scholarly journals Assessment of usnic acid toxicity in rat primary hepatocytes using 13C isotopomer distribution analysis of lactate, glutamate and glucose

2011 ◽  
Vol 49 (11) ◽  
pp. 2968-2974 ◽  
Author(s):  
Bakary J. Sonko ◽  
Thomas C. Schmitt ◽  
Lei Guo ◽  
Qiang Shi ◽  
Laszlo G. Boros ◽  
...  
Keyword(s):  
Usnic Acid ◽  
Distribution Analysis ◽  
Primary Hepatocytes ◽  
Isotopomer Distribution ◽  
Rat Primary Hepatocytes ◽  
Acid Toxicity

13C-NMR isotopomer distribution analysis: a method for measuring metabolic fluxes in condensation biosynthesis

2002 ◽  
Vol 15 (6) ◽  
pp. 404-415 ◽  
Author(s):  
Caterina Puccetti ◽  
Tommaso Aureli ◽  
Cesare Manetti ◽  
Filippo Conti
Keyword(s):  
13C Nmr ◽  
Distribution Analysis ◽  
Isotopomer Distribution ◽  
Metabolic Fluxes

Butein Up-Regulates the Expression of the π Class of GlutathioneS-Transferase in Rat Primary Hepatocytes through the ERK/AP-1 Pathway

2010 ◽  
Vol 58 (16) ◽  
pp. 8994-9000 ◽  
Author(s):  
Hebron C. Chang ◽  
Haw-Wen Chen ◽  
Hui-Shan Tung ◽  
Kai-Li Liu ◽  
Chia-Wen Tsai ◽  
...  
Keyword(s):  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes

scholarly journals Software LS-MIDA for efficient mass isotopomer distribution analysis in metabolic modelling

2013 ◽  
Vol 14 (1) ◽  
pp. 218 ◽  
Author(s):  
Zeeshan Ahmed ◽  
Saman Zeeshan ◽  
Claudia Huber ◽  
Michael Hensel ◽  
Dietmar Schomburg ◽  
...  
Keyword(s):  
Distribution Analysis ◽  
Metabolic Modelling ◽  
Isotopomer Distribution ◽  
Mass Isotopomer Distribution Analysis ◽  
Mass Isotopomer

Measurement of gluconeogenesis in exercising men by mass isotopomer distribution analysis

2002 ◽  
Vol 93 (1) ◽  
pp. 233-241 ◽  
Author(s):  
Jeff K. Trimmer ◽  
Jean-Marc Schwarz ◽  
Gretchen A. Casazza ◽  
Michael A. Horning ◽  
Nestor Rodriguez ◽  
...  
Keyword(s):  
Rest Period ◽  
Glucose Production ◽  
Moderate Intensity ◽  
Dependent Manner ◽  
Distribution Analysis ◽  
Moderate Intensity Exercise ◽  
Isotopomer Distribution ◽  
Mass Isotopomer Distribution Analysis ◽  
The Difference ◽  
Mass Isotopomer

We evaluated the hypothesis that coordinated adjustments in absolute rates of gluconeogenesis (GNGab) and hepatic glycogenolysis (Gly) would maintain euglycemia and match glucose production (GP) to peripheral utilization during rest and exercise. Specifically, we evaluated the extent to which gradations in exercise power output would affect the contribution of GNGab to GP. For these purposes, we employed mass isotopomer distribution analysis (MIDA) and isotope-dilution techniques on eight postabsorptive (PA) endurance-trained men during 90 min of leg cycle ergometry at 45 and 65% peak O2 consumption (V˙o 2 peak; moderate and hard intensities, respectively) and the preceding rest period. GP was constant in resting subjects, whereas the fraction from GNG (fGNG) increased over time during rest (22.3 ± 0.9% at 11.25 h PA vs. 25.6 ± 0.9% at 12.0 h PA, P < 0.05). In the transition from rest to exercise, GP increased in an intensity-dependent manner (rest, 2.0 ± 0.1; 45%, 4.0 ± 0.4; 65%, 5.84 ± 0.64 mg · kg−1 · min−1, P < 0.05), although glucose rate of disappearance exceeded rate of appearance during the last 30 min of exercise at 65%V˙o 2 peak. Compared with rest, increases in GP were sustained by 92 and 135% increments in GNGab during moderate- and hard-intensity exercises, respectively. Correspondingly, Gly (calculated as the difference between GP and MIDA-measured GNGab) increased 100 and 203% over rest during the two exercise intensities. During moderate-intensity exercise, fGNG was the same as at rest; however, during the harder exercise fGNG decreased significantly to account for only 21% of GP. The highest sustained GNGab observed in these trials on PA men was 1.24 ± 0.3 mg · kg−1 · min−1. We conclude that, after an overnight fast, 1) absolute GNG rates increased with intensity of effort despite a reduced fGNG at 65% V˙o 2 peak, 2) during exercise Gly is more responsible than GNGab for maintaining GP, and 3) in 12-h fasted men, neither increased Gly or GNGab nor was their combination able to maintain euglycemia during prolonged hard (65%V˙o 2 peak) exercise.

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