Inhibition of osteoblast differentiation by aluminum trichloride exposure is associated with inhibition of BMP-2/Smad pathway component expression

2016 ◽  
Vol 97 ◽  
pp. 120-126 ◽  
Author(s):  
Xu Yang ◽  
Hui Huo ◽  
Chunyu Xiu ◽  
Miao Song ◽  
Yanfei Han ◽  
...  
Keyword(s):  
Osteoblast Differentiation ◽  
Smad Pathway ◽  
Aluminum Trichloride

Exosomal miRNA-486-5p derived from rheumatoid arthritis fibroblast-like synoviocytes induces osteoblast differentiation through the Tob1/BMP/Smad pathway

2020 ◽  
Vol 8 (12) ◽  
pp. 3430-3442
Author(s):  
Jie Chen ◽  
Mao Liu ◽  
Xiao Luo ◽  
Lihui Peng ◽  
Zixia Zhao ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Osteoblast Differentiation ◽  
Smad Pathway ◽  
Exosomal Mirna ◽  
Fibroblast Like Synoviocytes

The pathogenesis of rheumatoid arthritis (RA) is related to the inhibition of osteoblast differentiation.

scholarly journals Inhibition of BMP2-Induced Bone Formation by the p65 Subunit of NF-κB via an Interaction With Smad4

2014 ◽  
Vol 28 (9) ◽  
pp. 1460-1470 ◽  
Author(s):  
Shizu Hirata-Tsuchiya ◽  
Hidefumi Fukushima ◽  
Takenobu Katagiri ◽  
Satoshi Ohte ◽  
Masashi Shin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Bone Formation ◽  
Osteoblast Differentiation ◽  
Bone Diseases ◽  
Bmp Signaling ◽  
Wild Type ◽  
Smad Pathway ◽  
Wild Type Mefs

Bone morphogenic proteins (BMPs) stimulate bone formation in vivo and osteoblast differentiation in vitro via a Smad signaling pathway. Recent findings revealed that the activation of nuclear factor-κB (NF-κB) inhibits BMP-induced osteoblast differentiation. Here, we show that NF-κB inhibits BMP signaling by directly targeting the Smad pathway. A selective inhibitor of the classic NF-κB pathway, BAY11–770682, enhanced BMP2-induced ectopic bone formation in vivo. In mouse embryonic fibroblasts (MEFs) prepared from mice deficient in p65, the main subunit of NF-κB, BMP2, induced osteoblastic differentiation via the Smad complex to a greater extent than that in wild-type MEFs. In p65−/− MEFs, the BMP2-activated Smad complex bound much more stably to the target element than that in wild-type MEFs without affecting the phosphorylation levels of Smad1/5/8. Overexpression of p65 inhibited BMP2 activity by decreasing the DNA binding of the Smad complex. The C-terminal region, including the TA2 domain, of p65 was essential for inhibiting the BMP-Smad pathway. The C-terminal TA2 domain of p65 associated with the MH1 domain of Smad4 but not Smad1. Taken together, our results suggest that p65 inhibits BMP signaling by blocking the DNA binding of the Smad complex via an interaction with Smad4. Our study also suggests that targeting the association between p65 and Smad4 may help to promote bone regeneration in the treatment of bone diseases.

Author response for "Thrombomodulin functional domains support osteoblast differentiation and bone healing in diabetes in mice"

2020 ◽  
Author(s):  
Chung‐Hwan Chen ◽  
Chao‐Han Lai ◽  
Yi‐Kai Hong ◽  
Jui‐Ming Lu ◽  
Sung‐Yen Lin ◽  
...  
Keyword(s):  
Bone Healing ◽  
Osteoblast Differentiation ◽  
Author Response ◽  
Functional Domains ◽  
Diabetes In Mice

Osteogenic markers are decreased in SHR rats during in vitro osteoblast differentiation

2014 ◽  
Author(s):  
Barros Thamine Landim de ◽  
Antonio Chaves-Neto ◽  
Amaral Caril do ◽  
Victor Brito ◽  
Sandra Oliveira
Keyword(s):  
Osteoblast Differentiation ◽  
Shr Rats ◽  
Osteogenic Markers

Anti-Osteoactivin Antibody Inhibits Osteoblast Differentiation and Function In Vitro

2003 ◽  
Vol 13 (2-4) ◽  
pp. 12 ◽  
Author(s):  
Abdulhafez A. Selim ◽  
Samir M. Abdelmagid ◽  
Reem A. Kanaan ◽  
Steven L. Smock ◽  
Thomas A. Owen ◽  
...  
Keyword(s):  
Osteoblast Differentiation ◽  
And Function
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