Nutrition and growth kinetics in nitrogen- and phosphorus-limited cultures of the novel red tide flagellate Chattonella ovata (Raphidophyceae)

Harmful Algae ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Haruo Yamaguchi ◽  
Setsuko Sakamoto ◽  
Mineo Yamaguchi
Keyword(s):  
Growth Kinetics ◽  
Red Tide ◽  
The Novel ◽  
Nitrogen And Phosphorus

scholarly journals Active reconfiguration of cytoplasmic lipid droplets governs migration of nutrient-limited phytoplankton

2021 ◽  
Author(s):  
Anupam Sengupta ◽  
Jayabrata Dhar ◽  
Francesco Danza ◽  
Arkajyoti Ghoshal ◽  
Sarah Elisabeth Mueller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Red Tide ◽  
Mechanical Energy ◽  
Optimal Growth ◽  
Active Role ◽  
Phosphorus Limitation ◽  
Vertical Movement ◽  
Nitrogen And Phosphorus ◽  
And Migration ◽  
Motile Species

As open oceans continue to warm, modified currents and enhanced stratification exacerbate nitrogen and phosphorus limitation, constraining primary production. The ability to migrate vertically bestows motile phytoplankton a crucial – albeit energetically expensive – advantage toward vertically redistributing for optimal growth, uptake and resource storage in nutrient-limited water columns. However, this traditional view discounts the possibility that phytoplankton migration may be actively selected by the storage dynamics when nutrients turn limiting. Here we report that storage and migration in phytoplankton are coupled traits, whereby motile species harness energy storing lipid droplets (LDs) to biomechanically regulate migration in nutrient limited settings. LDs grow and translocate directionally within the cytoplasm to accumulate below the cell nucleus, tuning the speed, trajectory and stability of swimming cells. Nutrient reincorporation reverses the LD translocation, restoring the homeostatic migratory traits measured in population-scale millifluidic experiments. Combining intracellular LD tracking and quantitative morphological analysis of red-tide forming alga, Heterosigma akashiwo , along with a model of cell mechanics, we discover that the size and spatial localization of growing LDs govern the ballisticity and orientational stability of migration. The strain-specific shifts in migration which we identify here are amenable to a selective emergence of mixotrophy in nutrient-limited phytoplankton. We rationalize these distinct behavioral acclimatization in an ecological context, relying on concomitant tracking of the photophysiology and reactive oxygen species (ROS) levels, and propose a dissipative mechanical energy budget for motile phytoplankton for alleviating nutrient limitation. The emergent resource acquisition strategies, enabled by distinct strain-specific migratory acclimatizing mechanisms, highlight the active role of the reconfigurable cytoplasmic LDs in vertical movement. By uncovering a mechanistic coupling between dynamics of intracellular changes to physiologically governed migration strategies, this work offers a tractable framework to delineate diverse strategies which phytoplankton may harness to maximize fitness and resource pool in nutrient-limited open oceans of the future.

Effects of temperature, salinity and irradiance on growth of the novel red tide flagellate Chattonella ovata (Raphidophyceae)

Harmful Algae ◽  
2010 ◽  
Vol 9 (4) ◽  
pp. 398-401 ◽  
Author(s):  
Haruo Yamaguchi ◽  
Koichiro Mizushima ◽  
Setsuko Sakamoto ◽  
Mineo Yamaguchi
Keyword(s):  
Red Tide ◽  
The Novel ◽  
Effects Of Temperature

Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

2019 ◽  
Vol 55 (5) ◽  
pp. 1154-1165
Author(s):  
Yevhen Maltsev ◽  
Irina Maltseva ◽  
Svetlana Maltseva ◽  
John Patrick Kociolek ◽  
Maxim Kulikovskiy
Keyword(s):  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
The Novel ◽  
Nitrogen And Phosphorus ◽  
Reduced Nitrogen

scholarly journals Lipid accumulation by Coelastrella multistriata (Scenedesmaceae, Sphaeropleales) during nitrogen and phosphorus starvation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yevhen Maltsev ◽  
Zinaida Krivova ◽  
Svetlana Maltseva ◽  
Kateryna Maltseva ◽  
Elena Gorshkova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Phylogenetic Analysis ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Basal Medium ◽  
The Novel ◽  
Fatty Acid Profiles ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Starvation ◽  
Long Chain

AbstractA novel freshwater strain of Coelastrella multistriata MZ–Ch23 was discovered in Tula region, Russia. The identification is based on morphological features, phylogenetic analysis of SSU rDNA gene and ITS1–5.8S rDNA–ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis places the novel strain in the “core” Coelastrella clade within the Chlorophyceae. This is the first record of Coelastrella multistriata in the algal flora of Russia. Cultivation experiments were carried out to evaluate growth dynamics of the newly identified strain and the impact of nitrogen and/or phosphorus depletion on the fatty acid profiles and lipid productivity. On the fully supplemented Bold’s basal medium and under phosphorus-depleted conditions as well, the fatty acid profiles were dominated by α-linolenic acid (29.4–38.1% of total fatty acids). Depletion of either nitrogen or both nitrogen and phosphorus was associated with increased content of oleic acid (32.9–33.7%) and linoleic acid (11.9%). Prolongation of the growth to two months (instead of 25 days) resulted in increased content and diversity of very long-chain fatty acids including saturated species. The total very long-chain fatty acid content of 9.99% achieved in these experiments was 1.9–12.3-fold higher than in stress experiments. The highest variation was observed for oleic acid (3.4–33.7%). The novel strain showed the ability to accumulate lipids in amounts up to 639.8 mg L−1 under nitrogen and phosphorus starvation, which exceeds the previously obtained values for most Coelastrella strains. Thus, the newly identified MZ–Ch23 strain can be considered as a potential producer of omega-3 fatty acids on fully supplemented Bold’s basal medium or as a source of biomass with high content of saturated and monounsaturated fatty acids after nitrogen and phosphorus starvation.

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