Radioprotection by two phenolic compounds: Chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro

2013 ◽  
Vol 53 ◽  
pp. 359-363 ◽  
Author(s):  
Nilufer Cinkilic ◽  
Sibel Kahraman Cetintas ◽  
Tolga Zorlu ◽  
Ozgur Vatan ◽  
Dilek Yilmaz ◽  
...  
Keyword(s):  
Dna Damage ◽  
Phenolic Compounds ◽  
Human Blood ◽  
Quinic Acid ◽  
X Ray ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

Potential of rod, sphere and semi-cube shaped gold nanoparticles to induce cytotoxicity and genotoxicity in human blood lymphocytes in vitro

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Mona A.M. Abo-Zeid ◽  
Thomas Liehr ◽  
Amira M. Gamal-Eldeen ◽  
Mahmoud Zawrah ◽  
Mostafa Ali ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gold Nanoparticles ◽  
Mitotic Activity ◽  
Human Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes ◽  
Different Shapes

AbstractGold nanoparticles (GNPs) are intended to be used in nanomedicine. Due to nanotechnology innovation GNPs of variable sizes and in different shapes including rods, spheres, cubes, etc., can easily be produced. The aim of the present studies was to evaluate the cyto-and genotoxicity inducible by different shaped GNPs on normal human peripheral blood lymphocytes.Four different shapes of GNPs including big rod GNPs (BR-GNPs, 50 nm), small rod GNPs (SR-GNPs, 30 nm), sphere GNPs (S-GNPs, 15 nm) and semi-cube GNPs (SC-GNPs, 15 nm) were studied. Cultured human blood lymphocytes were treated with different concentrations of these GNPs for 24 h in vitro. Cytotoxicity was evaluated based on the mitotic index (MI), while genotoxicity was studied by an interphase-fluorescence in situ hybridization (I-FISH) assay. The following genes were studied in I-FISH:The lowest concentration of BR-GNPs neither had an effect mitotic activity nor enhanced gain or loss of examined gene signals in a significant manner with I-FSH. Other concentrations of BR-GNPs, SR-GNPs, S-GNPs and SC-GNPs with all concentrations inhibited the mitotic activity of the cells and reduced the cell proliferation highly significantly. The different types of GNPs initiated the duplication ofGNPs at high concentration can reduce the cell proliferation and induce DNA damage. Low concentration of rod-shaped GNPs at 50 nm was safe on human lymphocytes. Further research studies are required to optimize the concentration, shape and size of GNPs before using them in nanomedicine.

Effect of Antioxidants on X-ray–induced γ-H2AX Foci in Human Blood Lymphocytes: Preliminary Observations

Radiology ◽  
2012 ◽  
Vol 264 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Michael A. Kuefner ◽  
Michael Brand ◽  
James Ehrlich ◽  
Larissa Braga ◽  
Michael Uder ◽  
...  
Keyword(s):  
Human Blood ◽  
X Ray ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes
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