Phase equilibrium and protein partitioning in aqueous micellar two-phase system composed of surfactant and polymer

2012 ◽  
Vol 320 ◽  
pp. 60-64 ◽  
Author(s):  
Yang Liu ◽  
Zhongyang Wu ◽  
Jinhong Dai
Keyword(s):  
Phase Equilibrium ◽  
Phase System ◽  
Two Phase ◽  
Protein Partitioning

Formulation of a General Multiphase, Multicomponent Chemical Flood Model

1981 ◽  
Vol 21 (01) ◽  
pp. 63-76 ◽  
Author(s):  
Paul D. Fleming ◽  
Charles P. Thomas ◽  
William K. Winter
Keyword(s):  
Phase Equilibrium ◽  
Arbitrary Number ◽  
Numerical Solutions ◽  
Field Tests ◽  
Reservoir Rock ◽  
Phase System ◽  
Surfactant Flooding ◽  
Two Phase ◽  
Special Cases ◽  
Flood Model

Abstract A general multiphase, multicomponent chemical flood model has been formulated. The set of mass conservation laws for each component in an isothermal system is closed by assuming local thermodynamic (phase) equilibrium, Darcy's law for multiphase flow through porous media, and Fick's law of diffusion. For the special case of binary, two-phase flow of nonmixing incompressible fluids, the equations reduce to those of Buckley and Leverett. The Buckley-Leverett equations also may be obtained for significant fractions of both components in the phases if the two phases are sufficiently incompressible. To illustrate the usefulness of the approach, a simple chemical flood model for a ternary, two-phase system is obtained which can be applied to surfactant flooding, polymer flooding, caustic flooding, etc. Introduction Field tests of various forms of surfactant flooding currently are under way or planned at a number of locations throughout the country.1 The chemical systems used have become quite complicated, often containing up to six components (water, oil, surfactant, alcohol, salt, and polymer). The interactions of these components with each other and with the reservoir rock and fluids are complex and have been the subject of many laboratory investigations.2–22 To aid in organizing and understanding laboratory work, as well as providing a means of extrapolating laboratory results to field situations, a mathematical description of the process is needed. Although it seems certain that mathematical simulations of such processes are being performed, models aimed specifically at the process have been reported only recently in the literature.23–31 It is likely that many such simulations are being performed on variants of immiscible, miscible, and compositional models that do not account for all the facets of a micellar/polymer process. To help put the many factors of such a process in proper perspective, a generalized model has been formulated incorporating an arbitrary number of components and an arbitrary number of phases. The development assumes isothermal conditions and local phase equilibrium. Darcy's law32,33 is assumed to apply to the flow of separate phases, and Fick's law34 of diffusion is applied to components within a phase. The general development also provides for mass transfer of all components between phases, the adsorption of components by the porous medium, compressibility, gravity segregation effects, and pressure differences between phases. With the proper simplifying assumptions, the general model is shown to degenerate into more familiar special cases. Numerical solutions of special cases of interest are presented elsewhere.35

Liquid–liquid phase equilibrium of MgSO4and PEG1500 aqueous two-phase system

2010 ◽  
Vol 48 (6) ◽  
pp. 764-772 ◽  
Author(s):  
R. Azimaie ◽  
G.R. Pazuki ◽  
V. Taghikhani ◽  
M. Vossoughi ◽  
Cyrus Ghotbi
Keyword(s):  
Phase Equilibrium ◽  
Liquid Phase ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Optimisation of LS54/Dx Aqueous Two Phase System Conditions for Cutinase Recovery

2013 ◽  
Vol 12 (2) ◽  
pp. 61
Author(s):  
Jamaliah Md. Jahim ◽  
Abdul Wahab Mohamad ◽  
Fariza Akmal Abdul Mutalib ◽  
Farah Diba Abu Bakar ◽  
Osman Hassan
Keyword(s):  
Recovery Process ◽  
Line Length ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase System ◽  
System Composition ◽  
Protein Partitioning ◽  
Tie Line ◽  
Positive Electrostatic Potential

Aqueous two phase system comprising Dehypon® LS 54 and K4484 Dextrin® was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30% of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore, the addition of 50mM salts such as K2SO4 and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcut to 2.2 and 1.95 fold, respectively. The dependence of kcut on various additives such as (NH4)2SO4, Na2SO4 and K2SO4 at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23% and Na2SO4 = 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79% can be achieved.

Phase Equilibrium of the Cs2SO4-C2H5OH-H2O Aqueous Two-Phase System

2003 ◽  
Vol 19 (11) ◽  
pp. 1089-1092
Author(s):  
Zhai Quan-Guo ◽  
◽  
Hu Man-Cheng ◽  
Liu Zhi-Hong ◽  
Xia Shu-Ping ◽  
...  
Keyword(s):  
Phase Equilibrium ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Application of the response surface methodology for optimization of whey protein partitioning in PEG/phosphate aqueous two-phase system

2011 ◽  
Vol 879 (21) ◽  
pp. 1881-1885 ◽  
Author(s):  
Lizzy Ayra Pereira Alcântara ◽  
Luis Antonio Minim ◽  
Valéria Paula Rodrigues Minim ◽  
Renata Cristina Ferreira Bonomo ◽  
Luis Henrique Mendes da Silva ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Whey Protein ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Protein Partitioning
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