Genetic variability among Alexandrium tamarense and Alexandrium minutum strains studied by RAPD banding pattern analysis

Harmful Algae ◽  
2006 ◽  
Vol 5 (5) ◽  
pp. 599-607 ◽  
Author(s):  
Rosa Martínez ◽  
Carolina Añíbarro ◽  
Sonsoles Fernández
Author(s):  
Muthusamy Govarthanan ◽  
Arunapriya S ◽  
Guruchandar A ◽  
Selvankumar T ◽  
Gnanasekaran N ◽  
...  

1974 ◽  
Vol 13 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
C.-C. Lin

1991 ◽  
Vol 11 (9) ◽  
pp. 4572-4580
Author(s):  
C W Greider

Telomerase synthesizes tandem repeats of the sequence d(TTGGGG) onto input d(TTGGGG)n primer oligonucleotides (C. W. Greider and E. H. Blackburn, Cell 43:405-413). An intrinsic RNA component of the enzyme provides the template for d(TTGGGG)n repeat synthesis [C. W. Greider and E. H. Blackburn, Nature (London) 337:331-337, 1989; G.-L. Lu, J. D. Bradley, L. D. Attardi, and E. H. Blackburn, Nature (London) 344:126-132, 1990]. In a typical reaction, products greater than 2,000 nucleotides were synthesized in 60 min. Dilution and primer challenge experiments showed that these long products were synthesized processively. The apparent processivity was not due to a higher affinity of the enzyme for long d(TTGGGG) products over the shorter competitors. The degree of processivity was quantitated; telomerase synthesized approximately 520 nucleotides before half of the enzyme had dissociated. After dissociating, telomerase reinitiated d(TTGGGG)n synthesis on new primer oligonucleotides. The products from a telomerase reaction have a characteristic 6-nucleotide banding pattern (C. W. Greider and E. H. Blackburn, Cell 51:887-898, 1987). A strong pause in the reaction occurs after the addition of the first G in the sequence d(TTGGGG). Both the processivity and the banding pattern analysis imply that in the elongation mechanism there must be a translocation step after the 9 nucleotides of internal template RNA have been copied to the extreme 5' end.


1982 ◽  
Vol 33 (2) ◽  
pp. 243 ◽  
Author(s):  
PJ Goyne ◽  
GL Hammer ◽  
DR Woodruff

The phenology of commercial sunflower cultivars available to Queensland growers in 1979 was studied in monthly plantings over a 12-month period at Toowoomba in southern Queensland. By using pattern analysis procedures, the cultivars were classified into three maturity groups, viz. 'Very Quick', 'Quick' and 'Medium', based on the number of days from emergence to the head-visible stage of growth. Most of the cultivars belonged to the Quick and Medium maturity groups. Cultivar differences were most obvious for plantings in the cooler months (March to October). To classify new releases, one or two plantings during this period as well as one planting in summer is recommended. Sunfola 68-2 and Hysun 30 should be included in these plantings as cultivars representative of the two major maturity groups. The study showed that there was very little genetic variability in phenology in the present commercial cultivars and there was little difference in phenology among cultivars in the main summer planting period.


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