Campylobacter jejuni survival in a poultry processing plant environment

2017 ◽  
Vol 65 ◽  
pp. 185-192 ◽  
Author(s):  
Lourdes García-Sánchez ◽  
Beatriz Melero ◽  
Isabel Jaime ◽  
Marja-Liisa Hänninen ◽  
Mirko Rossi ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Processing Plant ◽  
Plant Environment ◽  
Poultry Processing

scholarly journals Comparison between conventional and qPCR methods for enumerating Campylobacter jejuni in a poultry processing plant

2011 ◽  
Vol 28 (7) ◽  
pp. 1353-1358 ◽  
Author(s):  
Beatriz Melero ◽  
Luca Cocolin ◽  
Kalliopi Rantsiou ◽  
Isabel Jaime ◽  
Jordi Rovira
Keyword(s):  
Campylobacter Jejuni ◽  
Processing Plant ◽  
Poultry Processing

Real-time PCR assay for rapid detection and quantification of Campylobacter jejuni on chicken rinses from poultry processing plant

2007 ◽  
Vol 21 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Aradom Debretsion ◽  
Tsegaye Habtemariam ◽  
Saul Wilson ◽  
David Nganwa ◽  
Teshome Yehualaeshet
Keyword(s):  
Real Time ◽  
Campylobacter Jejuni ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Processing Plant ◽  
Pcr Assay ◽  
Poultry Processing ◽  
Detection And Quantification

scholarly journals Prevalence of Campylobacter spp. in a poultry and pork processing plants

10.5219/1422 ◽  
2020 ◽  
Vol 14 ◽  
pp. 815-820
Author(s):  
Yuliya Yushina ◽  
Dagmara Bataeva ◽  
Anzhelika Makhova ◽  
Elena Zayko
Keyword(s):  
Campylobacter Jejuni ◽  
Central Region ◽  
Environmental Samples ◽  
Campylobacter Coli ◽  
Processing Plant ◽  
Processing Plants ◽  
Poultry Processing ◽  
Almost All ◽  
Campylobacter Spp

The study aimed to investigate the prevalence of Campylobacter spp. in different stages of poultry and pork processing in the Central region of Russia. A total of 47 Campylobacter isolates were obtained from 107 samples from poultry processing plants (40.2%): 87.2% were identified as Campylobacter jejuni, whereas 12.8% were identified as Campylobacter coli. The prevalence of Campylobacter was significantly (p <0.05) higher after evisceration in the poultry processing plant. Campylobacter spp.was detected in 62.7% of the equipment and environmental samples. From positive samples of Campylobacter spp., 84.3% of Campylobacter jejuni and 15.7% Campylobacter coli were observed. A total of nine Campylobacter isolates were obtained from 116 samples from pork processing plants (7.8%): 33.3% of them were identified as Campylobacter jejuni whereas 66.7% were identified as Campylobacter coli. Splitting and evisceration were also critical in Campylobacter contamination. Almost all pork carcasses were Campylobacter positive, and all of them were identified as Campylobacter coli. The prevalence of positive Campylobacter samples in poultry processing plants was significantly (p < 0.05) higher than in pork processing plants.

Electrical Treatment of Poultry Chiller Water to Destroy Campylobacter jejuni

1995 ◽  
Vol 58 (12) ◽  
pp. 1330-1334 ◽  
Author(s):  
YANBIN LI ◽  
JOEL T. WALKER ◽  
MICHAEL F. SLAVIK ◽  
HONG WANG
Keyword(s):  
Sodium Chloride ◽  
Campylobacter Jejuni ◽  
Salt Concentration ◽  
Salt Solution ◽  
Death Rate ◽  
Bacterial Contamination ◽  
Treatment Time ◽  
Processing Plant ◽  
Colony Forming Units ◽  
Poultry Processing

To control bacterial contamination in poultry processing, pulsed electricity in combination with a salt was evaluated as an electrical pasteurization method to kill Campylobacter jejuni in poultry chiller water. Chiller water from a poultry processing plant was mixed with either sodium chloride (NaCl) or trisodium phosphate (Na3PO4 12H2O or TSP) at 0.1 %, 0.2% or 0.3% concentration, and inoculated with C. jejuni at 1 × 106 CFU/ml. The inoculated chiller water was treated at 4°C for 20 min using pulsed electrical signals at 10mA/cm2 current, 1 kHz frequency and 50% duty cycle. Samples taken at different intervals were serially diluted, pre-enriched in Brucella-FBP broth, plated on Campy-Cefex agar and incubated, and colony-forming units (CFU) were counted. The results showed that C. jejuni was reduced and that the bacterial death rate was dependent upon pH of the salt solution, salt concentration, and treatment time. In the electrical treatments, C. jejuni was reduced linearly on log scale in the chiller water mixed with TSP, but nonlinearly with NaCl. Bacterial destruction rate was accelerated by higher concentrations or higher pH of NaCl or TSP added to chiller water.

scholarly journals Genetic Listeria monocytogenes Types in the Pork Processing Plant Environment: From Occasional Introduction to Plausible Persistence in Harborage Sites

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 717
Author(s):  
Niels Demaître ◽  
Geertrui Rasschaert ◽  
Lieven De Zutter ◽  
Annemie Geeraerd ◽  
Koen De Reu
Keyword(s):  
Genetic Diversity ◽  
Environmental Samples ◽  
Genetic Characterization ◽  
Processing Plant ◽  
Pfge Analysis ◽  
Food Contact ◽  
Plant Environment ◽  
Food Contact Surfaces ◽  
Contact Surfaces ◽  
Cleaning And Disinfection

The purpose of this study was to investigate the L. monocytogenes occurrence and genetic diversity in three Belgian pork cutting plants. We specifically aim to identify harborage sites and niche locations where this pathogen might occur. A total of 868 samples were taken from a large diversity of food and non-food contact surfaces after cleaning and disinfection (C&D) and during processing. A total of 13% (110/868) of environmental samples tested positive for L. monocytogenes. When looking in more detail, zone 3 non-food contact surfaces were contaminated more often (26%; 72/278) at typical harborage sites, such as floors, drains, and cleaning materials. Food contact surfaces (zone 1) were less frequently contaminated (6%; 25/436), also after C&D. PFGE analysis exhibited low genetic heterogeneity, revealing 11 assigned clonal complexes (CC), four of which (CC8, CC9, CC31, and CC121) were predominant and widespread. Our data suggest (i) the occasional introduction and repeated contamination and/or (ii) the establishment of some persistent meat-adapted clones in all cutting plants. Further, we highlight the importance of well-designed extensive sampling programs combined with genetic characterization to help these facilities take corrective actions to prevent transfer of this pathogen from the environment to the meat.

Diversity of flaA Genotypes among Campylobacter jejuni Isolated from Six Niche-Market Poultry Species at Farm and Processing

2006 ◽  
Vol 69 (2) ◽  
pp. 299-307 ◽  
Author(s):  
C. VanWORTH ◽  
B. A. McCREA ◽  
K. H. TONOOKA ◽  
C. L. BOGGS ◽  
J. S. SCHRADER
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Campylobacter Jejuni ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Guinea Fowl ◽  
Processing Plant ◽  
Cross Contamination ◽  
Free Range ◽  
Processing Plants ◽  
Restriction Fragment Length

PCR–restriction fragment length polymorphism of the flagellin (flaA) gene in Campylobacter jejuni was used to determine the relationships of isolates collected at the farm and throughout processing for six niche-market poultry species. This study focused on two specialty chicken products, poussin and free range, and four other specialty products, squab, duck, guinea fowl, and quail. Cloacal and carcass samples were collected from three flocks from each of the six niche species. Three processing plants in California participated in a 2-year investigation. A total of 773 isolates from farm, posttransport, and the processing plants were genotyped, yielding a total of 72 distinct flaA profiles for the six commodities. Genetic diversity of C. jejuni at the farm was greatest for ducks with up to 12 distinct flaA types in two flocks and least for squab 1 flaA type between two farms. For two of the guinea fowl flocks, one free-range flock, two squab flocks, and all three poussin flocks, the flaA types recovered at the prepackage station matched those from the farm. Cross-contamination of poultry carcasses was supported by the observation of flaA types during processing that were not present at the farm level. New C. jejuni strains were detected after transport in ducks, guinea fowl, and free-range chickens. Postpicker, postevisceration, and prewash sampling points in the processing plant yield novel isolates. Duck and free-range chickens were the only species for which strains recovered within the processing plant were also found on the final product. Isolates recovered from squab had 56 to 93% similarity based on the flaA types defined by PCR–restriction fragment length polymorphism profiles. The 26 duck isolates had genetic similarities that ranged from 20 to 90%. Guinea fowl and free-range chickens each had 40 to 65% similarity between isolates. Poussin isolates were 33 to 55% similar to each other, and quail isolates were 46 to 100% similar. Our results continue to emphasize the need to clean processing equipment and posttransport crates in order to decrease cross contamination between flocks. This study also determined that several strains of C. jejuni had unique flaA types that could only be recovered in their host species.

Microbiological survey of a South African poultry processing plant

1995 ◽  
Vol 35 (2) ◽  
pp. 73-82 ◽  
Author(s):  
Ifigenia Geornaras ◽  
Amelia De Jesus ◽  
Elsabé Van Zyl ◽  
Alexander Von Holy
Keyword(s):  
South African ◽  
Processing Plant ◽  
Poultry Processing

scholarly journals Holistic Approaches to Reducing Salmonella Contamination in Poultry Industry

2021 ◽  
Vol 44 (1) ◽  
Author(s):  
Ummu Afiqah Abdul-Rahiman ◽  
Noordiana Nordin ◽  
Noor Azira Abdul-Mutalib ◽  
Maimunah Sanny
Keyword(s):  
Hazard Analysis ◽  
Monitoring Program ◽  
Published Data ◽  
Processing Plant ◽  
Poultry Production ◽  
Poultry Industry ◽  
Production Chain ◽  
Critical Control Points ◽  
Poultry Processing ◽  
And Control

Salmonella are widely found in the poultry industry, which subsequently may pose a risk to animal and human health. The aim of this review is to highlight strategies for the prevention and control of Salmonella at each stage in the poultry production chain by monitoring risks from the farm to the retailer. Among the primary approaches for control of Salmonella at the farm level includes the administration of synthetic and natural compounds to live chickens (vaccination and antibiotic), litter management as well as fortification of feed and acidification of drinking water. In the poultry processing plant, multiple hurdle technology and different chilling conditions to reduce Salmonella were discussed. In the retail level, an effective monitoring program to control Salmonella contamination by good manufacturing practices and hazard analysis and critical control points has been reviewed. Overall, we conclude that these approaches play a role in reducing the dissemination of Salmonella in the poultry industry. However, there is no published data related to logistic scheduling of poultry processing.

Traceback Identification of an Ingredient (Pork Dewlap) as the Possible Source of Listeria monocytogenes Serotype 4b Contamination in Raw Chicken Products

2007 ◽  
Vol 70 (6) ◽  
pp. 1513-1517 ◽  
Author(s):  
VICTORIA LÓPEZ ◽  
SAGRARIO ORTIZ ◽  
ALFREDO CORUJO ◽  
PILAR LÓPEZ ◽  
JAIME NAVAS ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Restriction Analysis ◽  
Virulence Gene ◽  
Pulsed Field Gel Electrophoresis ◽  
Processing Plant ◽  
Poultry Processing ◽  
Serotype 4B ◽  
Fresh Products ◽  
Allelic Analysis

In surveys conducted on finished product samples from a single poultry processing plant in Spain, Listeria monocytogenes was found in 14 different uncooked products. To track contamination patterns, 77 L. monocytogenes isolates were characterized by PCR-based serotyping, pulsed-field gel electrophoresis (PFGE) restriction analysis, and PCR-based allelic analysis of the virulence gene actA. Serotyping revealed that 12 isolates (15.6%) were of the L. monocytogenes serotype 4b complex (serotype 4b or the closely related serotypes 4d and 4e). A combination of endonucleases AscI and ApaI PFGE patterns yielded 15 different pulsotypes among all 77 tested isolates. All the serotype 4b isolates belonged to one pulsotype. Sequencing of the actA gene confirmed that all serotype 4b isolates corresponded to the same allelic subtype. The subtype was recovered from five product types, but its presence was not correlated with the production line or the date of isolation, suggesting a possible association of this strain with a common ingredient. This traceback investigation established that pork dewlap, an ingredient common to all the products contaminated with this strain, was the most probable source of L. monocytogenes 4b. The same 4b strain was isolated from four samples of pork dewlap from one specific supplier. After replacement of this contaminated ingredient in the fresh products, this strain of L. monocytogenes serotype 4b was not detected. This study confirms the effectiveness of molecular subtyping to control contamination by specific strains of L. monocytogenes and the importance of testing the different ingredients added to the food products.

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