Expression pattern, subcellular localization, and functional implications of ODAM in ameloblasts, odontoblasts, osteoblasts, and various cancer cells

2012 ◽  
Vol 12 (3-4) ◽  
pp. 102-108 ◽  
Author(s):  
Hye-Kyung Lee ◽  
Su-Jin Park ◽  
Hyun-Jung Oh ◽  
Jung-Wook Kim ◽  
Hyun-Sook Bae ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Cancer Cells ◽  
Expression Pattern ◽  
Functional Implications

scholarly journals Functional Implications of Altered Subcellular Localization of PELP1 in Breast Cancer Cells

2005 ◽  
Vol 65 (17) ◽  
pp. 7724-7732 ◽  
Author(s):  
Ratna K. Vadlamudi ◽  
Bramanandam Manavathi ◽  
Seetharaman Balasenthil ◽  
Sujit S. Nair ◽  
Zhibo Yang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Subcellular Localization ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Functional Implications

Tu1920 Distinct Subcellular Localization of Phosphorylated ErbB2, ErbB3 and ErbB4 in Colorectal Cancer Cells and Their Relevance to Clinical Significance

2013 ◽  
Vol 144 (5) ◽  
pp. S-881
Author(s):  
Atsushi Tatsuguchi ◽  
Keigo Mitsui ◽  
Masaoki Yonezawa ◽  
Shu Tanaka ◽  
Shunji Fujimori ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Subcellular Localization ◽  
Cancer Cells ◽  
Clinical Significance ◽  
Colorectal Cancer Cells

scholarly journals Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 8165
Author(s):  
Amanda Chantziou ◽  
Kostas Theodorakis ◽  
Hara Polioudaki ◽  
Eelco de Bree ◽  
Marilena Kampa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Plasma Membrane ◽  
Subcellular Localization ◽  
Cancer Cells ◽  
Membrane Trafficking ◽  
Breast Cancer Cells ◽  
Endocytic Pathway ◽  
Cell Periphery ◽  
Wild Type ◽  
Mcf 7

In breast cancer, expression of Cluster of Differentiation 24 (CD24), a small GPI-anchored glycoprotein at the cell periphery, is associated with metastasis and immune escape, while its absence is associated with tumor-initiating capacity. Since the mechanism of CD24 sorting is unknown, we investigated the role of glycosylation in the subcellular localization of CD24. Expression and localization of wild type N36- and/or N52-mutated CD24 were analyzed using immunofluorescence in luminal (MCF-7) and basal B (MDA-MB-231 and Hs578T) breast cancer cells lines, as well as HEK293T cells. Endogenous and exogenously expressed wild type and mutated CD24 were found localized at the plasma membrane and the cytoplasm, but not the nucleoplasm. The cell lines showed different kinetics for the sorting of CD24 through the secretory/endocytic pathway. N-glycosylation, especially at N52, and its processing in the Golgi were critical for the sorting and expression of CD24 at the plasma membrane of HEK293T and basal B type cells, but not of MCF-7 cells. In conclusion, our study highlights the contribution of N-glycosylation for the subcellular localization of CD24. Aberrant N-glycosylation at N52 of CD24 could account for the lack of CD24 expression at the cell surface of basal B breast cancer cells.

scholarly journals Subcellular localization of FOXO3a as a potential biomarker of response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cancer cells

Oncotarget ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 6608-6622 ◽  
Author(s):  
Hyun-Jung Kim ◽  
Soo Yoon Lee ◽  
Chan Young Kim ◽  
Yun Hwan Kim ◽  
Woong Ju ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Cancer Cells ◽  
Combined Treatment ◽  
Potential Biomarker

scholarly journals APE1 and NPM1 protect cancer cells from platinum compounds cytotoxicity and their expression pattern has a prognostic value in TNBC

2019 ◽  
Vol 38 (1) ◽  
Author(s):  
Matilde Clarissa Malfatti ◽  
Lorenzo Gerratana ◽  
Emiliano Dalla ◽  
Miriam Isola ◽  
Giuseppe Damante ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Expression Pattern ◽  
Prognostic Value ◽  
Platinum Compounds

scholarly journals PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhengbin Chai ◽  
Li Wang ◽  
Yabing Zheng ◽  
Na Liang ◽  
Xiwei Wang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Molecular Mechanism ◽  
Expression Pattern ◽  
Colony Formation ◽  
Regulatory Mechanism ◽  
Cancer Cell Proliferation ◽  
Colon Cancer Cells ◽  
Cancer Tissues

Abstract Background CKS1 is highly expressed in colon cancer tissues, and is essential for cancer cell proliferation. The downstream molecular mechanism of CKS1 has been fully studied, but the upstream regulatory mechanism of it is still unclear. Earlier research found that PADI3 plays its anti-tumor roles via suppress cell proliferation, in this study, we found that the expression pattern of PADI3 and CKS1 are negatively correlated in colon cancer tissues, and overexpression of PADI3 can partly reverse CKS1 induced cancer cell proliferation. However, the regulatory mechanism of PADI3 and CKS1 in the tumorigenesis of colon cancer is still unclear and need to do further research. Methods Western blot and real-time PCR were used to detect the expression levels of genes. CCK-8 and colony formation assays were used to examine cell proliferation and colony formation ability. Overexpression and rescue experiments were used to study the molecular mechanism of CKS1 in colon cancer cells, BALB/c nude mice were used to study the function of CKS1 in vivo. Results CKS1 is highly expressed in colon cancer tissues, and the overexpression of CKS1 promotes cell proliferation and colony formation in both HCT116 (originating from primary colon cancer) and SW620 (originating from metastatic tumor nodules of colon cancer) cells. CKS1-expressing HCT116 cells produced larger tumors than the control cells. The expression pattern of PADI3 and CKS1 are negatively correlation in clinical samples of colon cancer, further study indicates that PADI3 can significantly decrease Hsp90 and CKS1 expression, and Hsp90 is essential for PADI3 to downregulate CKS1expression in colon cancer cells. Conclusions PADI3 exerts its antitumor activity by inhibiting Hsp90 and CKS1 expression, and Hsp90 is essential for PADI3 to suppress CKS1 expression.

Sign in / Sign up

Export Citation Format

Share Document