Detection of the toxic marine diatom Pseudo-nitzschia multiseries using the RuBisCO small subunit (rbcS) gene in two real-time RNA amplification formats

Harmful Algae ◽  
2011 ◽  
Vol 11 ◽  
pp. 54-64 ◽  
Author(s):  
Jennifer A. Delaney ◽  
Robert M. Ulrich ◽  
John H. Paul
Keyword(s):  
Real Time ◽  
Small Subunit ◽  
Marine Diatom ◽  
Rna Amplification ◽  
Rubisco Small Subunit ◽  
Rbcs Gene

Cell-Specific Expression of Rubisco Small Subunit and Rubisco Activase Genes in C3 and C4 Species of Atriplex

1992 ◽  
Vol 19 (1) ◽  
pp. 89 ◽  
Author(s):  
GS Hudson ◽  
RE Dengler ◽  
PW Hattersley ◽  
G Dengler
Keyword(s):  
In Situ Hybridisation ◽  
Rubisco Activase ◽  
Small Subunit ◽  
Bundle Sheath ◽  
Specific Expression ◽  
Bisphosphate Carboxylase ◽  
C4 Species ◽  
Rubisco Small Subunit ◽  
Rbcs Gene

In situ hybridisation techniques have been used to determine the distribution of mRNAs for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) and Rubisco activase in leaves of Atriplex patula L. (C3) and A. rosea L. (C4). In A. patula, mRNA for Rubisco small subunit (encoded by the rbcS gene family) was found to accumulate in the mesophyll and bundle sheath, while in A. rosea it accumulated in the bundle sheath only, as shown previously for the C4 monocot Zea mays L. The spatial distribution of rca transcripts for Rubisco activase paralleled that for the rbcS transcripts in both C3 and C4 Atriplex species, providing evidence that Rubisco activase is required in cells only where Rubisco is present.

A NASBA on microgel-tethered molecular-beacon microarray for real-time microbial molecular diagnostics

The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Y. Ma ◽  
X. Dai ◽  
T. Hong ◽  
G. B. Munk ◽  
M. Libera
Keyword(s):  
Real Time ◽  
Bloodstream Infection ◽  
Molecular Diagnostics ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Infection Model ◽  
Rna Amplification ◽  
Microbial Detection

Gel-tethered molecular beacons coupled with NASBA RNA amplification enable real-time microbial detection and differentiation in a bloodstream infection model.

Structure and Expression of the Euglena rbcS Gene Coding for the Small Subunit of Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase

1992 ◽  
pp. 641-644
Author(s):  
Weil Jacques-Henry ◽  
Chan Raquel Lia ◽  
Keller Mario ◽  
Tessier Luc-Henri ◽  
Imbault Patrice
Keyword(s):  
Small Subunit ◽  
Bisphosphate Carboxylase ◽  
Gene Coding ◽  
Rbcs Gene

scholarly journals A Real-Time PCR Assay for Simultaneous Detection and Differentiation of Four Common Entamoeba Species That Infect Humans

2020 ◽  
Vol 59 (1) ◽  
pp. e01986-20
Author(s):  
Ibne Karim M. Ali ◽  
Shantanu Roy
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Small Subunit ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Content Type ◽  
Rdna Sequences ◽  
Appropriate Treatment

ABSTRACTThere are over 40 species within the genus Entamoeba, eight of which infect humans. Of these, four species (Entamoeba histolytica, E. dispar, E. moshkovskii, and E. bangladeshi) are morphologically indistinguishable from each other, and yet differentiation is important for appropriate treatment decisions. Here, we developed a hydrolysis probe-based tetraplex real-time PCR assay that can simultaneously detect and differentiate these four species in clinical samples. In this assay, multicopy small-subunit (SSU) ribosomal DNA (rDNA) sequences were used as targets. We determined that the tetraplex real-time PCR can detect amebic DNA corresponding to as little as a 0.1 trophozoite equivalent of any of these species. We also determined that this assay can detect E. histolytica DNA in the presence of 10-fold more DNA from another Entamoeba species in mixed-infection scenarios. With a panel of more than 100 well-characterized clinical samples diagnosed and confirmed using a previously published duplex real-time PCR (capable of detecting E. histolytica and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity and specificity comparable with those demonstrated by the duplex real-time PCR assay. The advantage of our assay over the duplex assay is that it can specifically detect two additional Entamoeba species and can be used in conventional PCR format. This newly developed assay will allow further characterization of the epidemiology and pathogenicity of the four morphologically identical Entamoeba species, especially in low-resource settings.

scholarly journals A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae

2020 ◽  
Vol 119 (11) ◽  
pp. 3909-3913
Author(s):  
Zaida Rentería-Solís ◽  
Tran Nguyen-Ho-Bao ◽  
Shahinaz Taha ◽  
Arwid Daugschies
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Small Subunit ◽  
Sybr Green ◽  
Pcr Assay ◽  
Standard Curve ◽  
Trichomonas Gallinae ◽  
Host Interaction

Abstract Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies.

scholarly journals Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivumL.)

2015 ◽  
Vol 10 (2) ◽  
pp. e989033 ◽  
Author(s):  
Shalini Mukherjee ◽  
Claudio Stasolla ◽  
Anita Brûlé-Babel ◽  
Belay T Ayele
Keyword(s):  
Common Wheat ◽  
Gene Promoter ◽  
Small Subunit ◽  
Subunit Gene ◽  
Isolation And Characterization ◽  
Rubisco Small Subunit

scholarly journals The Rubisco Small Subunit Is Involved in Tobamovirus Movement and Tm-22-Mediated Extreme Resistance

2012 ◽  
Vol 161 (1) ◽  
pp. 374-383 ◽  
Author(s):  
Jinping Zhao ◽  
Qi Liu ◽  
Haili Zhang ◽  
Qi Jia ◽  
Yiguo Hong ◽  
...  
Keyword(s):  
Small Subunit ◽  
Extreme Resistance ◽  
Rubisco Small Subunit
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