scholarly journals Functional characterization of a novel Mn2+ dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578

FEBS Letters ◽  
2012 ◽  
Vol 586 (21) ◽  
pp. 3778-3786 ◽  
Author(s):  
Vijaya Bharathi Srinivasan ◽  
Vasanth Vaidyanathan ◽  
Amitabha Mondal ◽  
Manjunath Venkataramaiah ◽  
Govindan Rajamohan
Keyword(s):  
Klebsiella Pneumoniae ◽  
Functional Characterization ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Dependent Protein

Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance

Plant Science ◽  
2007 ◽  
Vol 173 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Rong-Chao Ge ◽  
Gui-Ping Chen ◽  
Bao-Cun Zhao ◽  
Yin-Zhu Shen ◽  
Zhan-Jing Huang
Keyword(s):  
Functional Characterization ◽  
Salt Resistance ◽  
Serine Threonine Kinase ◽  
Kinase Gene ◽  
Threonine Kinase

Increased amount of a 25-kilodalton phosphoprotein after v-mos transfection of CHO cells

1988 ◽  
Vol 8 (11) ◽  
pp. 4685-4691
Author(s):  
J K Mayo ◽  
K E Sampson ◽  
L D Adams ◽  
E R Crumm ◽  
S L Kelly ◽  
...  
Keyword(s):  
Growth Rate ◽  
Cho Cells ◽  
Kinase Activity ◽  
Chinese Hamster ◽  
Temperature Sensitive ◽  
Serine Threonine Kinase ◽  
Dependent Protein Kinase ◽  
Threonine Kinase ◽  
Dependent Protein ◽  
A Cell

We transfected Chinese hamster ovary (CHO) cells with a cloned v-mos gene (pHT25). The mos family of oncogenes has previously been shown to have serine-threonine kinase activity. This kinase activity may be required for oncogenic transformation, although its exact biological role is unknown. We found that the transfected cells had an altered morphology, a slower doubling time, and an apparent increase in the amount of a 25-kilodalton (kDa) phosphoprotein that appeared to be of low abundance. Transfection of CHO cells with a cloned temperature-sensitive mos gene (ts159) led to isolation of a cell line that showed the presence of the 25-kDa phosphoprotein at the permissive but not at the nonpermissive temperature, suggesting a direct relationship between mos activity and the presence of this phosphoprotein. The characteristics of altered morphology and depressed growth rate were reminiscent of changes seen after the activation of the cyclic AMP-dependent protein kinase (PKA) in CHO cells. However, PKA activation did not stimulate phosphorylation of this 25-kDa protein, nor was there a change in total PKA activity in these cells. We suggest that the increased presence of the 25-kDa phosphoprotein is a consequence of the v-mos transfection and that it may be involved in the change of morphology and growth rate seen in the CHO cells. Phosphorylation of this protein may be a useful marker of mos and have some functional importance in the transformation of cells by the v-mos oncogene.

Identification and molecular characterization of the Pim1 serine/threonine kinase homolog in Litopenaeus vannamei

2018 ◽  
Vol 74 ◽  
pp. 491-500 ◽  
Author(s):  
Jude Juventus Aweya ◽  
Wei Wang ◽  
Yueling Zhang ◽  
Defu Yao ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Litopenaeus Vannamei ◽  
Serine Threonine Kinase ◽  
Threonine Kinase

Biochemical and Crystallographic Characterization of a Rho Effector Domain of the Protein Serine/Threonine Kinase N in a Complex with RhoA

1999 ◽  
Vol 126 (2) ◽  
pp. 166-170 ◽  
Author(s):  
Ryoko Maesaki ◽  
Toshiyuki Shimizu ◽  
Kentaro Ihara ◽  
Shinya Kuroda ◽  
Kozo Kaibuchi ◽  
...  
Keyword(s):  
Serine Threonine Kinase ◽  
Effector Domain ◽  
Threonine Kinase

Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice

1994 ◽  
Vol 26 (3) ◽  
pp. 791-803 ◽  
Author(s):  
Yan Zhao ◽  
Xin-Hua Feng ◽  
John C. Watson ◽  
Paul J. Bottino ◽  
Shain-Dow Kung
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Serine Threonine Kinase ◽  
Threonine Kinase
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