Molecular characterization of Hyalomma dromedarii from North Western Region of India based on the gene sequences encoding Calreticulin and Internally Transcribed Spacer Region 2

Gene Reports ◽  
2018 ◽  
Vol 10 ◽  
pp. 141-148 ◽  
Author(s):  
G. Sivakumar ◽  
Shelesh Kumar Swami ◽  
Govindasamy Nagarajan ◽  
S.C. Mehta ◽  
F.C. Tuteja ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Western Region ◽  
Gene Sequences ◽  
Hyalomma Dromedarii ◽  
Internally Transcribed Spacer ◽  
North Western

scholarly journals Molecular Characterization of anEndozoicomonas-Like Organism Causing Infection in the King Scallop (Pecten maximusL.)

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Irene Cano ◽  
Ronny van Aerle ◽  
Stuart Ross ◽  
David W. Verner-Jeffreys ◽  
Richard K. Paley ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Mass Mortality ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
The 16S Rrna Gene

ABSTRACTOne of the fastest growing fisheries in the UK is the king scallop (Pecten maximusL.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay marine protected area (MPA) in Southwest England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies (IMCs) of bacteria resemblingRickettsia-like organisms (RLOs), often with bacteria released in vascular spaces. Large colonies were associated with cellular and tissue disruption of the gills. Ultrastructural examination confirmed the intracellular location of these organisms in affected epithelial cells. The 16S rRNA gene sequences of the putative IMCs obtained from infected king scallop gill samples, collected from both mortality events, were identical and had a 99.4% identity to 16S rRNA gene sequences obtained from “CandidatusEndonucleobacter bathymodioli” and 95% withEndozoicomonasspecies.In situhybridization assays using 16S rRNA gene probes confirmed the presence of the sequenced IMC gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing, and bioinformatic analysis identified over 1,000 genes with high similarity to protein sequences fromEndozoicomonasspp. (ranging from 77 to 87% identity). Specific PCR assays were developed and applied to screen for the presence of IMC 16S rRNA gene sequences in king scallop gill tissues collected at the Lyme Bay MPA during 2015 and 2016. There was 100% prevalence of the IMCs in these gill tissues, and the 16S rRNA gene sequences identified were identical to the sequence found during the previous mortality event.IMPORTANCEMolluscan mass mortalities associated with IMCs have been reported worldwide for many years; however, apart from histological and ultrastructural characterization, characterization of the etiological agents is limited. In the present work, we provide detailed molecular characterization of anEndozoicomonas-like organism (ELO) associated with an important commercial scallop species.

Identification and Molecular Characterization of ‘CandidatusPhytoplasma mali’ Isolates in North-western Italy

2010 ◽  
Vol 158 (2) ◽  
pp. 81-87 ◽  
Author(s):  
Paola Casati ◽  
Fabio Quaglino ◽  
Rosemarie Tedeschi ◽  
Fabio Mario Spiga ◽  
Alberto Alma ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
North Western

Epidemiology of ticks and molecular characterization of Rhipicephalus microplus in cattle population in North-Western Pakistan

2020 ◽  
Vol 46 (5) ◽  
pp. 335-343
Author(s):  
Jehan Zeb ◽  
Sumaira Shams ◽  
Sultan Ayaz ◽  
Israr Ud Din ◽  
Adil Khan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rhipicephalus Microplus ◽  
Cattle Population ◽  
North Western

scholarly journals Molecular Characterization of Hyalomma dromedarii from Bikaner, India

2014 ◽  
Vol V (10) ◽  
pp. 52-58 ◽  
Author(s):  
HARISH K. CH CHANGAL ◽  
G. N NAGARAJAN ◽  
R. K PUROHIT ◽  
SHELESH KUMAR SWAMI ◽  
S.C. MEH MEHTA ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hyalomma Dromedarii

scholarly journals Molecular Characterization of Cleidocranial Dysplasia (CCD) in the Western Region in KSA

2017 ◽  
Vol 6 (4) ◽  
pp. 1373-1385
Author(s):  
Adel E. El Tarras Mohammed A. El Awady ◽  
Samer, E. M. Ismail Naglaa M. Kamal ◽  
Adnan A. Amin
Keyword(s):  
Molecular Characterization ◽  
Cleidocranial Dysplasia ◽  
Western Region ◽  
The Western Region

Prevalence and molecular characterization of Rickettsia spp. in questing ticks from north-western Spain

2019 ◽  
Vol 79 (2) ◽  
pp. 267-278 ◽  
Author(s):  
Susana Remesar ◽  
Pablo Díaz ◽  
Aránzazu Portillo ◽  
Sonia Santibáñez ◽  
Alberto Prieto ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Questing Ticks ◽  
Western Spain ◽  
North Western

Molecular characterization of an indigenous minor carp Bangana dero (Cyprinidae: Labeoninae) of Manipur and its relationship with Labeonin fishes of North East India based on mitochondrial 16SrRNA gene sequences

2018 ◽  
Author(s):  
Ch. Basudha ◽  
N. Sobita ◽  
A. Rawat and N. Prakash
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Standard Length ◽  
Cyprinid Fish ◽  
Gene Sequences ◽  
North East India ◽  
Cyprinid Species ◽  
North East ◽  
16Srrna Gene

Bangana dero is an economically important indigenous cyprinid fish of Manipur, India having maximum standard length of 40cm. For the taxonomic placement of this species among the Labeonin fishes, molecular characterization is conducted by using mitochondrial 16SrRNA gene sequences. Partial sequences of mito. 16SrRNA gene of fifteen cyprinid species of the sub-family Labeoninae were downloaded from NCBI Genbank. The analysed nucleotide sequence lengths were 538 bp. In a total of 811 characters, 194 were conserved sites (monomorphic) and 347 were variable sites (polymorphic) and out of 347 variable sites, 73 were parsimony informative sites and 274 singleton sites. The nucleotide frequencies are 31.4% (A), 22.57% (T), 24.00% (C) and 21.96% (G). The transition/transversion bias (R) is 2.51. The overall mean distance is 0.083. The dendogram constructed by Neighbour-Joining tree have three clusters which can indicate the taxonomic positions of Bangana dero .

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