scholarly journals Transcriptome profiling for discovery of genes involved in shoot apical meristem and flower development

Genomics Data ◽  
2014 ◽  
Vol 2 ◽  
pp. 135-138 ◽  
Author(s):  
Vikash K. Singh ◽  
Mukesh Jain
Keyword(s):  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Transcriptome Profiling

scholarly journals FAR-RED ELONGATED HYPOCOTYL3 activates SEPALLATA2 but inhibits CLAVATA3 to regulate meristem determinacy and maintenance in Arabidopsis

2016 ◽  
Vol 113 (33) ◽  
pp. 9375-9380 ◽  
Author(s):  
Dongming Li ◽  
Xing Fu ◽  
Lin Guo ◽  
Zhigang Huang ◽  
Yongpeng Li ◽  
...  
Keyword(s):  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Target Genes ◽  
Developmental Stages ◽  
Binding Motif ◽  
Phytochrome B ◽  
Helix Loop Helix ◽  
Before And After ◽  
Meristem Maintenance

Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the establishment and maintenance of meristems, are epistatic to FHY3. Using genome-wide ChIP-seq and RNA-seq data, we identify hundreds of FHY3 target genes in flowers and find that FHY3 mainly acts as a transcriptional repressor in flower development, in contrast to its transcriptional activator role in seedlings. Binding motif-enrichment analyses indicate that FHY3 may coregulate flower development with three flower-specific MADS-domain TFs and four basic helix–loop–helix TFs that are involved in photomorphogenesis. We further demonstrate that CLV3, SEPALLATA1 (SEP1), and SEP2 are FHY3 target genes. In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool. Intriguingly, CLV3 expression did not change significantly in fhy3 and phytochrome B mutants before and after light treatment, indicating that FHY3 and phytochrome B are involved in light-regulated meristem activity. In FM, FHY3 directly represses CLV3, but activates SEP2, to ultimately promote FM determinacy. Taken together, our results reveal insights into the mechanisms of meristem maintenance and determinacy, and illustrate how the roles of a single TF may vary in different organs and developmental stages.

scholarly journals HANABA TARANU Is a GATA Transcription Factor That Regulates Shoot Apical Meristem and Flower Development in Arabidopsis

2004 ◽  
Vol 16 (10) ◽  
pp. 2586-2600 ◽  
Author(s):  
Yuanxiang Zhao ◽  
Leonard Medrano ◽  
Kazuaki Ohashi ◽  
Jennifer C. Fletcher ◽  
Hao Yu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Gata Transcription Factor

Flower meristem maintenance by TILLERS ABSENT 1 is essential for ovule development in rice

Development ◽  
2021 ◽  
Vol 148 (24) ◽  
Author(s):  
Wakana Tanaka ◽  
Suzuha Ohmori ◽  
Naoto Kawakami ◽  
Hiro-Yuki Hirano
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Stem Cell Marker ◽  
Ovule Development ◽  
Flower Meristem ◽  
Ovule Formation ◽  
Meristem Maintenance

ABSTRACT Plant development depends on the activity of pluripotent stem cells in meristems, such as the shoot apical meristem and the flower meristem. In Arabidopsis thaliana, WUSCHEL (WUS) is essential for stem cell homeostasis in meristems and integument differentiation in ovule development. In rice (Oryza sativa), the WUS ortholog TILLERS ABSENT 1 (TAB1) promotes stem cell fate in axillary meristem development, but its function is unrelated to shoot apical meristem maintenance in vegetative development. In this study, we examined the role of TAB1 in flower development. The ovule, which originates directly from the flower meristem, failed to differentiate in tab1 mutants, suggesting that TAB1 is required for ovule formation. Expression of a stem cell marker was completely absent in the flower meristem at the ovule initiation stage, indicating that TAB1 is essential for stem cell maintenance in the ‘final’ flower meristem. The ovule defect in tab1 was partially rescued by floral organ number 2 mutation, which causes overproliferation of stem cells. Collectively, it is likely that TAB1 promotes ovule formation by maintaining stem cells at a later stage of flower development.

scholarly journals Sample preparation for laser-microdissection of soybean shoot apical meristem

2012 ◽  
Vol 3 (1) ◽  
pp. 3 ◽  
Author(s):  
Chui E. Wong ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Sample Preparation ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Laser Microdissection ◽  
Specific Cell ◽  
Legume Crop ◽  
Continuous Formation ◽  
Molecular Events ◽  
Depth Analysis ◽  
Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

Planta ◽  
2002 ◽  
Vol 214 (6) ◽  
pp. 829-836 ◽  
Author(s):  
Andreas Mordhorst ◽  
Marijke Hartog ◽  
Mazen El Tamer ◽  
Thomas Laux ◽  
Sacco de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Apical Meristem ◽  
Apical Meristem

scholarly journals Arabidopsis Argonaute10 Specifically Sequesters miR166/165 to Regulate Shoot Apical Meristem Development

Cell ◽  
2011 ◽  
Vol 145 (2) ◽  
pp. 242-256 ◽  
Author(s):  
Hongliang Zhu ◽  
Fuqu Hu ◽  
Ronghui Wang ◽  
Xin Zhou ◽  
Sing-Hoi Sze ◽  
...  
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Development

scholarly journals Spatiotemporal Sequestration of miR165/166 by Arabidopsis Argonaute10 Promotes Shoot Apical Meristem Maintenance

Cell Reports ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. 1819-1827 ◽  
Author(s):  
Yuyi Zhou ◽  
Minami Honda ◽  
Hongliang Zhu ◽  
Zhonghui Zhang ◽  
Xinwei Guo ◽  
...  
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Maintenance

Phyllotaxis of the palm Euterpe oleracea Mart. at the level of the shoot apical meristem

Botany ◽  
2010 ◽  
Vol 88 (5) ◽  
pp. 528-536 ◽  
Author(s):  
Denis Barabé ◽  
Laura Bourque ◽  
Xiaofeng Yin ◽  
Christian Lacroix
Keyword(s):  
Shoot Apical Meristem ◽  
Fundamental Theorem ◽  
Apical Meristem ◽  
Leaf Primordia ◽  
Leaf Primordium ◽  
Divergence Angle ◽  
Euterpe Oleracea ◽  
The Mean ◽  
The Relationship ◽  
Euterpe Oleracea Mart

Previous studies on palm phyllotaxis deal mainly with the mature trunk. The goals of this study are (i) to determine the relationship between the number of parastichies, the divergence angle, and the plastochrone ratio at the level of the shoot apical meristem; (ii) to examine whether there are fluctuations in the divergence angle; (iii) to interpret the significance of phyllotactic parameters with respect to the mode of growth of the apex. The tubular base of the leaf primordium is more or less asymmetrical, and completely surrounds the shoot apical meristem. The phyllotactic system corresponds to a (2, 3) conspicuous parastichy pair. The mean divergence angle per apex varies between 126.9° ± 9.3° (mean ± SD) and 135. 8° ± 8.0°. Divergence angles for all apices fluctuate within a range of 115.89° to 157.33°. The mean plastochrone ratios between apices varies from 1.35 ± 0.18 to 1.58 ± 0.12. The plastochrone ratio at each plastochrone for all apices ranges from 1.09 to 2.00. There is no correlation between the angle of divergence and the plastochrone ratio. There is a fluctuation in the value of the divergence angle that falls within the range predicted by the fundamental theorem of phyllotaxis. The high value of the ratio of the diameter of leaf primordia over the diameter of the apex, and the long plastochrone might explain the lack of correlation between certain phyllotactic parameters.

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