Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronically or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma proteins in the pig

2006 ◽  
Vol 44 (12) ◽  
pp. 1953-1965 ◽  
Author(s):  
Tanja Goyarts ◽  
Nicola Grove ◽  
Sven Dänicke
Keyword(s):  
Protein Synthesis ◽  
Single Dose ◽  
Peripheral Blood ◽  
Plasma Proteins ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Fusarium Toxin ◽  
In Vivo Protein Synthesis

Determination of Protein Synthesis in Lymphocytes in Vivo after Surgery

1996 ◽  
Vol 91 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Pia Essén ◽  
Margaret A. McNurlan ◽  
Anders Thorell ◽  
Inga Tjäder ◽  
Giuseppe Caso ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Mixed Population ◽  
Synthesis Rate ◽  
Blood Lymphocytes ◽  
Mononuclear Leucocytes

1. The stimulation and depression of peripheral blood lymphocytes has previously been studied in vitro, showing an immune depression postoperatively; however, it is difficult to interpret these in vitro findings. Therefore, an in vivo technique has been established for determination of the fractional protein synthesis rate, as an index of metabolic activity in human peripheral blood lymphocytes, by using a stable isotope technique. 2. The rate of protein synthesis was calculated from the increase in enrichment of l-[2H5]phenylalanine in protein of a mixed population of mononuclear leucocytes, isolated by density gradient, after an intravenous flooding dose of l-[2H5]phenylalanine. A linear time course of isotopic incorporation into the cells was demonstrated. 3. The fractional rate of protein synthesis of a mixed population of mononuclear leucocytes was studied in relation to surgical interventions and to potential modifiers of the response. The fractional synthesis rate increased 24 h after open and laparoscopic cholecystectomy (49 ± 19% and 40 ± 14% respectively, P > 0.02), irrespective of postoperative total parenteral nutrition or preoperative glucose infusion. In contrast to surgery, insulin did not stimulate protein synthesis in peripheral mononuclear leucocytes.

scholarly journals Hepadnavirus infection of peripheral blood lymphocytes in vivo: woodchuck and chimpanzee models of viral hepatitis.

1986 ◽  
Vol 58 (1) ◽  
pp. 1-8 ◽  
Author(s):  
B E Korba ◽  
F Wells ◽  
B C Tennant ◽  
G H Yoakum ◽  
R H Purcell ◽  
...  
Keyword(s):  
Viral Hepatitis ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

EFFECT OF l-CARNITINE TREATMENT IN VIVO ON APOPTOSIS AND CERAMIDE GENERATION IN PERIPHERAL BLOOD LYMPHOCYTES FROM AIDS PATIENTS: CORRELATION WITH IN VITRO RESULTS

1996 ◽  
Vol 24 (4) ◽  
pp. 618S-618S ◽  
Author(s):  
M. Grazia Cifone ◽  
Edoardo Alesse ◽  
Luisa Di Marzio ◽  
Paola Roncaioli ◽  
Francesca Zazzeroni ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Aids Patients ◽  
Blood Lymphocytes ◽  
Ceramide Generation

Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes

Blood ◽  
2000 ◽  
Vol 96 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Matthew Sharron ◽  
Stefan Pöhlmann ◽  
Ken Price ◽  
Elias Lolis ◽  
Monica Tsang ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Expression Pattern ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Immunodeficiency Virus ◽  
Hiv 1

Abstract CCR5 and CXCR4 are the major coreceptors that mediate human immunodeficiency virus 1 (HIV-1) infection, while most simian immunodeficiency virus (SIV) isolates use CCR5. A number of alternative coreceptors can also mediate infection of some virus strains in vitro, although little is known about their in vivo relevance. Therefore, we characterized the expression pattern and coreceptor activity of one of these alternative coreceptors, STRL33/Bonzo, using a newly developed monoclonal antibody. In addition to being highly expressed (approximately 1000-7000 STRL33 ABS [antibody binding sites]) on specific subsets of natural killer cells (CD3−/CD16−/low/CD56+ and CD3−/CD16low/CD56−) and CD19+ B lymphocytes (approximately 300-5000 STRL33 ABS), STRL33 was expressed at levels sufficient to support virus infection on freshly isolated, truly naive CD4+/CD45RA+/CD62L+cells (6000-11 000 ABS). STRL33 expression on peripheral blood mononuclear cells (PBMCs) was increased by mitogenic stimulation (OKT3/IL-2 [interleukin-2] had a greater effect than phytohemaglutinin (PHA)/IL-2), but it was dramatically decreased upon Ficoll purification. Infection of CCR5− human peripheral blood lymphocytes (PBLs) showed that 2 different SIV envelope (Env) proteins mediated entry into STRL33+cells. More importantly, the preferential infection of STRL33+ cells in CCR5− PBLs by an R5/X4/STRL33 HIV-1 maternal isolate in the presence of a potent CXCR4 antagonist (AMD3100) suggests that STRL33 can be used as a coreceptor by HIV-1 on primary cells. Rhesus macaque (rh) STRL33 was used less efficiently than human STRL33 by the majority of SIV Env proteins tested despite similar levels of expression, thereby making it less likely that STRL33 is a relevant coreceptor in the rhesus macaque system. In summary, the expression pattern and coreceptor activity of STRL33 suggest its involvement in trafficking of tumor-infiltrating lymphocytes and indicate that STRL33 may be a relevant coreceptor in vivo.

Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes

Blood ◽  
2000 ◽  
Vol 96 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Matthew Sharron ◽  
Stefan Pöhlmann ◽  
Ken Price ◽  
Elias Lolis ◽  
Monica Tsang ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Expression Pattern ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Immunodeficiency Virus ◽  
Hiv 1

CCR5 and CXCR4 are the major coreceptors that mediate human immunodeficiency virus 1 (HIV-1) infection, while most simian immunodeficiency virus (SIV) isolates use CCR5. A number of alternative coreceptors can also mediate infection of some virus strains in vitro, although little is known about their in vivo relevance. Therefore, we characterized the expression pattern and coreceptor activity of one of these alternative coreceptors, STRL33/Bonzo, using a newly developed monoclonal antibody. In addition to being highly expressed (approximately 1000-7000 STRL33 ABS [antibody binding sites]) on specific subsets of natural killer cells (CD3−/CD16−/low/CD56+ and CD3−/CD16low/CD56−) and CD19+ B lymphocytes (approximately 300-5000 STRL33 ABS), STRL33 was expressed at levels sufficient to support virus infection on freshly isolated, truly naive CD4+/CD45RA+/CD62L+cells (6000-11 000 ABS). STRL33 expression on peripheral blood mononuclear cells (PBMCs) was increased by mitogenic stimulation (OKT3/IL-2 [interleukin-2] had a greater effect than phytohemaglutinin (PHA)/IL-2), but it was dramatically decreased upon Ficoll purification. Infection of CCR5− human peripheral blood lymphocytes (PBLs) showed that 2 different SIV envelope (Env) proteins mediated entry into STRL33+cells. More importantly, the preferential infection of STRL33+ cells in CCR5− PBLs by an R5/X4/STRL33 HIV-1 maternal isolate in the presence of a potent CXCR4 antagonist (AMD3100) suggests that STRL33 can be used as a coreceptor by HIV-1 on primary cells. Rhesus macaque (rh) STRL33 was used less efficiently than human STRL33 by the majority of SIV Env proteins tested despite similar levels of expression, thereby making it less likely that STRL33 is a relevant coreceptor in the rhesus macaque system. In summary, the expression pattern and coreceptor activity of STRL33 suggest its involvement in trafficking of tumor-infiltrating lymphocytes and indicate that STRL33 may be a relevant coreceptor in vivo.

In vivo amifostine (WR-2721) prevents chemotherapy-induced apoptosis of peripheral blood lymphocytes from cancer patients

Life Sciences ◽  
1999 ◽  
Vol 64 (17) ◽  
pp. 1525-1532 ◽  
Author(s):  
Mauro Provinciali ◽  
Andrea Ciavattini ◽  
Giuseppina Di Stefano ◽  
Katy Argentati ◽  
Giuseppe Gioele Garzetti
Keyword(s):  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Induced Apoptosis
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