Isolation, enumeration and PCR characterization of aflatoxigenic fungi from food and feed samples in India

2004 ◽  
Vol 21 (6) ◽  
pp. 809-813 ◽  
Author(s):  
D Somashekar ◽  
E.R Rati ◽  
S Anand ◽  
A Chandrashekar
Keyword(s):  
Aflatoxigenic Fungi ◽  
Food And Feed ◽  
Feed Samples

scholarly journals Characterization of aflatoxin producing Aspergillus flavus from food and feed samples

SpringerPlus ◽  
2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Md Fakruddin ◽  
Abhijit Chowdhury ◽  
Md Nur Hossain ◽  
Monzur Morshed Ahmed
Keyword(s):  
Aspergillus Flavus ◽  
Food And Feed ◽  
Feed Samples

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

A multicomponent method for Fusarium toxins in cereal based food and feed samples using HPLC-MS/MS

2005 ◽  
Vol 21 (1) ◽  
pp. 18-22 ◽  
Author(s):  
S. Biselli ◽  
H. Wegner ◽  
C. Hummert
Keyword(s):  
Fusarium Toxins ◽  
Food And Feed ◽  
Feed Samples

scholarly journals Calculation of Measurement Uncertainty in Quantitative Analysis of Genetically Modified Organisms Using Intermediate PrecisionA Practical Approach

2007 ◽  
Vol 90 (2) ◽  
pp. 582-586 ◽  
Author(s):  
Jana Žel ◽  
Kristina Gruden ◽  
Katarina Cankar ◽  
Dejan tebih ◽  
Andrej Blejec
Keyword(s):  
Nucleic Acids ◽  
Measurement Uncertainty ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Laboratory Diagnostics ◽  
Quantitative Characterization ◽  
Intermediate Precision ◽  
Food And Feed ◽  
Repeatability And Reproducibility

Abstract Quantitative characterization of nucleic acids is becoming a frequently used method in routine analysis of biological samples, one use being the detection of genetically modified organisms (GMOs). Measurement uncertainty is an important factor to be considered in these analyses, especially where precise thresholds are set in regulations. Intermediate precision, defined as a measure between repeatability and reproducibility, is a parameter describing the real situation in laboratories dealing with quantitative aspects of molecular biology methods. In this paper, we describe the top-down approach to calculating measurement uncertainty, using intermediate precision, in routine GMO testing of food and feed samples. We illustrate its practicability in defining compliance of results with regulations. The method described is also applicable to other molecular methods for a variety of laboratory diagnostics where quantitative characterization of nucleic acids is needed.

Characterization of protein, lipid and mineral contents in common Norwegian seaweeds and evaluation of their potential as food and feed

2014 ◽  
Vol 94 (15) ◽  
pp. 3281-3290 ◽  
Author(s):  
Hanne K Maehre ◽  
Marian K Malde ◽  
Karl-Erik Eilertsen ◽  
Edel O Elvevoll
Keyword(s):  
Mineral Contents ◽  
Food And Feed

Granulometric Characterization of Flotation Products

2012 ◽  
Vol 727-728 ◽  
pp. 1902-1907
Author(s):  
R.C. Santana ◽  
M.A. Santos ◽  
T.C.M. Santos ◽  
T.A.A. Souza ◽  
Carlos Henrique Ataíde ◽  
...  
Keyword(s):  
Particle Size ◽  
Fine Particle ◽  
Column Height ◽  
Particle Size Fractions ◽  
Granulometric Analysis ◽  
Fine Particle Size ◽  
Distribution Parameters ◽  
Parameter Values ◽  
Feed Samples

This work aimed on the granulometric characterization of flotation products and to analyze the effect of the variables (that were investigated in a flotation work) on the particle size distribution parameters. Besides to compare the parameter values of the granulometric analysis for the concentrate and tailing both results were compared with the parameter values of the flotation feed samples. The analyzed parameters showed lower values for the concentrate products as compared with the parameters for tailing and feed samples. For coarse and fine particle size fractions the column height was the variable that more influenced on the parameter values.

scholarly journals Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS

2017 ◽  
Vol 239 ◽  
pp. 1087-1097 ◽  
Author(s):  
Katrina Campbell ◽  
Ana L. Ferreira Cavalcante ◽  
Pamela Galvin-King ◽  
Michalina Oplatowska-Stachowiak ◽  
Catherine Brabet ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Food And Feed ◽  
Feed Samples
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