scholarly journals Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene

FEBS Open Bio ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Sedighe Karimi ◽  
Jana Wetzel ◽  
Johannes Wöstemeyer ◽  
Anke Burmester
Keyword(s):  
Auxotrophic Strain ◽  
Absidia Glauca

Regioselective O-glycosylation of flavonoids by fungi Beauveria bassiana, Absidia coerulea and Absidia glauca

2019 ◽  
Vol 93 ◽  
pp. 102750 ◽  
Author(s):  
Sandra Sordon ◽  
Jarosław Popłoński ◽  
Tomasz Tronina ◽  
Ewa Huszcza
Keyword(s):  
Beauveria Bassiana ◽  
Absidia Glauca ◽  
Absidia Coerulea

Genetic characterization and construction of an auxotrophic strain of Saccharomyces cerevisiae JP1, a Brazilian industrial yeast strain for bioethanol production

2012 ◽  
Vol 39 (11) ◽  
pp. 1673-1683 ◽  
Author(s):  
Viviane Castelo Branco Reis ◽  
André Moraes Nicola ◽  
Osmar de Souza Oliveira Neto ◽  
Vinícius Daniel Ferreira Batista ◽  
Lidia Maria Pepe de Moraes ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Strain ◽  
Genetic Characterization ◽  
Bioethanol Production ◽  
Industrial Yeast ◽  
Auxotrophic Strain ◽  
Industrial Yeast Strain

scholarly journals RelA Protein Is Involved in Induction of Genetic Competence in Certain Bacillus subtilis Strains by Moderating the Level of Intracellular GTP

2002 ◽  
Vol 184 (14) ◽  
pp. 3923-3930 ◽  
Author(s):  
Takashi Inaoka ◽  
Kozo Ochi
Keyword(s):  
Bacillus Subtilis ◽  
High Performance ◽  
Stationary Growth Phase ◽  
Competence Development ◽  
Stationary Growth ◽  
Transcriptional Fusion ◽  
Auxotrophic Strain ◽  
Genetic Competence ◽  
Physiological Conditions ◽  
Fusion Analysis

ABSTRACT We found that the ability to develop genetic competence of a certain relaxed (relA) aspartate-auxotrophic strain of Bacillus subtilis is significantly lower than that of the isogenic stringent (relA +) strain. Transcriptional fusion analysis utilizing a lacZ reporter gene indicated that the amount of the ComK protein, known as the key protein for competence development, is greatly reduced in the relaxed strain than in the stringent strain. We also found that the addition of decoyinine, a GMP synthetase inhibitor, induces expression of a competence gene (comG) in the relaxed strain, accompanied by a pronounced decrease in the level of intracellular GTP as measured by high-performance liquid chromatography. The transformation efficiency of the relaxed strain increased 100-fold when decoyinine was added at t0 (the transition point between exponential to stationary growth phase). Conversely, supplementation of guanosine together with decoyinine completely abolished the observed effect of adding decoyinine on competence development. Furthermore, the impaired ability of the relaxed strain for competence development was completely restored by disrupting the codY gene, which is known to negatively control comK expression. Our results indicate that the RelA protein plays an essential role in the induction of competence development at least under certain physiological conditions by reducing the level of intracellular GTP and overcoming CodY-mediated regulation.

Transformations of testosterone and related steroids in Absidia glauca culture

2003 ◽  
Vol 43 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Ewa Huszcza ◽  
Jadwiga Dmochowska-Gladysz
Keyword(s):  
Absidia Glauca

scholarly journals Saccharomyces cerevisiae Multidrug Resistance Transporter Qdr2 Is Implicated in Potassium Uptake, Providing a Physiological Advantage to Quinidine-Stressed Cells

2006 ◽  
Vol 6 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Rita C. Vargas ◽  
Raúl García-Salcedo ◽  
Sandra Tenreiro ◽  
Miguel C. Teixeira ◽  
Alexandra R. Fernandes ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Yeast Cell ◽  
Experimental Evidence ◽  
Growth Medium ◽  
Auxotrophic Strain ◽  
General Amino Acid Control ◽  
Stressed Cells ◽  
Multidrug Resistance Transporter

ABSTRACT The QDR2 gene of Saccharomyces cerevisiae encodes a putative plasma membrane drug:H+ antiporter that confers resistance against quinidine, barban, bleomycin, and cisplatin. This work provides experimental evidence of defective K+ (Rb+) uptake in the absence of QDR2. The direct involvement of Qdr2p in K+ uptake is reinforced by the fact that increased K+ (Rb+) uptake due to QDR2 expression is independent of the Trk1p/Trk2p system. QDR2 expression confers a physiological advantage for the yeast cell during the onset of K+ limited growth, due either to a limiting level of K+ in the growth medium or to the presence of quinidine. This drug decreases the K+ uptake rate and K+ accumulation in the yeast cell, especially in the Δqdr2 mutant. Qdr2p also helps to sustain the decrease of intracellular pH in quinidine-stressed cells in growth medium at pH 5.5 by indirectly promoting H+ extrusion affected by the drug. The results are consistent with the hypothesis that Qdr2p may also couple K+ movement with substrate export, presumably with quinidine. Other clues to the biological role of QDR2 in the yeast cell come from two additional lines of experimental evidence. First, QDR2 transcription is activated under nitrogen (NH4 +) limitation or when the auxotrophic strain examined enters stationary phase due to leucine limitation, this regulation being dependent on general amino acid control by Gcn4p. Second, the amino acid pool is higher in Δqdr2 cells than in wild-type cells, indicating that QDR2 expression is, directly or indirectly, involved in amino acid homeostasis.

scholarly journals Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca

FEBS Letters ◽  
1988 ◽  
Vol 234 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Hildegard Teepe ◽  
Jörg-Andreas Böttge ◽  
Johannes Wöstemeyer
Keyword(s):  
Electrophoretic Analysis ◽  
Surface Proteins ◽  
Absidia Glauca

Transfer of genetic information from the mycoparasite Parasitella parasitica to its host Absidia glauca

1993 ◽  
Vol 23 (4) ◽  
pp. 334-337 ◽  
Author(s):  
Martina Kellner ◽  
Anke Burmester ◽  
Anke W�stemeyer ◽  
Johannes W�stemeyer
Keyword(s):  
Genetic Information ◽  
Absidia Glauca
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