Coagulation profile, gene expression and bioinformatics characterization of coagulation factor X of striped murrel Channa striatus

2016 ◽  
Vol 55 ◽  
pp. 149-158 ◽  
Author(s):  
Abirami Arasu ◽  
Venkatesh Kumaresan ◽  
Akila Sathyamoorthi ◽  
Mariadhas Valan Arasu ◽  
Naif Abdullah Al-Dhabi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coagulation Factor ◽  
Factor X ◽  
Channa Striatus ◽  
Coagulation Profile

Characterization of a homozygous Gly11Val mutation in the Gla domain of coagulation factor X

2009 ◽  
Vol 124 (1) ◽  
pp. 144-148 ◽  
Author(s):  
Ouerdia Chafa ◽  
Madjid Tagzirt ◽  
Jacqueline Tapon-Bretaudière ◽  
Abderrezak Reghis ◽  
Anne-Marie Fischer ◽  
...  
Keyword(s):  
Coagulation Factor ◽  
Factor X ◽  
Gla Domain

Characterization of the Murine Coagulation Factor X Promoter

2000 ◽  
Vol 84 (12) ◽  
pp. 1031-1038 ◽  
Author(s):  
Julie Wilberding ◽  
Francis Castellino
Keyword(s):  
Coagulation Factor ◽  
Proximal Promoter ◽  
Factor X ◽  
Wild Type ◽  
Gata Factor ◽  
Mobility Shift ◽  
Translational Initiation ◽  
Dnase I Footprinting ◽  
Shift Analysis

SummaryFactor X (FX) is a vitamin K-dependent serine protease zymogen that functions in both the extrinsic and intrinsic pathways of blood coagulation. In this study, the 5’-flanking region of the murine FX gene was analyzed to determine those elements that govern its transcriptional activity and regulation. Consistent with other TATA-less promoters, murine FX contains two start sites of transcription, at bp −5 and −21 relative to the ATG translational initiation codon. The mRNA of FX was found in a number of tissues, including the liver, stomach, intestine, kidney, ovary, testes, spleen, skeletal muscle, and lung. Using DNase I footprinting, three areas of protection have been identified in the proximal 287 bp of the promoter, spanning bp −28 to −218. Further examination of this region revealed transcription factor binding sites for NF-Y, HNF-4, and a GATA factor. Electrophoretic mobility shift analysis (EMSA) confirmed the identities of NF-Y, HNF-4, and GATA-4, all of which were found by transient transfection analyses in HepG2 cells to influence the activity of the promoter. Ablation of the NF-Y site was most dramatic, reducing activity to 10% of that of the wild-type construct. Deletion of the HNF-4 site led to an activity of 25% of wild-type, and a GATA-4 mutation reduced activity to 63% of wild-type values. This investigation revealed the identity of the factors bound at the proximal promoter of the FX gene, and the relative importance of each. This is the first report of a member of the GATA family of transcription factors being important in the regulation of a coagulation-based gene.

Congenital coagulation factor X deficiency: Genetic analysis of five patients and functional characterization of mutant factor X proteins

Haemophilia ◽  
2018 ◽  
Vol 24 (5) ◽  
pp. 774-785 ◽  
Author(s):  
Satomi Nagaya ◽  
Masashi Akiyama ◽  
Morika Murakami ◽  
Akiko Sekiya ◽  
Hidesaku Asakura ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Functional Characterization ◽  
Coagulation Factor ◽  
Factor X ◽  
Factor X Deficiency

Cloning and Characterization of a cDNA Encoding Murine Coagulation Factor X

1998 ◽  
Vol 80 (07) ◽  
pp. 87-91 ◽  
Author(s):  
Zhong Liang ◽  
Adrian Cooper ◽  
Melanie DeFord ◽  
Peter Carmeliet ◽  
Desire Collen ◽  
...  
Keyword(s):  
Stop Codon ◽  
Coagulation Factor ◽  
Factor X ◽  
Human Embryonic Kidney ◽  
Reading Frame ◽  
293 Cells ◽  
Murine Liver ◽  
Translation Initiation Codon ◽  
Amino Acid Signal

SummaryThe cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a λZap cDNA library generated from murine liver mRNA. The cDNA contains 1486 bases starting at the 5’-translation initiation codon. It includes an open reading frame of 1443 nucleotides, followed by an 18 residue 3’ nontranslated sequence downstream of the first stop codon, and a 3’ poly(A) tail. The translation product is composed of a 40-amino acid signal/propeptide region followed by a 441-residue mature protein. The latter is highly homologous to that of human and rat fX. All protein domains of human and rat fX are strictly conserved in mouse fX. The cDNA coding for mouse fX has been expressed in human embryonic kidney 293 cells and generates fX activity measured in a clotting assay using human fX-deficient plasma.

Isolation and characterization of human blood-coagulation factor X cDNA

Gene ◽  
1986 ◽  
Vol 41 (2-3) ◽  
pp. 311-314 ◽  
Author(s):  
Rajinder K. Kaul ◽  
Beth Hildebrand ◽  
Savithri Roberts ◽  
Pudur Jagadeeswaran
Keyword(s):  
Blood Coagulation ◽  
Human Blood ◽  
Coagulation Factor ◽  
Factor X ◽  
Isolation And Characterization ◽  
Human Blood Coagulation
Sign in / Sign up

Export Citation Format

Share Document