scholarly journals Concordance and reproducibility of a next generation mtGenome sequencing method for high-quality samples using the Illumina MiSeq

2016 ◽  
Vol 24 ◽  
pp. 103-111 ◽  
Author(s):  
Michelle A. Peck ◽  
Michael D. Brandhagen ◽  
Charla Marshall ◽  
Toni M. Diegoli ◽  
Jodi A. Irwin ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Next Generation ◽  
High Quality ◽  
Sequencing Method

Developmental validation of a Nextera XT mitogenome Illumina MiSeq sequencing method for high-quality samples

2018 ◽  
Vol 34 ◽  
pp. 25-36 ◽  
Author(s):  
Michelle A. Peck ◽  
Kimberly Sturk-Andreaggi ◽  
Jacqueline T. Thomas ◽  
Robert S. Oliver ◽  
Suzanne Barritt-Ross ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Illumina Miseq Sequencing ◽  
Miseq Sequencing ◽  
High Quality ◽  
Sequencing Method ◽  
Developmental Validation

Development of High-Quality Newsprint for Next Generation

2006 ◽  
Vol 60 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Fuminari Nonomura
Keyword(s):  
Next Generation ◽  
High Quality

scholarly journals Semiconductor Heteroepitaxy

Crystals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 229
Author(s):  
Roberto Bergamaschini ◽  
Elisa Vitiello
Keyword(s):  
High Performance ◽  
Next Generation ◽  
High Quality

The quest for high-performance and scalable devices required for next-generation semiconductor applications inevitably passes through the fabrication of high-quality materials and complex designs [...]

scholarly journals Development of a Next-Generation Sequencing Method for BRCA Mutation Screening

2012 ◽  
Vol 14 (6) ◽  
pp. 602-612 ◽  
Author(s):  
Maurice Chan ◽  
Shen Mo Ji ◽  
Zhen Xuan Yeo ◽  
Linda Gan ◽  
Eric Yap ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Brca Mutation ◽  
Mutation Screening ◽  
Next Generation ◽  
Sequencing Method ◽  
Generation Sequencing ◽  
Next Generation Sequencing Method

Materials with Dirac Loops: A Potential Solution for Next-generation Electronics

2021 ◽  
Vol 1 ◽  
Keyword(s):  
Electronic Structure ◽  
Single Crystal ◽  
Potential Solution ◽  
Next Generation ◽  
Rhenium Oxide ◽  
High Quality ◽  
High Quality Single Crystal

A high-quality single crystal of rhenium oxide shows significantly large magnetoresistance, potentially originating from a unique electronic structure called “hourglass Dirac chain” protected by the symmetry of the crystal.

scholarly journals 1337. Development, Maintenance, and Application of Opsonophagocytic Assays to Measure Functional Antibody Responses to Support a 20 Valent Pneumococcal Conjugate Vaccine

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S484-S484
Author(s):  
Ingrid L Scully ◽  
Mark W Cutler ◽  
Seema Gangolli ◽  
Todd Belanger ◽  
David Cooper ◽  
...  
Keyword(s):  
Quality Control ◽  
Antibody Responses ◽  
Antibody Activity ◽  
Pneumococcal Vaccines ◽  
Next Generation ◽  
High Quality ◽  
Assay Performance ◽  
Control Serum ◽  
Serological Data ◽  
Over Time

Abstract Background Opsonophagocytic assays (OPAs) are an important tool for assessing vaccine-induced functional antibody responses. OPAs are complex assays composed of many biological components (eg serum, complement sources, bacteria, and human phagocytes) which contribute to assay variability and may result in titer drift if not carefully controlled. Rigorous development and validation coupled with routine monitoring of assay performance are required to ensure that high-quality OPA serological data are consistently generated throughout the lifetime of existing and next-generation pneumococcal vaccines. Methods OPA specificity was demonstrated by competing functional antibody activity with pneumococcal polysaccharides. Assay qualification/validation assessed accuracy, precision, and sample linearity. Assay performance over time was assessed through the implementation of quality control serum data tracking systems and longterm serum proficiency panels that are routinely tested during assay performance. Human quality control sera are included on each assay plate to ensure that each plate meets pre-specified acceptance criteria. Proficiency serum panels are comprised of individual human serum samples derived from subjects immunized with pneumococcal vaccines and are used to monitor performance across a range of serological titers and over time. Results The OPAs were shown to be specific and reproducible. Monitoring of assay performance over time demonstrated that the assays are stable. For the 13 serotypes contained in 13vPnC reliable titers have been generated in over a decade of testing which is an essential prerequisite in the evaluation of next-generation pneumococcal conjugate vaccines such as 20vPnC, whose licensure depends on demonstration of non-inferiority to 13vPnC. Conclusion Maintenance and careful monitoring of high-quality assays to measure functional antibody responses, such as OPAs, is critical for the delivery of reliable serological data to support the advancement of pneumococcal vaccine programs. Pneumococcal OPAs must be rigorously maintained to ensure continuity of serological data over time and inform licensure decisions of next-generation vaccines as well as postmarketing and seroepidemiology studies. Disclosures All authors: No reported disclosures.

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