Inactivation of feline calicivirus and murine norovirus during Dongchimi fermentation

2012 ◽  
Vol 31 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Min Hwa Lee ◽  
Seung-Hee Yoo ◽  
Sang-Do Ha ◽  
Changsun Choi
Keyword(s):  
Feline Calicivirus ◽  
Murine Norovirus

scholarly journals Evaluation of Murine Norovirus, Feline Calicivirus, Poliovirus, and MS2 as Surrogates for Human Norovirus in a Model of Viral Persistence in Surface Water and Groundwater

2007 ◽  
Vol 74 (2) ◽  
pp. 477-484 ◽  
Author(s):  
Jinhee Bae ◽  
Kellogg J. Schwab
Keyword(s):  
Cell Culture ◽  
Surface Water ◽  
Multiple Linear Regression Model ◽  
Viral Persistence ◽  
Environmental Stability ◽  
Great Promise ◽  
Feline Calicivirus ◽  
Virus Infectivity ◽  
Murine Norovirus ◽  
Surface Water And Groundwater

ABSTRACT Human noroviruses (NoVs) are a significant cause of nonbacterial gastroenteritis worldwide, with contaminated drinking water a potential transmission route. The absence of a cell culture infectivity model for NoV necessitates the use of molecular methods and/or viral surrogate models amenable to cell culture to predict NoV inactivation. The NoV surrogates murine NoV (MNV), feline calicivirus (FCV), poliovirus (PV), and male-specific coliphage MS2, in conjunction with Norwalk virus (NV), were spiked into surface water samples (n = 9) and groundwater samples (n = 6). Viral persistence was monitored at 25°C and 4°C by periodically analyzing virus infectivity (for all surrogate viruses) and nucleic acid (NA) for all tested viruses. FCV infectivity reduction rates were significantly higher than those of the other surrogate viruses. Infectivity reduction rates were significantly higher than NA reduction rates at 25°C (0.18 and 0.09 log10/day for FCV, 0.13 and 0.10 log10/day for PV, 0.12 and 0.06 log10/day for MS2, and 0.09 and 0.05 log10/day for MNV) but not significant at 4°C. According to a multiple linear regression model, the NV NA reduction rates (0.04 ± 0.01 log10/day) were not significantly different from the NA reduction rates of MS2 (0.05 ± 0.03 log10/day) and MNV (0.04 ± 0.03 log10/day) and were significantly different from those of FCV (0.08 ± 0.03 log10/day) and PV (0.09 ± 0.03 log10/day) at 25°C. In conclusion, MNV shows great promise as a human NoV surrogate due to its genetic similarity and environmental stability. FCV was much less stable and thus questionable as an adequate surrogate for human NoVs in surface water and groundwater.

Comparison of the antiviral activity of flavonoids against murine norovirus and feline calicivirus

Food Control ◽  
2016 ◽  
Vol 60 ◽  
pp. 25-30 ◽  
Author(s):  
Dong Joo Seo ◽  
Su Been Jeon ◽  
Hyejin Oh ◽  
Bog-Hieu Lee ◽  
Sook-Young Lee ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Feline Calicivirus ◽  
Murine Norovirus

scholarly journals Survivin Overexpression Has a Negative Effect on Feline Calicivirus Infection

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 996
Author(s):  
Oscar Salvador Barrera-Vázquez ◽  
Clotilde Cancio-Lonches ◽  
Carlos Emilio Miguel-Rodríguez ◽  
Monica Margarita Valdes Pérez ◽  
Ana Lorena Gutiérrez-Escolano
Keyword(s):  
Virus Production ◽  
Feline Calicivirus ◽  
Protective Effects ◽  
Murine Norovirus ◽  
Progeny Production ◽  
Apoptotic Protein ◽  
Viral Yield ◽  
Viral Progeny ◽  
Negative Effect ◽  
Replicative Cycle

It is known that levels of the anti-apoptotic protein survivin are reduced during Murine norovirus MNV-1 and Feline calicivirus (FCV) infection as part of the apoptosis establishment required for virus release and propagation in the host. Recently, our group has reported that overexpression of survivin causes a reduction of FCV protein synthesis and viral progeny production, suggesting that survivin may affect early steps of the replicative cycle. Using immunofluorescence assays, we observed that overexpression of survivin, resulted in the reduction of FCV infection not only in transfected but also in the neighboring nontransfected CrFK cells, thus suggesting autocrine and paracrine protective effects. Cells treated with the supernatants collected from CrFK cells overexpressing survivin showed a reduction in FCV but not MNV-1 protein production and viral yield, suggesting that FCV binding and/or entry were specifically altered. The reduced ability of FCV to bind to the surface of the cells overexpressing survivin, or treated with the supernatants collected from these cells, correlate with the reduction in the cell surface of the FCV receptor, the feline junctional adhesion molecule (fJAM) 1, while no effect was observed in the cells transfected with the pAm-Cyan vector or in cells treated with the corresponding supernatants. Moreover, the overexpression of survivin affects neither Vaccinia virus (VACV) production in CrFK cells nor MNV-1 virus production in RAW 267.4 cells, indicating that the effect is specific for FCV. All of these results taken together indicate that cells that overexpress survivin, or cell treatment with the conditioned medium from these cells, results in the reduction of the fJAM-1 molecule and, therefore, a specific reduction in FCV entry and infection.

scholarly journals Antiviral Effect of Korean Red Ginseng Extract and Ginsenosides on Murine Norovirus and Feline Calicivirus as Surrogates for Human Norovirus

2011 ◽  
Vol 35 (4) ◽  
pp. 429-435 ◽  
Author(s):  
Min-Hwa Lee ◽  
Bog-Hieu Lee ◽  
Ji-Youn Jung ◽  
Doo-Sung Cheon ◽  
Kyung-Tack Kim ◽  
...  
Keyword(s):  
Antiviral Effect ◽  
Feline Calicivirus ◽  
Murine Norovirus ◽  
Red Ginseng ◽  
Human Norovirus ◽  
Korean Red Ginseng ◽  
Ginseng Extract

scholarly journals Feline calicivirus- and murine norovirus-induced COX-2/PGE2 signaling pathway has proviral effects

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0200726 ◽  
Author(s):  
Mia Madel Alfajaro ◽  
Eun-Hyo Cho ◽  
Jun-Gyu Park ◽  
Ji-Yun Kim ◽  
Mahmoud Soliman ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Feline Calicivirus ◽  
Murine Norovirus ◽  
Cox 2

Comparative Efficacy of Seven Hand Sanitizers against Murine Norovirus, Feline Calicivirus, and GII.4 Norovirus†

2010 ◽  
Vol 73 (12) ◽  
pp. 2232-2238 ◽  
Author(s):  
GEUN WOO PARK ◽  
LESLIE BARCLAY ◽  
DAVID MACINGA ◽  
DUANE CHARBONNEAU ◽  
CHARLES A. PETTIGREW ◽  
...  
Keyword(s):  
Plaque Assay ◽  
Feline Calicivirus ◽  
Murine Norovirus ◽  
Comparative Efficacy ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Source Of Infection ◽  
Fecal Extract ◽  
Virucidal Efficacy

Contaminated hands or inanimate surfaces can act as a source of infection during outbreaks of human norovirus infection. We evaluated the virucidal efficacy of seven hand sanitizers containing various active ingredients, such as ethanol, triclosan, and chlorhexidine, and compared their effectiveness against feline calicivirus (FCV), murine norovirus (MNV), and a GII.4 norovirus fecal extract. We also tested the efficacy of 50, 70, and 90% of ethanol and isopropanol. Reduction of viral infectivity was measured by plaque assay, and the number of genomic copies was determined with a TaqMan real-time reverse transcription PCR assay. Based on the results of a quantitative suspension test, only one ethanol-based product (72% ethanol, pH 2.9) and one triclosan-based product (0.1% triclosan, pH 3.0) reduced the infectivity of both MNV and FCV (by >2.6 and ≥3.4 log units, respectively). Four of the seven products were effective against either MNV or FCV, whereas chlorhexidine was ineffective against both viruses. For these hand sanitizers, no correlation was found between reduced infectivity and decline of viral RNA. Ethanol and isopropanol concentrations ≥70% reduced the infectivity of MNV by ≥2.6 log units, whereas 50 and 70% ethanol reduced the infectivity of FCV by ≥2.2 log units after exposure for 5 min. The susceptibility of FCV to low pH and the relative high susceptibility of MNV to alcohols suggest that both surrogate viruses should be considered for in vitro testing of hand sanitizers.

scholarly journals Structures of the Compact Helical Core Domains of Feline Calicivirus and Murine Norovirus VPg Proteins

2013 ◽  
Vol 87 (10) ◽  
pp. 5318-5330 ◽  
Author(s):  
E. N. Leen ◽  
K. Y. R. Kwok ◽  
J. R. Birtley ◽  
P. J. Simpson ◽  
C. V. Subba-Reddy ◽  
...  
Keyword(s):  
Feline Calicivirus ◽  
Murine Norovirus

Surrogates for the Study of Norovirus Stability and Inactivation in the Environment: A Comparison of Murine Norovirus and Feline Calicivirus

2006 ◽  
Vol 69 (11) ◽  
pp. 2761-2765 ◽  
Author(s):  
JENNIFER L. CANNON ◽  
EFSTATHIA PAPAFRAGKOU ◽  
GEUNWOO W. PARK ◽  
JASON OSBORNE ◽  
LEE-ANN JAYKUS ◽  
...  
Keyword(s):  
Cell Culture ◽  
Thermal Inactivation ◽  
Genetic Relatedness ◽  
Mammalian Cell Culture ◽  
Feline Calicivirus ◽  
Murine Norovirus ◽  
Log Reduction ◽  
Culture Model ◽  
Dry Conditions ◽  
Ph Range

Human noroviruses (NoVs) are the leading cause of food- and waterborne outbreaks of acute nonbacterial gastroenteritis worldwide. As a result of the lack of a mammalian cell culture model for these viruses, studies on persistence, inactivation, and transmission have been limited to cultivable viruses, including feline calicivirus (FCV). Recently, reports of the successful cell culture of murine norovirus 1 (MNV-1) have provided investigators with an alternative surrogate for human NoVs. In this study, we compared the inactivation profiles of MNV-1 to FCV in an effort to establish the relevance of MNV-1 as a surrogate virus. Specifically, we evaluated (i) stability upon exposure to pH extremes; (ii) stability upon exposure to organic solvents; (iii) thermal inactivation; and (iv) surface persistence under wet and dry conditions. MNV-1 was stable across the entire pH range tested (pH 2 to 10) with less than 1 log reduction in infectivity at pH 2, whereas FCV was inactivated rapidly at pH values <3 and >9. FCV was more stable than MNV-1 at 56°C, but both viruses exhibited similar inactivation at 63 and 72°C. Long-term persistence of both viruses suspended in a fecal matrix and inoculated onto stainless steel coupons were similar at 4°C, but at room temperature in solution, MNV-1 was more stable than FCV. The genetic relatedness of MNV-1 to human NoVs combined with its ability to survive under gastric pH levels makes this virus a promising and relevant surrogate for studying environmental survival of human NoVs.

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