scholarly journals Evaluation of food-relevant chemicals in the ToxCast high-throughput screening program

2016 ◽  
Vol 92 ◽  
pp. 188-196 ◽  
Author(s):  
Agnes L. Karmaus ◽  
Dayne L. Filer ◽  
Matthew T. Martin ◽  
Keith A. Houck
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

scholarly journals An Ultra-High Throughput Screening Approach for an Adenine Transferase Using Fluorescence Polarization

2000 ◽  
Vol 5 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Zhuyin Li ◽  
Shujaath Mehdi ◽  
Indravadan Patel ◽  
John Kawooya ◽  
Madeline Judkins ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Polarization ◽  
High Throughput Screening ◽  
Screening Program ◽  
Rotational Correlation Time ◽  
Assay Method ◽  
Long Wavelength ◽  
Spacer Arm ◽  
Texas Red ◽  
Kinetics Of

We have developed a novel assay for measuring the activity of an enzyme that transfers multiple adenine-containing groups to an acceptor protein. The assay is based on fluorescence polarization (FP) technology in a 1536-well plate format. In the assay, a long wavelength fluorescence tracer, Texas Red (Rhodamine), was covalently conjugated to adenine of the donor substrate through a C6 spacer arm. As a result of the transfer of the adenine-containing moieties to the acceptor protein substrate, the rotational correlation time of the Texas Red conjugate increased, hence increasing the degree of fluorescence polarization. The pharmacological profile and kinetics of the enzyme measured according to the FP method were consistent with those determined previously by conventional analysis. We have successfully executed a 250,000-compound high throughput screening program based on the FP assay method. The quality and validity of the assay were verified by a variety of statistical analyses.

Abstract 6338: High-throughput screening program to target ovarian cancer leader cells

2020 ◽  
Author(s):  
Nazanin Karimnia ◽  
Maree Bilandzic ◽  
Magdalena Plebanski ◽  
Andrew N. Stephens
Keyword(s):  
Ovarian Cancer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

High-throughput screening revealed a clinically relevant drug to induce sperm motility

2014 ◽  
Author(s):  
Clair Cochrane ◽  
Halil Ruso ◽  
Anthony Hope ◽  
Rosemary G Clarke ◽  
Christopher Barratt ◽  
...  
Keyword(s):  
Sperm Motility ◽  
High Throughput ◽  
High Throughput Screening
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