scholarly journals Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU)

Genomics Data ◽  
2015 ◽  
Vol 5 ◽  
pp. 64-67 ◽  
Author(s):  
Francesco Chemello ◽  
Cristina Mammucari ◽  
Gaia Gherardi ◽  
Rosario Rizzuto ◽  
Gerolamo Lanfranchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Calcium Uniporter ◽  
Skeletal Muscle Fibers

scholarly journals Repression of slow myosin heavy chain 2 gene expression in fast skeletal muscle fibers by muscarinic acetylcholine receptor and Gαq signaling

2003 ◽  
Vol 162 (5) ◽  
pp. 843-850 ◽  
Author(s):  
Theresa Jordan ◽  
Jinyuan Li ◽  
Hongbin Jiang ◽  
Joseph X. DiMario
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Muscle Fibers ◽  
Muscarinic Acetylcholine Receptor ◽  
Fast Skeletal Muscle ◽  
Muscarinic Acetylcholine ◽  
Fiber Properties ◽  
Skeletal Muscle Fibers ◽  
Slow Myosin Heavy Chain

Gene expression in skeletal muscle fibers is regulated by innervation and intrinsic fiber properties. To determine the mechanism of repression of slow MyHC2 expression in innervated fast pectoralis major (PM) fibers, we investigated the function of the muscarinic acetylcholine receptor (mAchR) and Gαq. Both mAchR and Gαq are abundant in medial adductor (MA) and PM fibers, and mAchR and Gαq interact in these fibers. Whereas innervation of PM fibers was insufficient to induce slow MyHC2 expression, inhibition of mAchR activity with atropine in innervated PM fibers induced slow MyHC2 expression. Increased Gαq activity repressed slow MyHC2 expression to nondetectable levels in innervated MA fibers. Reduced mAchR activity decreased PKC activity in PM fibers, and increased Gαq activity increased PKC activity in PM and MA fibers. Decreased PKC activity in atropine-treated innervated PM fibers correlated with slow MyHC2 expression. These data suggest that slow MyHC2 repression in innervated fast PM fibers is mediated by cell signaling involving mAchRs, Gαq, and PKC.

scholarly journals The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle

JCI Insight ◽  
2018 ◽  
Vol 3 (22) ◽  
Author(s):  
Jennifer Q. Kwong ◽  
Jiuzhou Huo ◽  
Michael J. Bround ◽  
Justin G. Boyer ◽  
Jennifer A. Schwanekamp ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Metabolic Fuel ◽  
Calcium Uniporter ◽  
Fuel Preference

scholarly journals Priority Strategy of Intracellular Ca2+ Homeostasis in Skeletal Muscle Fibers during the Multiple Stresses of Hibernation

Cells ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 42 ◽  
Author(s):  
Jie Zhang ◽  
Xiaoyu Li ◽  
Fazeela Ismail ◽  
Shenhui Xu ◽  
Zhe Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Leucine Zipper ◽  
Binding Capacity ◽  
Transmembrane Protein ◽  
Muscle Fibers ◽  
Vital Role ◽  
Ground Squirrels ◽  
Calcium Uniporter ◽  
Skeletal Muscle Fibers ◽  
Ef Hand

Intracellular calcium (Ca2+) homeostasis plays a vital role in the preservation of skeletal muscle. In view of the well-maintained skeletal muscle found in Daurian ground squirrels (Spermophilus dauricus) during hibernation, we hypothesized that hibernators possess unique strategies of intracellular Ca2+ homeostasis. Here, cytoplasmic, sarcoplasmic reticulum (SR), and mitochondrial Ca2+ levels, as well as the potential Ca2+ regulatory mechanisms, were investigated in skeletal muscle fibers of Daurian ground squirrels at different stages of hibernation. The results showed that cytoplasmic Ca2+ levels increased in the skeletal muscle fibers during late torpor (LT) and inter-bout arousal (IBA), and partially recovered when the animals re-entered torpor (early torpor, ET). Furthermore, compared with levels in the summer active or pre-hibernation state, the activity and protein expression levels of six major Ca2+ channels/proteins were up-regulated during hibernation, including the store-operated Ca2+ entry (SOCE), ryanodine receptor 1 (RyR1), leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), SR Ca2+ ATPase 1 (SERCA1), mitochondrial calcium uniporter complex (MCU complex), and calmodulin (CALM). Among these, the increased extracellular Ca2+ influx mediated by SOCE, SR Ca2+ release mediated by RyR1, and mitochondrial Ca2+ extrusion mediated by LETM1 may be triggers for the periodic elevation in cytoplasmic Ca2+ levels observed during hibernation. Furthermore, the increased SR Ca2+ uptake through SERCA1, mitochondrial Ca2+ uptake induced by MCU, and elevated free Ca2+ binding capacity mediated by CALM may be vital strategies in hibernating ground squirrels to attenuate cytoplasmic Ca2+ levels and restore Ca2+ homeostasis during hibernation. Compared with that in LT or IBA, the decreased extracellular Ca2+ influx mediated by SOCE and elevated mitochondrial Ca2+ uptake induced by MCU may be important mechanisms for the partial cytoplasmic Ca2+ recovery in ET. Overall, under extreme conditions, hibernating ground squirrels still possess the ability to maintain intracellular Ca2+ homeostasis.

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