Cloning and expression of Malabar grouper ( Epinephelus malabaricus ) ADAR1 gene in response to immune stimulants and nervous necrosis virus

2017 ◽  
Vol 71 ◽  
pp. 116-126
Author(s):  
Thirunavukkarasu Periyasamy ◽  
Joan Tang Xiao Joe ◽  
Ming-Wei Lu
Keyword(s):  
Cloning And Expression ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Immune Stimulants ◽  
Epinephelus Malabaricus

scholarly journals Virus-Like Particles of a Fish Nodavirus Display a Capsid Subunit Domain Organization Different from That of Insect Nodaviruses

2002 ◽  
Vol 76 (12) ◽  
pp. 6370-6375 ◽  
Author(s):  
Liang Tang ◽  
Chan-Shing Lin ◽  
Neel K. Krishna ◽  
Mark Yeager ◽  
Anette Schneemann ◽  
...  
Keyword(s):  
Coat Protein ◽  
Three Dimensional ◽  
Nervous Necrosis Virus ◽  
Protein Fold ◽  
Norwalk Virus ◽  
Necrosis Virus ◽  
Virus Like Particles ◽  
Protein Fold Recognition ◽  
Dimensional Reconstruction ◽  
Epinephelus Malabaricus

ABSTRACT The structure of recombinant virus-like particles of malabaricus grouper nervous necrosis virus (MGNNV), a fish nodavirus isolated from the grouper Epinephelus malabaricus, was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-Å resolution. The cryoEM structure, sequence comparison, and protein fold recognition analysis indicate that the coat protein of MGNNV has two domains resembling those of tomato bushy stunt virus and Norwalk virus, rather than the expected single-domain coat protein of insect nodaviruses. The analysis implies that residues 83 to 216 fold as a β-sandwich which forms the inner shell of the T=3 capsid and residues 217 to 308 form the trimeric surface protrusions observed in the cryoEM map. The structural similarities between fish nodaviruses and members of the tombusvirus and calicivirus groups provide significant new data for understanding the evolution of the nodavirus family.

Characterization of red-spotted grouper nervous necrosis virus isolated from ovarian fluids of asymptomatic wild Asian seabass, Lates calcarifer

Aquaculture ◽  
2021 ◽  
pp. 736846
Author(s):  
Venkata Satyanarayana Nallala ◽  
M. Makesh ◽  
K. Radhika ◽  
T. Sathish Kumar ◽  
P. Raja ◽  
...  
Keyword(s):  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Lates Calcarifer ◽  
Asian Seabass

Grouper MAVS functions as a crucial antiviral molecule against nervous necrosis virus infection

2018 ◽  
Vol 72 ◽  
pp. 14-22 ◽  
Author(s):  
Youhua Huang ◽  
Jingcheng Zhang ◽  
Zhengliang Ouyang ◽  
Jiaxin Liu ◽  
Ya Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus

In vitro neutralization of nervous necrosis virus by a nanobody binding to the protrusion domain of capsid protein

Aquaculture ◽  
2021 ◽  
pp. 737654
Author(s):  
Song Zhu ◽  
Bo Miao ◽  
Yu-Zhou Zhang ◽  
Wei-Wei Zeng ◽  
De-Shou Wang ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus

scholarly journals Dietary Administration of Novel Multistrain Probiotics from Healthy Grouper Intestines Promotes the Intestinal Immune Response against NNV Infection

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1053
Author(s):  
Joan Tang Xiao Joe ◽  
Henry Tan Shi Sung ◽  
Jen-Leih Wu ◽  
Yu-Shen Lai ◽  
Ming-Wei Lu
Keyword(s):  
Challenge Test ◽  
Antiviral Effect ◽  
Viral Disease ◽  
Growth Conditions ◽  
Asia Pacific ◽  
Nervous Necrosis Virus ◽  
Potential Candidate ◽  
Necrosis Virus ◽  
And Cluster Analysis ◽  
Functional Pathway

Epinephelus lanceolatus (giant grouper) is a high-value cultured species in the Asia-Pacific region. However, nervous necrosis virus (NNV) is an infectious viral disease that affects over 120 species of marine cultured species and causes high mortality, ranging from 90–100% in the grouper industry. Probiotics isolated from the intestines of healthy individuals have provided insight into novel approaches involved in the defense against viral pathogens. In this study, we isolated three strains of bacteria as candidate probiotics from healthy grouper intestines and a 28-day feeding trial was performed. At day 21, the nervous necrosis virus (NNV) challenge test was conducted for 7 days to evaluate the antiviral effect of candidate probiotics. The results showed that candidate probiotics could improve growth conditions, such as weight gain (WG) and specific growth rate (SGR), and increase the utilization of feed. Furthermore, the candidate probiotic mixture had the ability to protect against NNV, which could decrease the mortality rate by 100% in giant grouper after NNV challenge. Subsequently, we analyzed the mechanism of the candidate probiotic mixture’s defense against NNV. A volcano plot revealed 203 (control vs. NNV), 126 (NNV vs. probiotics–NNV), and 5 (control vs. probiotics–NNV) differentially expressed transcripts in intestinal tissue. Moreover, principal components analysis (PCA) and cluster analysis heatmap showed large differences among the three groups. Functional pathway analysis showed that the candidate probiotic mixture could induce the innate and adaptive immunity of the host to defend against virus pathogens. Therefore, we hope that potential candidate probiotics could be successfully applied to the industry to achieve sustainable aquaculture.

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