scholarly journals Aqueous areca nut extract induces oxidative stress in human lung epithelial A549 cells: Probable role of p21 in inducing cell death

Gene Reports ◽  
2017 ◽  
Vol 6 ◽  
pp. 103-111 ◽  
Author(s):  
Rashmi Nagesh ◽  
K. M Kiran Kumar ◽  
M Naveen Kumar ◽  
Rajeshwari H. Patil ◽  
K Kavya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Human Lung ◽  
A549 Cells ◽  
Areca Nut ◽  
Lung Epithelial ◽  
Probable Role

scholarly journals Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells

Toxics ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 78
Author(s):  
Naoki Endo ◽  
Takashi Toyama ◽  
Akira Naganuma ◽  
Yoshiro Saito ◽  
Gi-Wook Hwang
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Death ◽  
Human Lung ◽  
A549 Cells ◽  
Protein Levels ◽  
Intracellular Ros ◽  
Lung Epithelial ◽  
Homeobox Protein

Although homeobox protein B13 (HOXB13) is an oncogenic transcription factor, its role in stress response has rarely been examined. We previously reported that knockdown of HOXB13 reduces the cytotoxicity caused by various oxidative stress inducers. Here, we studied the role of HOXB13 in cytotoxicity caused by hydrogen peroxide in human lung epithelial A549 cells. The knockdown of HOXB13 reduced hydrogen peroxide-induced cytotoxicity; however, this phenomenon was largely absent in the presence of antioxidants (Trolox or N-acetyl cysteine (NAC)). This suggests that HOXB13 may be involved in the cytotoxicity caused by hydrogen peroxide via the production of reactive oxygen species (ROS). Hydrogen peroxide also increased both the mRNA and protein levels of HOXB13. However, these increases were rarely observed in the presence of a transcriptional inhibitor, which suggests that hydrogen peroxide increases protein levels via increased transcription of HOXB13. Furthermore, cell death occurred in A549 cells that highly expressed HOXB13. However, this cell death was mostly inhibited by treatment with antioxidants. Taken together, our findings indicate that HOXB13 may be a novel factor involved in the induction of oxidative stress, which causes cell death via intracellular ROS production.

scholarly journals Role of the MAPK pathway in human lung epithelial-like A549 cells apoptosis induced by paraquat

2020 ◽  
Vol 43 (2) ◽  
Author(s):  
Da-Zhuang Sun ◽  
Chun-Qing Song ◽  
Yong-Min Xu ◽  
Xue-Song Dong
Keyword(s):  
Human Lung ◽  
Mapk Pathway ◽  
A549 Cells ◽  
Lung Epithelial

scholarly journals Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Nadia Ferlazzo ◽  
Giuseppa Visalli ◽  
Antonella Smeriglio ◽  
Santa Cirmi ◽  
Giovanni Enrico Lombardo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Injury ◽  
Human Lung ◽  
Antioxidant Properties ◽  
A549 Cells ◽  
Lung Epithelial Cells ◽  
Food Sources ◽  
Plant Metabolites ◽  
Lung Epithelial ◽  
Flavonoid Fraction

It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, andCitrusfruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that bothCitrusjuice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

Oxidative stress-induced regulation of the methionine metabolic pathway in human lung epithelial-like (A549) cells

2009 ◽  
Vol 674 (1-2) ◽  
pp. 23-30 ◽  
Author(s):  
Mihalis I. Panayiotidis ◽  
Sally P. Stabler ◽  
Robert H. Allen ◽  
Aglaia Pappa ◽  
Carl W. White
Keyword(s):  
Oxidative Stress ◽  
Metabolic Pathway ◽  
Human Lung ◽  
A549 Cells ◽  
Lung Epithelial

scholarly journals Induction of Apoptotic Cell Death by Synthetic Naringenin Derivatives in Human Lung Epithelial Carcinoma A549 Cells

2007 ◽  
Vol 30 (12) ◽  
pp. 2394-2398 ◽  
Author(s):  
Eung-Ryoung Lee ◽  
Yong-Jin Kang ◽  
Hye-Yeon Choi ◽  
Geun-Ho Kang ◽  
Jung-Hyun Kim ◽  
...  
Keyword(s):  
Cell Death ◽  
Human Lung ◽  
Apoptotic Cell ◽  
A549 Cells ◽  
Apoptotic Cell Death ◽  
Lung Epithelial
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