The exposure of highly toxic aconitine does not significantly impact the activity and expression of cytochrome P450 3A in rats determined by a novel ultra performance liquid chromatography–tandem mass spectrometric method of a specific probe buspirone

2013 ◽  
Vol 51 ◽  
pp. 396-403 ◽  
Author(s):  
Lijun Zhu ◽  
Xiaoshan Yang ◽  
Juan Zhou ◽  
Lan Tang ◽  
Bijun Xia ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Specific Probe ◽  
Cytochrome P450 3A ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric

scholarly journals Ultra-performance liquid chromatography-tandem mass spectrometric method for quantitation of the recently Food and Drug Administration approved combination of vaborbactam and meropenem in human plasma

2020 ◽  
Vol 7 (7) ◽  
pp. 200635
Author(s):  
Ahmed K. Kammoun ◽  
Alaa Khedr ◽  
Ahdab N. Khayyat ◽  
Maha A. Hegazy
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Drug Administration ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric

A parenteral medical combination containing vaborbactam and meropenem is used mainly to treat complicated urinary tract infections. A novel ultra-performance liquid chromatography tandem mass spectrometric method was developed for the sensitive determination of both compounds in human plasma. Sample preparation was performed by precipitation technique. The chromatographic separation was accomplished using the Acquity C18-BEH column, 0.01 M ammonium formate: acetonitrile (47 : 53, v/v) as a mobile phase with a flow rate of 0.2 ml min −1 . Analytes were monitored by applying multiple reaction monitoring. The bioanalytical validation criteria were conducted following the Food and Drug Administration recommendations. The method was linear within range 0.5 to 50 µg ml −1 , for both drugs. The intra-day and inter-day precision, as coefficient variation (% CV) and the accuracy, as % bias did not exceed 15% for both drugs. The percentage recovery of targeted analytes was not less than 77%, calculated at three quality control levels. The proposed method showed a suitable lower level of quantification value of 0.50 µg ml −1 for both analytes, which is far lower than the expected C max , which permits the use of this method for pharmacokinetic studies. The proposed method proved to be useful for the evaluation of this combination in both human plasma and pharmaceutical formulation.

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