scholarly journals Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer

Genomics Data ◽  
2015 ◽  
Vol 4 ◽  
pp. 119-122 ◽  
Author(s):  
Nicholas Borcherding ◽  
Nicholas Bormann ◽  
David Kusner ◽  
Ryan Kolb ◽  
Weizhou Zhang
Keyword(s):  
Breast Cancer ◽  
Transcriptome Analysis ◽  
Tumor Initiating Cells ◽  
Luminal Tumor

Antitumor Activity and Underlying Mechanism of Phomoxanthone B in MCF7 Cells

2020 ◽  
Vol 20 ◽  
Author(s):  
Chuan Chen ◽  
Ziyue Zhao ◽  
Qian Dong ◽  
XueHui Gao ◽  
Huibin Xu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Antitumor Activity ◽  
Oxidative Phosphorylation ◽  
Tumor Cells ◽  
Transcriptome Analysis ◽  
Mcf7 Cells ◽  
Er Positive ◽  
Induced Apoptosis ◽  
Positive Breast Cancer

Background:: Xanthones are a class of heterocyclic natural products, which are promising sources of anticancer leads. Phomoxanthone B（PXB）and Phomoxanthone A（PXA）are xanthone dimers. PXA is well studied as an anti-cancer agent, but PXB is not. In our study, PXB was isolated from the endophytic fungus Phomopsis sp. By254. Objective:: The purpose of this study was to identify the underlying anti-tumor mechanisms of PXB in breast cancer MCF7 cell line. Methods:: Apoptosis, cell cycle, proliferation, invasion and migration assays were used to assess the antitumor activity of PXB. RNA sequencing was used to analyze the effect of PXB treatment on gene expression in MCF7 cells. Results:: PXB showed cytotoxicity toward a variety of tumor cells, especially MCF7 cells. PXB inhibited the migration and invasion, arrested cell cycle at G2/M phase and induced apoptosis associated with caspase-3 activation in MCF7 cells. The detailed transcriptome analysis revealed that PXB affected several pathways related to tumorigenesis, metabolisms-, and oxidative phosphorylation in MCF7 cells. KEGG transcriptome analysis revealed that PXB upregulated pro-survival signal pathways such as MAPK, PI3K-AKT and STAT3 pathways. We found that PXB also significantly upregulated the expression of IL24, DDIT3 and XAF1, which may contribute to PXB-induced apoptosis. We further found that PXB may downregulate oxidative phosphorylation by decreasing the expression of electron transport chain genes, especially MT-ND1, which is a potential unfavorable prognostic marker for ER-positive breast cancer. Conclusion:: PXB exerts strong cytotoxicity against human tumor cells and has a potential for ER-positive breast cancer treatment.

scholarly journals TEM8 marks neovasculogenic tumor-initiating cells in triple-negative breast cancer

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiahui Xu ◽  
Xiaoli Yang ◽  
Qiaodan Deng ◽  
Cong Yang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Breast Tumor ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Vasculogenic Mimicry ◽  
Regulatory Mechanisms ◽  
Tumor Initiating Cells ◽  
Self Renewal ◽  
Tumor Endothelial Marker 8

AbstractEnhanced neovasculogenesis, especially vasculogenic mimicry (VM), contributes to the development of triple-negative breast cancer (TNBC). Breast tumor-initiating cells (BTICs) are involved in forming VM; however, the specific VM-forming BTIC population and the regulatory mechanisms remain undefined. We find that tumor endothelial marker 8 (TEM8) is abundantly expressed in TNBC and serves as a marker for VM-forming BTICs. Mechanistically, TEM8 increases active RhoC level and induces ROCK1-mediated phosphorylation of SMAD5, in a cascade essential for promoting stemness and VM capacity of breast cancer cells. ASB10, an estrogen receptor ERα trans-activated E3 ligase, ubiquitylates TEM8 for degradation, and its deficiency in TNBC resulted in a high homeostatic level of TEM8. In this work, we identify TEM8 as a functional marker for VM-forming BTICs in TNBC, providing a target for the development of effective therapies against TNBC targeting both BTIC self-renewal and neovasculogenesis simultaneously.

scholarly journals The role of steroid hormones in breast cancer stem cells

2015 ◽  
Vol 22 (6) ◽  
pp. T177-T186 ◽  
Author(s):  
Bruno M Simões ◽  
Denis G Alferez ◽  
Sacha J Howell ◽  
Robert B Clarke
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Steroid Hormones ◽  
Normal Mammary Gland ◽  
Breast Cancer Stem Cells ◽  
Tumor Initiating Cells ◽  
Unit Of Selection ◽  
Selection For

Breast cancer stem cells (BCSCs) are potent tumor-initiating cells in breast cancer, the most common cancer among women. BCSCs have been suggested to play a key role in tumor initiation which can lead to disease progression and formation of metastases. Moreover, BCSCs are thought to be the unit of selection for therapy-resistant clones since they survive conventional treatments, such as chemotherapy, irradiation, and hormonal therapy. The importance of the role of hormones for both normal mammary gland and breast cancer development is well established, but it was not until recently that the effects of hormones on BCSCs have been investigated. This review will discuss recent studies highlighting how ovarian steroid hormones estrogen and progesterone, as well as therapies against them, can regulate BCSC activity.

scholarly journals Small Molecule Antagonists of the Wnt/Beta-Catenin Signaling Pathway Target Breast Tumor-Initiating Cells in a Her2/Neu Mouse Model of Breast Cancer

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33976 ◽  
Author(s):  
Robin M. Hallett ◽  
Maria K. Kondratyev ◽  
Andrew O. Giacomelli ◽  
Allison M. L. Nixon ◽  
Adele Girgis-Gabardo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mouse Model ◽  
Signaling Pathway ◽  
Small Molecule ◽  
Breast Tumor ◽  
Beta Catenin ◽  
Tumor Initiating Cells

scholarly journals Selective Small Molecule Stat3 Inhibitor Reduces Breast Cancer Tumor-Initiating Cells and Improves Recurrence Free Survival in a Human-Xenograft Model

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e30207 ◽  
Author(s):  
Bhuvanesh Dave ◽  
Melissa D. Landis ◽  
Lacey E. Dobrolecki ◽  
Meng-Fen Wu ◽  
Xiaomei Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Small Molecule ◽  
Xenograft Model ◽  
Recurrence Free Survival ◽  
Tumor Initiating Cells ◽  
Free Survival ◽  
Cancer Tumor

scholarly journals Single cell transcriptome analysis upon MCF-7 breast cancer

2010 ◽  
Vol 11 (Suppl 1) ◽  
pp. P8 ◽  
Author(s):  
Yih-Shien Chiang ◽  
Lock Seng ◽  
You-Yu Lin ◽  
Shih-Hao Chen ◽  
Yu-Chang Su ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome ◽  
Mcf 7
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