In situ life-cycle stages of Alexandrium fundyense during bloom development in Northport Harbor (New York, USA)

Harmful Algae ◽  
2012 ◽  
Vol 16 ◽  
pp. 20-26 ◽  
Author(s):  
Sílvia Anglès ◽  
Esther Garcés ◽  
Theresa K. Hattenrath-Lehmann ◽  
Christopher J. Gobler
Keyword(s):  
New York ◽  
Life Cycle ◽  
Alexandrium Fundyense ◽  
Life Cycle Stages

In situ observations of Akashiwo sanguinea (Dinophyceae) displaying life cycle stages during blooms in a subtropical estuary

2017 ◽  
Vol 60 (6) ◽  
Author(s):  
Susan Badylak ◽  
Edward J. Phlips ◽  
Ashley Loren Mathews ◽  
Karen Kelley
Keyword(s):  
Life Cycle ◽  
Algal Bloom ◽  
Harmful Algal Bloom ◽  
Life Cycle Stages ◽  
Subtropical Estuary ◽  
In Situ Observations ◽  
Akashiwo Sanguinea

AbstractThis study reports on the harmful algal bloom (HAB) dinoflagellate

scholarly journals Microtubule polyglutamylation is important for regulating cytoskeletal architecture and motility in Trypanosoma brucei

2020 ◽  
Vol 133 (18) ◽  
pp. jcs248047 ◽  
Author(s):  
Jana Jentzsch ◽  
Adal Sabri ◽  
Konstantin Speckner ◽  
Gertrud Lallinger-Kube ◽  
Matthias Weiss ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Life Cycle ◽  
Trypanosoma Brucei ◽  
Dynamic Behaviour ◽  
Cell Movement ◽  
Post Translational Modifications ◽  
Life Cycle Stages ◽  
Associated Proteins ◽  
Flagellar Axoneme

ABSTRACTThe shape of kinetoplastids, such as Trypanosoma brucei, is precisely defined during the stages of the life cycle and governed by a stable subpellicular microtubule cytoskeleton. During the cell cycle and transitions between life cycle stages, this stability has to transiently give way to a dynamic behaviour to enable cell division and morphological rearrangements. How these opposing requirements of the cytoskeleton are regulated is poorly understood. Two possible levels of regulation are activities of cytoskeleton-associated proteins and microtubule post-translational modifications (PTMs). Here, we investigate the functions of two putative tubulin polyglutamylases in T. brucei, TTLL6A and TTLL12B. Depletion of both proteins leads to a reduction in tubulin polyglutamylation in situ and is associated with disintegration of the posterior cell pole, loss of the microtubule plus-end-binding protein EB1 and alterations of microtubule dynamics. We also observe a reduced polyglutamylation of the flagellar axoneme. Quantitative motility analysis reveals that the PTM imbalance correlates with a transition from directional to diffusive cell movement. These data show that microtubule polyglutamylation has an important role in regulating cytoskeletal architecture and motility in the parasite T. brucei.This article has an associated First Person interview with the first author of the paper.

Intron-containing β-tubulin transcripts in Cryptosporidium parvum cultured in vitro

Microbiology ◽  
2004 ◽  
Vol 150 (5) ◽  
pp. 1191-1195 ◽  
Author(s):  
Xiaomin Cai ◽  
Cheryl A. Lancto ◽  
Mitchell S. Abrahamsen ◽  
Guan Zhu
Keyword(s):  
Life Cycle ◽  
Cryptosporidium Parvum ◽  
Late Life ◽  
Host Cells ◽  
Intron Splicing ◽  
Transcript Processing ◽  
Life Cycle Stages ◽  
Tubulin Mrna

The genome of Cryptosporidium parvum contains a relatively small number of introns, which includes the β-tubulin gene with only a single intron. Recently, it was observed that the intron was not removed from some of the β-tubulin transcripts in the late life cycle stages cultured in vitro. Although normally spliced β-tubulin mRNA was detected in all parasite intracellular stages by RT-PCR (e.g. HCT-8 or Caco-2 cells infected with C. parvum for 12–72 h), at 48–72 h post-infection unprocessed β-tubulin transcripts containing intact introns started to appear in parasite mRNA within infected host cells. The intron-containing transcripts could be detected by fluorescence in situ hybridization (FISH) using an intron-specific probe. The intron-containing β-tubulin transcripts appeared unique to the in vitro-cultured C. parvum, since they were not detected in parasite-infected calves at 72 h. As yet, it is unclear whether the late life cycle stages of C. parvum are partially deficient in intron-splicing or the intron-splicing processes have merely slowed, both of which would allow the detection of intron-containing transcripts. Another possible explanation is that the decay in transcript processing might simply be due to the onset of parasite death. Nonetheless, the appearance of intron-containing transcripts coincides with the arrest of C. parvum development in vitro. This unusual observation prompts speculation that the abnormal intron-splicing of β-tubulin transcripts may be one of the factors preventing complete development of this parasite in vitro. Furthermore, the presence of both processed and unprocessed introns in β-tubulin transcripts in vitro may provide a venue for studying overall mechanisms for intron-splicing in this parasite.

Differences in swimming pattern between life cycle stages of the toxic dinoflagellate Alexandrium fundyense

Harmful Algae ◽  
2013 ◽  
Vol 21-22 ◽  
pp. 36-43 ◽  
Author(s):  
Agneta Persson ◽  
Barry C. Smith ◽  
Gary H. Wikfors ◽  
Jennifer H. Alix
Keyword(s):  
Life Cycle ◽  
Toxic Dinoflagellate ◽  
Alexandrium Fundyense ◽  
Life Cycle Stages ◽  
Swimming Pattern

scholarly journals Light-Dependent Transcriptional Regulation of Genes of Biogeochemical Interest in the Diploid and Haploid Life Cycle Stages of Emiliania huxleyi

2009 ◽  
Vol 75 (10) ◽  
pp. 3366-3369 ◽  
Author(s):  
Sophie Richier ◽  
Marie-Emmanuelle Kerros ◽  
Colomban de Vargas ◽  
Liti Haramaty ◽  
Paul G. Falkowski ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Life Cycle ◽  
Emiliania Huxleyi ◽  
Life Stages ◽  
Dark Cycle ◽  
Expression Of Genes ◽  
Life Cycle Stages

ABSTRACT The expression of genes of biogeochemical interest in calcifying and noncalcifying life stages of the coccolithophore Emiliania huxleyi was investigated. Transcripts potentially involved in calcification were tested through a light-dark cycle. These transcripts were more abundant in calcifying cells and were upregulated in the light. Their application as potential candidates for in situ biogeochemical proxies is also suggested.

FINANCING THE DEVELOPMENT OF MUNICIPAL ENTITIES AT DIFFERENT LIFE CYCLE STAGES: PUBLIC AND PRIVATE RESOURCES

2019 ◽  
Vol 0 (3) ◽  
pp. 53-60 ◽  
Author(s):  
T.Yu. Altufyeva ◽  
◽  
P.A. Ivanov ◽  
G.R. Sakhapova ◽  
◽  
...  
Keyword(s):  
Life Cycle ◽  
Public And Private ◽  
Life Cycle Stages

Mathematical Model Investigation of Far-Field Transport of Ocean-Dumped Sewage Sludge Related to Remote Sensing

1982 ◽  
Vol 14 (3) ◽  
pp. 33-39
Author(s):  
C Y Kuo
Keyword(s):  
New York ◽  
Sewage Sludge ◽  
Laboratory Data ◽  
Three Dimensional ◽  
Approximate Model ◽  
Transport Processes ◽  
Far Field ◽  
New York Bight ◽  
Mean Current

An existing, three-dimensional, Eulerian-Lagrangian finite-difference model was modified and used to examine the far-field transport processes of dumped sewage sludge in the New York Bight. Both in situ and laboratory data were utilized in an attempt to approximate model inputs such as mean current speed, vertical and horizontal diffusion coefficients, particle size distributions, and specific gravities. Concentrations of the sludge near the sea surface predicted from the computer model were compared qualitatively with those remotely sensed.

scholarly journals COMPETITIVE EFFECTS OF THE ALIEN INVASIVE CENTAUREA SOLSTITIALISL. ON TWO CHILEAN BACCHARIS SPECIES AT DIFFERENT LIFE-CYCLE STAGES

2009 ◽  
Vol 66 (1) ◽  
Author(s):  
Susana Gómez-González ◽  
Lohengrin A Cavieres ◽  
Patricio Torres ◽  
Cristian Torres-Díaz
Keyword(s):  
Life Cycle ◽  
Competitive Effects ◽  
Life Cycle Stages
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