Diarrhetic shellfish poisoning (DSP) toxins in South Australian shellfish

Harmful Algae ◽  
2006 ◽  
Vol 5 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Thomas L. Madigan ◽  
Ken G. Lee ◽  
David J. Padula ◽  
Paul McNabb ◽  
Andrew M. Pointon
Keyword(s):  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Dsp Toxins

Occurrence of diarrhetic shellfish poisoning (DSP) toxins in clams (Ruditapes decussatus) from Tunis north lagoon

2011 ◽  
Vol 184 (8) ◽  
pp. 5085-5095 ◽  
Author(s):  
Zina Armi ◽  
Souad Turki ◽  
Elbahri Trabelsi ◽  
Alfiero Ceredi ◽  
Elena Riccardi ◽  
...  
Keyword(s):  
Ruditapes Decussatus ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Dsp Toxins

Analysis of Diarrhetic Shellfish Poisoning Toxins in Shellfish Tissue by Liquid Chromatography with Fluorometric and Mass Spectrometric Detection

1995 ◽  
Vol 78 (2) ◽  
pp. 555-569 ◽  
Author(s):  
Michael A Quilliam
Keyword(s):  
Liquid Chromatography ◽  
Complex Mixture ◽  
Internal Standard ◽  
Fluorometric Detection ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Mussel Tissue ◽  
Accuracy And Precision ◽  
Volumetric Errors ◽  
Dsp Toxins

Abstract Diarrhetic shellfish poisoning (DSP) is a severe gastrointestinal illness caused by consumption of shellfish contaminated with toxigenic dinoflagellates. The main toxins responsible for DSP are okadaic acid (OA), DTX-1, DTX-2, and DTX-3, the latter being a complex mixture of 7-O-acyl derivatives of the first 3. In this study, existing methods based on liquid chromatography (LC) combined with mass spectrometry (LC–MS) and LC with fluorometric detection (LC–FLD) of anthryldiazomethane (ADAM) derivatives were improved upon to achieve a high degree of accuracy and precision for the determination of DSP toxins in a new mussel tissue reference material (MUS-2). All experimental parameters were examined comprehensively, and a new internal standard and a new solid-phase extraction cleanup method were introduced. Quantitative extraction of DSP toxins from shellfish tissue was achieved by exhaustive extraction with aqueous 80% methanol. Cleanup was accomplished by partitioning the crude aqueous methanol extract with hexane to remove lipids and then with chloroform to isolate the toxins. A further cleanup based on an aminopropylsilica column was useful for LC-MS and looks promising for the ADAM/LC-FLD method. The internal standard, 7-O-acetylokadaic acid, synthesized by partial acetylation of OA, improved accuracy and precision by correcting for incomplete recoveries in extraction, cleanup, and derivatization steps and for volumetric errors and instrumental drift. An improved silica cleanup after ADAM derivatization also was developed by controlling the activities of both sorbent and solvents. The methods were tested with various mussel tissue samples. The resulting improved methods will be useful to analysts involved in routine monitoring of DSP tox ins.

Effect of the industrial canning on the toxicity of mussels contaminated with diarrhetic shellfish poisoning (DSP) toxins

Toxicon ◽  
2016 ◽  
Vol 112 ◽  
pp. 1-7 ◽  
Author(s):  
Juan Blanco ◽  
Fabiola Arévalo ◽  
Jorge Correa ◽  
M. Corina Porro ◽  
Ana G. Cabado ◽  
...  
Keyword(s):  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Dsp Toxins

scholarly journals Integrated Enzyme-Linked Immunosorbent Assay Screening System for Amnesic, Neurotoxic, Diarrhetic, and Paralytic Shellfish Poisoning Toxins Found in New Zealand

2001 ◽  
Vol 84 (5) ◽  
pp. 1643-1648 ◽  
Author(s):  
Ian Garthwaite ◽  
Kathryn M Ross ◽  
Christopher O Miles ◽  
Lyn R Briggs ◽  
Neale R Towers ◽  
...  
Keyword(s):  
New Zealand ◽  
Immunosorbent Assay ◽  
Paralytic Shellfish Poisoning ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening System ◽  
Good Recovery ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Paralytic Shellfish ◽  
Dsp Toxins

Abstract Enzyme-linked immunosorbent assays (ELISAs) were developed for amnesic, neurotoxic, and diarrhetic shellfish poisoning (ASP, NSP, and DSP) toxins and for yessotoxin. These assays, along with a commercially available paralytic shellfish poisoning (PSP) ELISA, were used to test the feasibility of an ELISA-based screening system. It was concluded that such a system to identify suspect shellfish samples, for subsequent analysis by methods approved by international regulatory authorities, is feasible. The assays had sufficient sensitivity and can be used on simple shellfish extracts. Alcohol extraction gave good recovery of all toxin groups. The ease of ELISAs permits the ready expansion of the system to screen for other toxins, as new ELISAs become available.

Sulfated diesters of okadaic acid and DTX-1: Self-protective precursors of diarrhetic shellfish poisoning (DSP) toxins

Harmful Algae ◽  
2017 ◽  
Vol 63 ◽  
pp. 85-93 ◽  
Author(s):  
Tingmo Hu ◽  
Patricia LeBlanc ◽  
Ian W. Burton ◽  
John A. Walter ◽  
Pearse McCarron ◽  
...  
Keyword(s):  
Okadaic Acid ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Dsp Toxins
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