scholarly journals Effects of amitraz on cytochrome P450-dependent monooxygenases and estrogenic activity in MCF-7 human breast cancer cells and immature female rats

2004 ◽  
Vol 42 (11) ◽  
pp. 1785-1794 ◽  
Author(s):  
Tzuu-Huei Ueng ◽  
Chia-Chi Hung ◽  
Hui-Wu Wang ◽  
Ping-Kun Chan
Keyword(s):  
Breast Cancer ◽  
Cytochrome P450 ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Estrogenic Activity ◽  
Female Rats ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Immature Female ◽  
Mcf 7

Estrogen Activities and the Cellular Effects of Natural Progesterone from Wild Yam Extract in MCF-7 Human Breast Cancer Cells

2009 ◽  
Vol 37 (01) ◽  
pp. 159-167 ◽  
Author(s):  
Mi-Kyung Park ◽  
Hyeok-Yi Kwon ◽  
Woong-Shick Ahn ◽  
Sumi Bae ◽  
Mee-Ra Rhyu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Estrogenic Activity ◽  
Mrna Levels ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Wild Yam ◽  
Mcf 7

We studied the estrogenic activity and cellular effect of wild yam extract in MCF-7 human breast cancer cells. The extract increased the activity of the progesterone receptor and pS2 genes at the mRNA levels in human breast cancer MCF-7 cells, although the effects were not as prominent as those of 17β-estradiol (E2). Western blot analysis showed that the level of estrogen receptor α protein was down-regulated after treatment with E2 or wild yam extract. Wild yam extract also inhibited proliferation of MCF-7 cells. These data indicate that wild yam extract acts as a weak phytoestrogen and protects against proliferation in human breast carcinoma MCF-7 cells.

scholarly journals Transcriptome profiling reveals bisphenol A alternatives activate estrogen receptor alpha in human breast cancer cells

2017 ◽  
Author(s):  
Robin Mesnage ◽  
Alexia Phedonos ◽  
Matthew Arno ◽  
Sucharitha Balu ◽  
J. Christopher Corton ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Estrogen Receptor ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Estrogenic Activity ◽  
Human Breast Cancer Cell ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

AbstractBackgroundPlasticizers with estrogenic activity, such as bisphenol A (BPA), have been reported to have potential adverse health effects in humans. Due to mounting evidence of these health effects and public pressure, BPA is being phased out by the plastics manufacturing industry and replaced by other bisphenol variants in “BPA-free” products.ObjectivesWe have compared estrogenic activity of BPA to 6 bisphenol analogues (bisphenol S, BPS; bisphenol F, BPF; bisphenol AP, BPAP; bisphenol AF, BPAF; bisphenol Z, BPZ; bisphenol B, BPB) in three human breast cancer cell lines.MethodsEstrogenicity was assessed by cell growth in an estrogen receptor (ER)-mediated cell proliferation assay, and by the induction of estrogen response element (ERE)-mediated transcription in a luciferase assay. Gene expression profiles were determined in MCF-7 human breast cancer cells by microarray analysis and confirmed by Illumina-based RNA sequencing.ResultsAll bisphenols showed estrogenic activity in promoting cell growth and inducing ERE-mediated transcription. BPAF was the most potent bisphenol, followed by BPB > BPZ ~ BPA > BPF ~ BPAP > BPS. The addition of ICI 182,780 antagonized the activation of ERs by bisphenols. Data mining of ToxCast high-throughput screening assays confirms our results but also shows divergence in the sensitivities of the assays. The comparison of transcriptome profile alterations resulting from BPA alternatives with an ERα gene expression biomarker further indicates that all BPA alternatives act as ERα agonists in MCF-7 cells. These results were confirmed by RNA sequencing.ConclusionIn conclusion, BPA alternatives are not necessarily less estrogenic in a human breast cancer cell model. Three bisphenols (BPAF, BPB, and BPZ) were more estrogenic than BPA. The relevance of human exposure to BPA alternatives in hormone-dependent breast cancer risk should be investigated.

Novel antioestrogens

1989 ◽  
Vol 95 ◽  
pp. 247-252 ◽  
Author(s):  
A. E. Wakeling ◽  
J. Bowler
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Partial Agonist ◽  
Female Rats ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Intact Female ◽  
Lh Secretion ◽  
Mcf 7

SynopsisRecently described 7α-alkylamide analogues of oestradiol, exemplified by ICI 164,384, have the pharmacological characteristics of pure antioestrogens. ICI 164,384 is completely free of oestrogenic activity in rodents and blocks the trophic actions of exogenous and endogenous oestradiol and of partial agonist antiooestrogens like tamoxifen. In addition, ICI 164,384 is peripherally selective, producing complete involution of the uterus without affecting LH secretion in intact female rats.In MCF-7 oestrogen-responsive, human breast cancer cells ICI 164,384 is a more potent and effective inhibitor of cell growth than tamoxifen. This difference in efficacy is reflected by a greater reduction in the proportion of cells continuing DNA synthesis in ICI 164,384-treated cultures than in tamoxifen-treated cells. This observation implies that a pure antioestrogen may provide more effective therapy of breast cancer but this will have to be demonstrated in clinical studies.

scholarly journals Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor

Planta Medica ◽  
2016 ◽  
Vol 82 (16) ◽  
pp. 1425-1430 ◽  
Author(s):  
Joseph Kwon ◽  
Kyung Oh ◽  
Se-Young Cho ◽  
Mi Bang ◽  
Hwan Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Estrogenic Activity ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7
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