scholarly journals Flow cytometric measurements of cellular responses in a toxic dinoflagellate, Alexandrium minutum, upon exposure to thermal, chemical and mechanical stresses

Harmful Algae ◽  
2011 ◽  
Vol 10 (5) ◽  
pp. 463-471 ◽  
Author(s):  
Hansy Haberkorn ◽  
Hélène Hégaret ◽  
Dominique Marie ◽  
Christophe Lambert ◽  
Philippe Soudant
Keyword(s):  
Cellular Responses ◽  
Mechanical Stresses ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum ◽  
Flow Cytometric

scholarly journals Flow cytometric assessment of morphology, viability, and production of reactive oxygen species ofCrassostrea gigasoocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure

2014 ◽  
Vol 85 (12) ◽  
pp. 1049-1056 ◽  
Author(s):  
Nelly Le Goïc ◽  
Hélène Hégaret ◽  
Myrina Boulais ◽  
Jean-Philippe Béguel ◽  
Christophe Lambert ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum ◽  
Flow Cytometric

scholarly journals Relative grazing impact of microzooplankton and mesozooplankton on a bloom of the toxic dinoflagellate Alexandrium minutum

2003 ◽  
Vol 259 ◽  
pp. 303-309 ◽  
Author(s):  
A Calbet ◽  
D Vaqué ◽  
J Felipe ◽  
M Vila ◽  
M Montserrat Sala ◽  
...  
Keyword(s):  
Grazing Impact ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum

scholarly journals The toxic dinoflagellate Alexandrium minutum impairs the performance of oyster embryos and larvae

Harmful Algae ◽  
2020 ◽  
Vol 92 ◽  
pp. 101744 ◽  
Author(s):  
Justine Castrec ◽  
Hélène Hégaret ◽  
Matthias Huber ◽  
Jacqueline Le Grand ◽  
Arnaud Huvet ◽  
...  
Keyword(s):  
Toxic Dinoflagellate ◽  
Alexandrium Minutum

scholarly journals Nuclear and Cell Morphological Changes during the Cell Cycle and Growth of the Toxic Dinoflagellate Alexandrium minutum

Protist ◽  
2015 ◽  
Vol 166 (1) ◽  
pp. 146-160 ◽  
Author(s):  
Carlos Dapena ◽  
Isabel Bravo ◽  
Angeles Cuadrado ◽  
Rosa Isabel Figueroa
Keyword(s):  
Cell Cycle ◽  
Morphological Changes ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum

scholarly journals Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1V

2006 ◽  
Vol 56 (8) ◽  
pp. 1991-1995 ◽  
Author(s):  
Lucía Palacios ◽  
David R. Arahal ◽  
Beatriz Reguera ◽  
Irma Marín
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum

A Gram-negative, aerobic, non-symbiotic bacterium (AM1V30T) was isolated from the toxic dinoflagellate Alexandrium minutum AL1V. On the basis of 16S rRNA gene sequence similarity, strain AM1V30T was most closely related (97.4 % similarity) to the type strain of Hoeflea marina, which belongs to the family Phyllobacteriaceae within the order Rhizobiales of the class Alphaproteobacteria. A polyphasic approach was used to clarify the taxonomic position of strain AM1V30T. During the course of this study, a second species was described by others as belonging to the genus Hoeflea, namely Hoeflea phototrophica; it showed a somewhat higher level of 16S rRNA gene sequence similarity with respect to strain AM1V30T (98.2 %) and was also taken into account. The fatty acid profiles, physiological and biochemical data and DNA G+C content (59.7 mol%) support the classification of strain AM1V30T as a member of the genus Hoeflea. The characteristics of the novel strain were sufficiently distinct to indicate that it represents a separate species. To confirm this conclusion, DNA–DNA hybridizations were performed: low values (between 15.8 and 29.8 %) were obtained in all cases. Thus, AM1V30T represents a novel species within the genus Hoeflea, for which the name Hoeflea alexandrii sp. nov. is proposed. Strain AM1V30T (=CECT 5682T=DSM 16655T) is the type strain.

First Report of Parasitism on the Toxic Dinoflagellate Alexandrium minutum Halim

2000 ◽  
Vol 50 (1) ◽  
pp. 109-113 ◽  
Author(s):  
E. Erard-Le Denn ◽  
M.-J. Chrétiennot-Dinet ◽  
I. Probert
Keyword(s):  
Toxic Dinoflagellate ◽  
Alexandrium Minutum ◽  
First Report

A comparison of potency of and intracellular signaling events provoked by, small molecule and protein-based mpl agonists

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e14624-e14624
Author(s):  
C. Hartley ◽  
G. Dyas ◽  
I. Archibeque ◽  
A. Al-Assaad ◽  
B. Sun ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Small Molecule ◽  
Intracellular Signaling ◽  
Proliferative Response ◽  
Cellular Responses ◽  
Human Cd34 ◽  
Flow Cytometric ◽  
Similar Dose ◽  
Cd34 Cells ◽  
Atp Bioluminescence

e14624 Background: Thrombopoietin (TPO) interacts with its receptor (mpl) to initiate signal transduction and increase platelet production. Other mpl agonists have been developed for clinical use: MDGF (a recombinant protein) and romiplostim (a peptibody) are protein-based mpl agonists; eltrombopag (a small molecule) is a nonpeptide mpl agonist. Methods: Mpl agonists were compared for their ability to induce cellular responses and activate relevant signaling pathways in mpl-expressing cells. Proliferation was assessed by ATP bioluminescence in Baf3/mpl cells treated with mpl agonists for 2 days. Differentiation was assessed by flow cytometric-detection of CD41 and CD61 expression on human CD34+ cells cultured with mpl agonists for 7 days. Activation of signaling pathways was determined by Western blot analysis of proteins from serum-starved Baf3/mpl cells treated with mpl agonists for 20 minutes. Experiments were repeated 3 or more times. Results: The TPO concentration that produced a half-maximal (EC50) proliferative response of Baf3/mpl cells was comparable with that of romiplostim (Table). In contrast, a 30-fold greater concentration of MGDF and a 2,000-fold greater concentration of small molecule were required to produce the same degree of proliferation. In addition, a 1,000,000-fold greater concentration of small molecule compared with romiplostim was required to produce an EC50 for differentiation of CD34+ cells. Mpl agonists activated identical signaling pathways in Baf3/mpl cells: a similar dose-response for phosphorylation of JAK2, Stat3, Stat5, Erk1/2, and AKT was observed at their respective EC10, EC50, and EC100 concentrations. Conclusions: In Baf3/mpl cells, the small molecule was between 1,000- and 1,000,000-fold less potent than protein-based mpl agonists when corrected for molecular mass differences. Small molecule and protein-based mpl agonists were indistinguishable when mpl intracellular signaling was compared at similarly effective concentrations. [Table: see text] [Table: see text]

scholarly journals Development of microsatellite markers in the toxic dinoflagellate Alexandrium minutum (Dinophyceae)

2006 ◽  
Vol 6 (3) ◽  
pp. 756-758 ◽  
Author(s):  
S. NAGAI ◽  
L. MCCAULEY ◽  
N. YASUDA ◽  
D. L. ERDNER ◽  
D. M. KULIS ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Toxic Dinoflagellate ◽  
Alexandrium Minutum
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