Genetic polymorphisms of 9 non-combined of DNA index system short tandem repeat loci of Chinese Tibetan ethnic minority group in Tibet

2011 ◽  
Vol 5 (4) ◽  
pp. 356-357 ◽  
Author(s):  
Xing-bo Song ◽  
MeiLangQuCuo ◽  
Di-jiao Tang ◽  
Ran Ren ◽  
Jia-ming Liu ◽  
...  
Keyword(s):  
Ethnic Minority ◽  
Genetic Polymorphisms ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Index System ◽  
Minority Group ◽  
Ethnic Minority Group ◽  
Dna Index ◽  
Short Tandem Repeat Loci ◽  
Short Tandem

scholarly journals Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil

2015 ◽  
Vol 14 (3) ◽  
pp. 7310-7314 ◽  
Author(s):  
R.G. Rodovalho ◽  
G.S. Santos ◽  
L.M. Cavalcanti ◽  
B.F.S.M. Moura ◽  
E.L. Rodrigues ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Index System ◽  
Allele Frequencies ◽  
Dna Index ◽  
Central Brazil ◽  
Short Tandem Repeat Loci ◽  
Short Tandem

Genetic polymorphisms of 20 short tandem repeat loci from the Han population in Henan, China

2014 ◽  
Vol 35 (10) ◽  
pp. 1509-1514 ◽  
Author(s):  
Hong-Dan Wang ◽  
Dong Wu ◽  
Zhan-Qi Feng ◽  
Zhi-An Jing ◽  
Tao Li ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Han Population ◽  
Short Tandem Repeat Loci ◽  
Short Tandem

scholarly journals The use of touch DNA analysis in forensic identification focusing on Short Tandem Repeat-Combined DNA Index System loci THO1, CSF1PO and TPOX

2020 ◽  
Author(s):  
Ahmad Yudianto ◽  
Indah Nuraini M. ◽  
Abdul Hadi Furqoni ◽  
Simon Martin Manyanza Nzilibili ◽  
Pudji Harjanto
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Index System ◽  
Dna Analysis ◽  
Forensic Identification ◽  
Buccal Swab ◽  
Dna Index ◽  
Touch Dna ◽  
Composite Gel ◽  
Short Tandem

Forensic identification through DNA analysis is an accurate diagnostic tool. Deoxyribonucleic Acid (DNA) analysis is via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short Tandem Repeat (STR). At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects, thereby transferring trace evidence to the objects. In this study DNA was obtained using “touch DNA” from two buccal smears and two smear from watches and cellphones from volunteers who had signed the consent form. Samples were isolated using DNAzol. The quantity of DNA obtained will be measured using a UV spectrophotometer. For DNA amplification using 3 STR CODIS loci namely TH01, CSF1PO, and TPOX. The last step is visualization using acrylamide gel and silver staining. Mean levels of DNA (UVVisible Spectrophotometer) were 167.89±85.71 μg/mL for the buccal swab, 59.19±5.58 μg/mL for the watch swab, and 38.09±2.12 μg/mL for the mobile swab; the purity of the buccal swab DNA was 1.79±0.71, of the watch swab 1.69±0.76, and of the mobile swab 1.53±0.56. Visualization of PCR products on Polyacrylamide Agarose Composite Gel Electrophoresis stained with Silver and amplified using the standard primers THOI, TPOX and CSF1PO for STR Combined DNA Index System (CODIS) showed a 100% detection of amplicons. Both the buccal swab, watch swab and handphone swabs had trace amount of DNA that was sufficient to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX.

Sign in / Sign up

Export Citation Format

Share Document