Variability of paralytic shellfish toxin occurrence and profiles in bivalve molluscs from Great Britain from official control monitoring as determined by pre-column oxidation liquid chromatography and implications for applying immunochemical tests

Harmful Algae ◽  
2014 ◽  
Vol 31 ◽  
pp. 87-99 ◽  
Author(s):  
Andrew D. Turner ◽  
Ben Stubbs ◽  
Lewis Coates ◽  
Monika Dhanji-Rapkova ◽  
Robert G. Hatfield ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Great Britain ◽  
Bivalve Molluscs ◽  
Paralytic Shellfish Toxin ◽  
Official Control ◽  
Paralytic Shellfish ◽  
Shellfish Toxin

Relationship between paralytic shellfish toxin content and sxtA gene copy number in different growth phases of Gymnodinium catenatum (Dinophyceae)

Toxicon ◽  
2021 ◽  
Author(s):  
Armando Mendoza-Flores ◽  
Ignacio Leyva-Valencia ◽  
Francisco E. Hernández-Sandoval ◽  
Clara E. Galindo-Sánchez ◽  
Christine J. Band-Schmidt ◽  
...  
Keyword(s):  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Growth Phases ◽  
Toxin Content ◽  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
Shellfish Toxin ◽  
Gymnodinium Catenatum

Assessment of a Semiquantitative Liquid Chromatography- Fluorescence Detection Method for the Determination of Paralytic Shellfish Poisoning Toxin Levels in Bivalve Molluscs from Great Britain

2014 ◽  
Vol 97 (2) ◽  
pp. 492-497 ◽  
Author(s):  
Andrew D Turner ◽  
Monika Dhanji-Rapkova ◽  
Clothilde Baker ◽  
Myriam Algoet
Keyword(s):  
Great Britain ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Reference Method ◽  
Paralytic Shellfish Poisoning ◽  
Official Method ◽  
Shellfish Poisoning ◽  
Bivalve Molluscs ◽  
Paralytic Shellfish

Abstract AOAC Official Method 2005.06 precolumn oxidation LC-fluorescence detection method has been used for many years for the detection and quantitation of paralytic shellfish poisoning (PSP) toxins in bivalve molluscs. After extensive single- and multiple-laboratory validation, the method has been slowly gaining acceptance worldwide as a useful and practical tool for official control testing. In Great Britain, the method has become routine since 2008, with no requirement since then for reverting back to the bioassay reference method. Although the method has been refined to be semiautomated, faster, and more reproducible, the quantitation step can be complex and time-consuming. An alternative approach was developed to utilize the qualitative screening results for generatinga semiquantitative results assessment. Data obtained over 5 years enabled the comparison of semiquantitative and fully quantitative PSP results in over 15 000 shellfish samples comprising eight different species showed that the semiquantitative approach resulted in over-estimated paralytic shellfish toxin levels by an average factor close to two in comparison with the fully quantified levels. No temporal trends were observed in the data or relating to species type, with the exception of surf clams. The comparison suggested a semiquantitative threshold of 800 μg saxitoxin (STX) eq/kg should provide a safe limitfor the determination of samples to be forwarded to full quantitation. However, the decision was taken to halve this limit to include an additional safety factor of 2, resulting in the use of a semiquantitative threshold of 400 μg STX eq/kg. Implementation of the semiquantitative method into routine testing would result in a significant reduction in the numbers of samples requiring quantitation and have a positive impact on the overall turnaround of reported PSP results. The refined method would be appropriate for any monitoring laboratory faced with high throughput requirements.

scholarly journals The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway

2017 ◽  
Vol 190 ◽  
pp. 133-141 ◽  
Author(s):  
Celina Abi-Khalil ◽  
Darren S. Finkelstein ◽  
Genevieve Conejero ◽  
Justin Du Bois ◽  
Delphine Destoumieux-Garzon ◽  
...  
Keyword(s):  
Immune Cells ◽  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
Shellfish Toxin ◽  
Dependent Pathway

scholarly journals Sources of paralytic shellfish toxin accumulation variability in the Pacific oyster Crassostrea gigas

Toxicon ◽  
2018 ◽  
Vol 144 ◽  
pp. 14-22 ◽  
Author(s):  
Émilien Pousse ◽  
Jonathan Flye-Sainte-Marie ◽  
Marianne Alunno-Bruscia ◽  
Hélène Hégaret ◽  
Fred Jean
Keyword(s):  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
The Pacific ◽  
Shellfish Toxin ◽  
Toxin Accumulation

Identification and characterization of a “biomarker of toxicity” from the proteome of the paralytic shellfish toxin-producing dinoflagellateAlexandrium tamarense (Dinophyceae)

PROTEOMICS ◽  
2006 ◽  
Vol 6 (2) ◽  
pp. 654-666 ◽  
Author(s):  
Leo Lai Chan ◽  
Wai-Hung Sit ◽  
Paul Kwan-Sing Lam ◽  
Dennis Paul Hsientang Hsieh ◽  
Ivor John Hodgkiss ◽  
...  
Keyword(s):  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
Shellfish Toxin ◽  
Identification And Characterization

Paralytic shellfish toxin uptake, tissue distribution, and depuration in the Southern Rock Lobster Jasus edwardsii Hutton

Harmful Algae ◽  
2020 ◽  
Vol 95 ◽  
pp. 101818 ◽  
Author(s):  
Alison Turnbull ◽  
Navreet Malhi ◽  
Andreas Seger ◽  
Tim Harwood ◽  
Jessica Jolley ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Rock Lobster ◽  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
Jasus Edwardsii ◽  
Shellfish Toxin ◽  
Toxin Uptake

Connections and clustering of Paralytic Shellfish Toxin events among coastal embayments in an archipelago partly mediated by advection

Harmful Algae ◽  
2022 ◽  
Vol 111 ◽  
pp. 102147
Author(s):  
Andalus T. Punongbayan ◽  
Ysabel D. Wang ◽  
Cesar L. Villanoy ◽  
Aletta T. Yñiguez
Keyword(s):  
Paralytic Shellfish Toxin ◽  
Paralytic Shellfish ◽  
Coastal Embayments ◽  
Shellfish Toxin
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