Polymerase Chain ◽  
And Gender ◽  
Prolonged Hospital ◽  
Nosocomial Strain

Objective:To determine the prevalence and determinants of fecal carriage of vancomycin-resistant enterococci (VRE) in intensive care unit (ICU), hematology-oncology, and hemodialysis patients in The Netherlands.Design:Descriptive, multicenter study, with yearly 1-week point-prevalence assessments between 1995 and 1998.Population:All patients hospitalized on the testing days in ICUs and hematology-oncology wards in nine hospitals in The Netherlands were included.Methods:Rectal swabs obtained from 1,112 patients were screened for enterococci in a selective broth and subcultured on selective media with and without 6 mg/L vancomycin. Resistance genotypes were determined by polymerase chain reaction. Further characterization of VRE strains was done by pulsed-field gel electrophoresis (PFGE). We studied possible determinants of VRE colonization with a logistic regression analysis model. Determinants analyzed included gender, age, and log-transformed length of prior hospital stay.Results:The results showed that 614 (55%) of 1,112 patients were colonized with vancomycin-sensitive enterococci, and 15 (1.4%) of 1,112 carried VRE. No increase in VRE colonization was observed from 1995 to 1998. Eleven strains were identified asEnterococcus faeciumand four asEnterococcus faecalis. AllE faeciumand oneE faecaliscarried thevanAgene; the otherE faecalisstrains harbored thevanBgene. PFGE revealed that threevanBVRE isolated from patients hospitalized in one single ICU were related, suggesting nosocomial transmission. Though higher age seemed associated with VRE colonization, exclusion of patients with the nosocomial strain from the regression analysis decreased this relation to nonsignificant. Duration of hospital stay was not associated with VRE colonization.Conclusion:VRE colonization in Dutch hospitals is an infrequent phenomenon. Although nosocomial spread occurs, most observed cases were unrelated, which suggests the possibility of VRE acquisition from outside the hospital. Prolonged hospital stay, age, and gender proved unrelated to VRE colonization.

Chemical Characterization of Airborne Particles in St. Martinus Cathedral in Weert, The Netherlands

2004 ◽  
Vol 145 (1-4) ◽  
pp. 223-227 ◽  
Author(s):  
Anna Worobiec ◽  
Dionne Neilen ◽  
Henk Schellen ◽  
R�ne Van Grieken ◽  
Zoya Spolnik ◽  
...  
Keyword(s):  
The Netherlands ◽  
Chemical Characterization ◽  
Airborne Particles

scholarly journals Mass Spectrometry-Based Characterization of New Spirolides from Alexandrium ostenfeldii (Dinophyceae)

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 505
Author(s):  
Joyce A. Nieva ◽  
Jan Tebben ◽  
Urban Tillmann ◽  
Sylke Wohlrab ◽  
Bernd Krock
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
Molecular Ions ◽  
Ring System ◽  
The Other ◽  
Spectral Techniques ◽  
Mass Spectral ◽  
Alexandrium Ostenfeldii ◽  
Different Strains

Spirolides belong to a group of marine phycotoxins produced by the marine planktonic dinophyte Alexandrium ostenfeldii. Composed of an imine moiety and a spiroketal ring system within a macrocylcle, spirolides are highly diverse with toxin types that vary among different strains. This study aims to characterize the spirolides from clonal A. ostenfeldii strains collected from The Netherlands, Greenland and Norway by mass spectral techniques. The structural characterization of unknown spirolides as inferred from high-resolution mass spectrometry (HR-MS) and collision induced dissociation (CID) spectra revealed the presence of nine novel spirolides that have the pseudo-molecular ions m/z 670 (1), m/z 666 (2), m/z 696 (3), m/z 678 (4), m/z 694 (5), m/z 708 (6), m/z 720 (7), m/z 722 (8) and m/z 738 (9). Of the nine new spirolides proposed in this study, compound 1 was suggested to have a truncated side chain in lieu of the commonly observed butenolide ring in spirolides. Moreover, there is indication that compound 5 might belong to new spirolide subclasses with a trispiroketal ring configuration having a 6:5:6 trispiroketal ring system. On the other hand, the other compounds were proposed as C- and G-type SPX, respectively. Compound 7 is proposed as the first G-type SPX with a 10-hydroxylation as usually observed in C-type SPX. This mass spectrometry-based study thus demonstrates that structural variability of spirolides is larger than previously known and does not only include the presence or absence of certain functional groups but also involves the triketal ring system.

Characterization of the light field in laboratory scale enclosures of eutrophic lake water (Lake Loosdrecht, The Netherlands)

Hydrobiologia ◽  
1992 ◽  
Vol 238 (1) ◽  
pp. 99-109 ◽  
Author(s):  
Herman J. Gons ◽  
Jacco Kromkamp ◽  
Machteld Rijkeboer ◽  
Oscar Schofield
Keyword(s):  
The Netherlands ◽  
Lake Water ◽  
Light Field ◽  
Eutrophic Lake ◽  
Laboratory Scale ◽  
Water Lake ◽  
Eutrophic Lake Water

scholarly journals Comprehensive Characterization of Escherichia coli O104:H4 Isolated from Patients in the Netherlands

2015 ◽  
Vol 6 ◽  
Author(s):  
Mithila Ferdous ◽  
Kai Zhou ◽  
Richard F. de Boer ◽  
Alexander W. Friedrich ◽  
Anna M. D. Kooistra-Smid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
The Netherlands ◽  
Comprehensive Characterization
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