Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays

Harmful Algae ◽  
2014 ◽  
Vol 37 ◽  
pp. 133-141 ◽  
Author(s):  
Bum Soo Park ◽  
Pengbin Wang ◽  
Jin Ho Kim ◽  
Joo-Hwan Kim ◽  
Christopher J. Gobler ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Coastal Waters ◽  
Cochlodinium Polykrikoides ◽  
Pcr Assays

scholarly journals Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 414
Author(s):  
Joseph P. Nowlan ◽  
Scott R. Britney ◽  
John S. Lumsden ◽  
Spencer Russell
Keyword(s):  
Atlantic Salmon ◽  
Quantitative Pcr ◽  
Salmo Salar ◽  
Bacterial Load ◽  
Temporal Distribution ◽  
Quality Parameters ◽  
Antimicrobial Use ◽  
Live Fish ◽  
Dead Fish ◽  
Pcr Assays

Tenacibaculum are frequently detected from fish with tenacibaculosis at aquaculture sites; however, information on the ecology of these bacteria is sparse. Quantitative-PCR assays were used to detect T. maritimum and T. dicentrarchi at commercial Atlantic salmon (Salmo salar) netpen sites throughout several tenacibaculosis outbreaks. T. dicentrarchi and T. maritimum were identified in live fish, dead fish, other organisms associated with netpens, water samples and on inanimate substrates, which indicates a ubiquitous distribution around stocked netpen sites. Before an outbreak, T. dicentrarchi was found throughout the environment and from fish, and T. maritimum was infrequently identified. During an outbreak, increases in the bacterial load in were recorded and no differences were recorded after an outbreak supporting the observed recrudescence of mouthrot. More bacteria were recorded in the summer months, with more mortality events and antibiotic treatments, indicating that seasonality may influence tenacibaculosis; however, outbreaks occurred in both seasons. Relationships were identified between fish mortalities and antimicrobial use to water quality parameters (temperature, salinity, dissolved oxygen) (p < 0.05), but with low R2 values (<0.25), other variables are also involved. Furthermore, Tenacibaculum species appear to have a ubiquitous spatial and temporal distribution around stocked netpen sites, and with the potential to induce disease in Atlantic salmon, continued research is needed.

Abstract 740: Rapid detection of IDH1/2 mutations using RNaseH2 dependent, multiplex quantitative PCR Assays

2017 ◽  
Author(s):  
Yun Bao ◽  
Aymen Baig ◽  
Yu Wang ◽  
A. John Iafrate ◽  
Caifu Chen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Rapid Detection ◽  
Pcr Assays

scholarly journals Establishment and In-House Validation of Simplex and Duplex PCR Methods for Event-Specific Detection of Maize SYN-E3272-5 Using a New Reference Molecule

2010 ◽  
Vol 93 (2) ◽  
pp. 663-675
Author(s):  
Kailin Shen ◽  
Xiang Li ◽  
Shu Wang ◽  
Yingjie Pan ◽  
Zhiyi Shi ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Genetically Modified Organisms ◽  
Insertion Site ◽  
Quantitative Detection ◽  
Duplex System ◽  
Detection Techniques ◽  
Qualitative And Quantitative ◽  
Reference Molecule ◽  
Gm Maize ◽  
Pcr Assays

Abstract Despite rapid developments in the detection techniques for genetically modified organisms (GMOs), the event-specific PCR method with high specificity is still the most used technique. In this study, event-specific simplex and duplex qualitative and quantitative detection systems were developed targeting the 3 insertion site of GM maize SYN-E3272-5 (3272) construct. A reference molecule p3272 was constructed to act as positive control and as calibrator for quantitative analysis. The LOD for simplex and duplex qualitative PCR assays was 10 copies of p3272 control DNA. LOD and the LOQ for simplex and duplex quantitative PCR assays were 10 and 25 copies of p3272 DNA, respectively. Furthermore, four practical GM maize samples were quantified using the established simplex and duplex quantitative PCR systems by in-house validation. Results from five operators showed that the bias ranged from 3.44 to 17.24 in the simplex system and from 0.42 to 16.06 in the duplex system, respectively. These results demonstrated that the established event-specific simplex and duplex qualitative and quantitative PCR systems combined with the reference molecule p3272 are suitable for the detection of GM maize 3272 and its derived products.

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