Plenary reviews of the 28th Fungal Genetics Conference

2016 ◽  
Vol 90 ◽  
pp. 23
Author(s):  
Nancy Keller
Keyword(s):  
Fungal Genetics

Aspects of Fungal Genetics

1987 ◽  
pp. 109-133
Author(s):  
Brian W. Bainbridge
Keyword(s):  
Fungal Genetics

Fungal Genetics

Fungi ◽  
2017 ◽  
pp. 91-118
Author(s):  
Brendan Curran ◽  
Virginia Bugeja
Keyword(s):  
Fungal Genetics

Fungal genetics

2017 ◽  
Author(s):  
Paul S. Dyer ◽  
Carol A Munro ◽  
Rosie E. Bradshaw
Keyword(s):  
Genetic Analysis ◽  
Cellular Localization ◽  
Gene Knockout ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Model Organisms ◽  
Medical Mycology ◽  
Fungal Genetics ◽  
Study Gene Expression ◽  
Fluorescent Tagging

Fungi have been long used as model organisms to investigate genetic and cellular processes. An overview is provided of how fungi function at a genetic level, including ploidy, gene structure, and gene flow by sexual and asexual processes. The tools used to study fungal genetics are then described, such techniques having widespread applications in medical mycology research. Classical genetic analysis includes the use of gene mapping by sexual crossing and tetrad analysis, and forward genetic experimentation based on mutagenesis, for which various mutant screening approaches are described. Molecular genetic analysis includes gene manipulation by transformation; different methods for gene knockout and targeting, and their application for forward and reverse genetic approaches, are outlined. Finally, molecular genetic methods used to study gene expression and function are reviewed, including use of inducible or constitutive overexpression, real-time PCR, cellular localization of gene products by fluorescent tagging, and detection of protein–protein interactions.

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