scholarly journals Rational design of DNA sequence-specific zinc fingers

FEBS Letters ◽  
2012 ◽  
Vol 586 (6) ◽  
pp. 918-923 ◽  
Author(s):  
Hidetoshi Kono ◽  
Miki Imanishi ◽  
Shigeru Negi ◽  
Kazuya Tatsutani ◽  
Yui Sakaeda ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Rational Design ◽  
Zinc Fingers

scholarly journals Rational design of a DNA sequence-specific modular protein tag by tuning the alkylation kinetics

2019 ◽  
Vol 10 (40) ◽  
pp. 9315-9325 ◽  
Author(s):  
Thang Minh Nguyen ◽  
Eiji Nakata ◽  
Zhengxiao Zhang ◽  
Masayuki Saimura ◽  
Huyen Dinh ◽  
...  
Keyword(s):  
Dna Binding ◽  
Kinetic Parameters ◽  
Dna Sequence ◽  
Rational Design ◽  
Design Principle ◽  
Dna Recognition ◽  
Protein Tag

A design principle for sequence-specific DNA modifiers driven by the specific DNA recognition was proposed based on the kinetic parameters for DNA binding and modification reactions.

Solution structure of the first three zinc fingers of TFIIIA bound to the cognate DNA sequence: determinants of affinity and sequence specificity

1997 ◽  
Vol 273 (1) ◽  
pp. 183-206 ◽  
Author(s):  
Deborah S. Wuttke ◽  
Mark P. Foster ◽  
David A. Case ◽  
Joel M. Gottesfeld ◽  
Peter E. Wright
Keyword(s):  
Dna Sequence ◽  
Solution Structure ◽  
Zinc Fingers ◽  
Sequence Specificity

scholarly journals Rational Design of DNA Sequence-Based Strategies for Subtyping Listeria monocytogenes

2002 ◽  
Vol 40 (9) ◽  
pp. 3319-3325 ◽  
Author(s):  
S. Cai ◽  
D. Y. Kabuki ◽  
A. Y. Kuaye ◽  
T. G. Cargioli ◽  
M. S. Chung ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Dna Sequence ◽  
Rational Design

scholarly journals CTCF As an Example of DNA-Binding Transcription Factors Containing Clusters of C2H2-Type Zinc Fingers

Acta Naturae ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 31-46
Author(s):  
Oksana G. Maksimenko ◽  
Dariya V. Fursenko ◽  
Elena V. Belova ◽  
Pavel G. Georgiev
Keyword(s):  
Experimental Data ◽  
Transcription Factors ◽  
Dna Binding ◽  
Characteristic Feature ◽  
Dna Sequence ◽  
Binding Sites ◽  
Zinc Fingers ◽  
Topologically Associating Domains ◽  
Key Factor

In mammals, most of the boundaries of topologically associating domains and all well-studied insulators are rich in binding sites for the CTCF protein. According to existing experimental data, CTCF is a key factor in the organization of the architecture of mammalian chromosomes. A characteristic feature of the CTCF is that the central part of the protein contains a cluster consisting of eleven domains of C2H2-type zinc fingers, five of which specifically bind to a long DNA sequence conserved in most animals. The class of transcription factors that carry a cluster of C2H2-type zinc fingers consisting of five or more domains (C2H2 proteins) is widely represented in all groups of animals. The functions of most C2H2 proteins still remain unknown. This review presents data on the structure and possible functions of these proteins, using the example of the vertebrate CTCF protein and several well- characterized C2H2 proteins in Drosophila and mammals.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

Optimizing electron density of nickel sulfide electrocatalysts through sulfur vacancy engineering for alkaline hydrogen evolution

2020 ◽  
Vol 8 (35) ◽  
pp. 18207-18214
Author(s):  
Dongbo Jia ◽  
Lili Han ◽  
Ying Li ◽  
Wenjun He ◽  
Caichi Liu ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Electron Density ◽  
Rational Design ◽  
Nickel Sulfide ◽  
Catalytic Performance ◽  
Sulfide Catalysts ◽  
Sulfur Vacancy

A novel, rational design for porous S-vacancy nickel sulfide catalysts with remarkable catalytic performance for alkaline HER.

Phylogenetic relationships ofSalvia(Lamiaceae) in China: Evidence from DNA sequence datasets

2012 ◽  
pp. n/a-n/a
Author(s):  
Qian-Quan Li ◽  
Min-Hui Li ◽  
Qing-Jun Yuan ◽  
Zhan-Hu Cui ◽  
Lu-Qi Huang ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Relationships
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