Development, validation and evaluation of a quantitative method for the analysis of twenty-four new psychoactive substances in oral fluid by LC–MS/MS

2020 ◽  
Vol 19 ◽  
pp. 100231 ◽  
Author(s):  
Travon Cooman ◽  
Heloa Santos ◽  
Joseph Cox ◽  
João Francisco Allochio Filho ◽  
Keyller Bastos Borges ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Oral Fluid ◽  
New Psychoactive Substances ◽  
Psychoactive Substances

Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC–MS-MS

2020 ◽  
Vol 44 (7) ◽  
pp. 697-707
Author(s):  
Kelly Francisco da Cunha ◽  
Karina Diniz Oliveira ◽  
Marilyn A Huestis ◽  
Jose Luiz Costa
Keyword(s):  
Oral Fluid ◽  
Drugs Of Abuse ◽  
Matrix Effects ◽  
Synthetic Cannabinoids ◽  
Screening Method ◽  
Simultaneous Detection ◽  
New Psychoactive Substances ◽  
Major Public Health Problem ◽  
Psychoactive Substances ◽  
Wide Range

Abstract New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography–tandem mass spectrometry (LC–MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid–elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, −55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7–127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and −20°C. As expected, greater stability was observed when samples were stored at −20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS.

Oral fluid drug analysis in the age of new psychoactive substances

Bioanalysis ◽  
2016 ◽  
Vol 8 (7) ◽  
pp. 691-710 ◽  
Author(s):  
Elisabeth Leere Øiestad ◽  
Åse Marit Leere Øiestad ◽  
Astrid Gjelstad ◽  
Ritva Karinen
Keyword(s):  
Oral Fluid ◽  
Drug Analysis ◽  
New Psychoactive Substances ◽  
Psychoactive Substances

Prevalence of new psychoactive substances (NPS) in Brazil based on oral fluid analysis of samples collected at electronic music festivals and parties

2021 ◽  
pp. 108962
Author(s):  
Kelly Francisco da Cunha ◽  
Karina Diniz Oliveira ◽  
Marilia Santoro Cardoso ◽  
Ana Carolina Furiozo Arantes ◽  
Pedro Henrique Piras Coser ◽  
...  
Keyword(s):  
Electronic Music ◽  
Oral Fluid ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Music Festivals ◽  
Fluid Analysis

Prevalence of New Psychoactive Substances in Oral Fluid Specimens from French and Belgian Drivers: Comparison 2016/2017

2018 ◽  
Vol 43 (2) ◽  
pp. e9-e10 ◽  
Author(s):  
Camille Richeval ◽  
Mélodie Nachon-Phanithavong ◽  
Vincent Di Fazio ◽  
Jean-François Wiart ◽  
Luc Humbert ◽  
...  
Keyword(s):  
Oral Fluid ◽  
New Psychoactive Substances ◽  
Psychoactive Substances

A Comprehensive HPLC–MS-MS Screening Method for 77 New Psychoactive Substances, 24 Classic Drugs and 18 Related Metabolites in Blood, Urine and Oral Fluid

2020 ◽  
Vol 44 (8) ◽  
pp. 769-783
Author(s):  
Annagiulia Di Trana ◽  
Giulio Mannocchi ◽  
Filippo Pirani ◽  
Nunzia La Maida ◽  
Massimo Gottardi ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Oral Fluid ◽  
Multiple Reaction Monitoring ◽  
Screening Method ◽  
Internal Standard ◽  
New Psychoactive Substances ◽  
Spectrometric Analysis ◽  
Blood Proteins ◽  
Psychoactive Substances ◽  
Better Than

Abstract To date, more than 800 molecules are classified as New Psychoactive Substances (NPS), and it is reported that this number increases every year. Whereas several cases of polydrug consumption that led to acute intoxication and death are reported, a lack of effective analytical screening method to detect NPS and classical drug of abuse in human matrices affects the prompt identification of the probable cause of intoxication in emergency department of hospitals. In this concern, a fast, simple and comprehensive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS-MS) screening method to detect and quantify 77 NPS, 24 classic drugs and 18 related metabolites has been successfully developed and validated in blood, urine and oral fluid. A small volume (100 µL) of whole blood samples spiked with internal standard deuterated mixture was added to 70 µL of M3® buffer, and after precipitation of blood proteins, the supernatant was evaporated to dryness and reconstituted in 1 mL of mobile phase. Same volume (100 µL) of urine and oral fluid samples spiked with internal standard deuterated mix were only diluted with 500 µL of M3® reagent. One microliter of samples of each matrix was injected into HPLC–MS-MS equipment. The run time lasted 10 min with a gradient mobile phase. Mass spectrometric analysis was performed in positive ion multiple reaction monitoring mode. The method was linear for all analytes under investigation with a determination coefficient always better than 0.99. The calibration range for blood and oral fluid was from limits of quantification (LOQs) to 200 ng/mL, whereas that for urine was LOQs to 1000 ng/mL. Recovery and matrix effect were always higher than 80%, whereas intra-assay and inter-assay precision were always better than 19% and accuracy was always within 19% of target in every matrix. Applicability of the method was verified by analysis of samples from real cases.

scholarly journals New psychoactive substances in oral fluid of drivers around a music festival in south-west France in 2017

2019 ◽  
Vol 297 ◽  
pp. 265-269 ◽  
Author(s):  
Camille Richeval ◽  
Véronique Dumestre-Toulet ◽  
Jean-François Wiart ◽  
Xavier Vanhoye ◽  
Luc Humbert ◽  
...  
Keyword(s):  
Oral Fluid ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Music Festival ◽  
South West

New psychoactive substances in oral fluid of French and Belgian drivers in 2016

2018 ◽  
Vol 57 ◽  
pp. 1-3 ◽  
Author(s):  
Camille Richeval ◽  
Sarah Maria Richarda Wille ◽  
Mélodie Nachon-Phanithavong ◽  
Nele Samyn ◽  
Delphine Allorge ◽  
...  
Keyword(s):  
Oral Fluid ◽  
New Psychoactive Substances ◽  
Psychoactive Substances
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