scholarly journals Facile semi-automated forensic body fluid identification by multiplex solution hybridization of NanoString® barcode probes to specific mRNA targets

2015 ◽  
Vol 14 ◽  
pp. 18-30 ◽  
Author(s):  
Patrick Danaher ◽  
Robin Lynn White ◽  
Erin K. Hanson ◽  
Jack Ballantyne
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Targets ◽  
Specific Mrna

scholarly journals Facile semi-automated forensic body fluid identification by multiplex solution hybridization of NanoString® barcode probes to specific mRNA targets

2014 ◽  
Author(s):  
Patrick Danaher ◽  
Robin Lynn White ◽  
Erin Hanson ◽  
Jack Ballantyne
Keyword(s):  
Gene Expression ◽  
Body Fluid ◽  
Body Fluids ◽  
Menstrual Blood ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Profiling ◽  
Mrna Targets ◽  
Vaginal Secretions ◽  
Specific Mrna

A DNA profile from the perpetrator does not reveal, per se, the circumstances by which it was transferred. Body fluid identification by mRNA profiling may allow extraction of contextual 'activity level' information from forensic samples. Here we describe the development of a prototype multiplex digital gene expression (DGE) method for forensic body fluid/tissue identification based upon solution hybridization of color-coded NanoString® probes to 23 tissue/body fluid specific mRNA targets. The body fluids/tissues targeted were peripheral blood, semen, saliva, vaginal secretions, menstrual blood and skin. We tested and compared a simple 5 minute room temperature cellular lysis protocol against standard RNA isolation from same source material as a means to facilitate ease-of-use in forensic sample processing. We first describe a model for gene expression in a sample from a single body fluid and then extend that model to mixtures of body fluids. We then describe calculation of maximum likelihood estimates (MLEs) of body fluid quantities in a sample, and we describe the use of likelihood ratios to test for the presence of each body fluid in a sample. Known single source blood, semen, vaginal secretions, menstrual blood and skin samples all demonstrated the expected tissue specific gene expression for at least two of the chosen biomarkers. Saliva samples were more problematic, with their previously identified characteristic genes exhibiting poor specificity. Nonetheless the most specific saliva biomarker, HTN3, was expressed at a higher level in saliva than in any of the other tissues. As a preliminary indication of the ability of the method to discern admixtures of body fluids, five mixtures were prepared. Two of the five mixtures were called perfectly using the assay algorithm, and one of the component fluids was identified in the each of the 'false negative' mixtures. Crucially, our algorithm produced zero false positive fluid identifications across this study's 98 samples. Further optimization of the biomarker 'Codeset' will be required before it can be used in casework, particularly with respect to increasing the signal to noise ratio of the saliva biomarkers. With suitable modifications, this simplified protocol with minimal hands on requirement should facilitate routine use of mRNA profiling in casework laboratories.

scholarly journals MicroRNA markers for forensic body fluid identification obtained from miRCURY™ LNA array

2015 ◽  
Vol 5 ◽  
pp. e630-e632 ◽  
Author(s):  
X.Y. Luo ◽  
Z.L. Li ◽  
D. Peng ◽  
L. Wang ◽  
L. Zhang​ ◽  
...  
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification

mRNA profiling for body fluid identification

2008 ◽  
Vol 1 (1) ◽  
pp. 37-38 ◽  
Author(s):  
C. Haas ◽  
B. Klesser ◽  
A. Kratzer ◽  
W. Bär
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Profiling

Evaluation of the inclusion of circular RNAs in mRNA profiling in forensic body fluid identification

2017 ◽  
Vol 132 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Yaqi Zhang ◽  
Baonian Liu ◽  
Chengchen Shao ◽  
Hongmei Xu ◽  
Aimin Xue ◽  
...  
Keyword(s):  
Body Fluid ◽  
Circular Rnas ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Profiling

Optimal small-molecular reference RNA for RT-qPCR-based body fluid identification

2018 ◽  
Vol 37 ◽  
pp. 135-142 ◽  
Author(s):  
Shuntaro Fujimoto ◽  
Sho Manabe ◽  
Chie Morimoto ◽  
Munetaka Ozeki ◽  
Yuya Hamano ◽  
...  
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification
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