Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding

2012 ◽  
Vol 128 ◽  
pp. 8-16 ◽  
Author(s):  
Vikas K. Singh ◽  
Atul Singh ◽  
S.P. Singh ◽  
Ranjith K. Ellur ◽  
Vikas Choudhary ◽  
...  
Keyword(s):  
Blast Resistance ◽  
Basmati Rice ◽  
Restorer Line ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

Marker-assisted simultaneous but stepwise backcross breeding for pyramiding blast resistance genesPiz5andPi54into an elite Basmati rice restorer line ‘PRR78’

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Vikas K. Singh ◽  
Atul Singh ◽  
S. P. Singh ◽  
Ranjith K. Ellur ◽  
Devinder Singh ◽  
...  
Keyword(s):  
Blast Resistance ◽  
Basmati Rice ◽  
Restorer Line ◽  
Backcross Breeding

scholarly journals Recovery of Recurrent Parent Genome in a Marker-Assisted Backcrossing Against Rice Blast and Blight Infections Using Functional Markers and SSRs

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1411
Author(s):  
Samuel Chibuike Chukwu ◽  
Mohd Y. Rafii ◽  
Shairul Izan Ramlee ◽  
Siti Izera Ismail ◽  
Yusuff Oladosu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Blast Resistance ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Functional Markers ◽  
Recurrent Parent ◽  
Selected Lines ◽  
Recurrent Parent Genome ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

The most vital aspect of marker-assisted backcross breeding is the recurrent parent genome recovery. This enables the selection of only parents with recovered recipient/recurrent parent genome in addition to the targeted genes. The recurrent parent genome recovery (RPGR) ensures that non-desirable genomic segments are removed while the gene of interest is sustained in the recombined progenies without further segregations. This work was aimed at quantifying the RPGR of backcross populations with introgression of bacterial leaf blight resistance genes. Putra-1, a Malaysian elite variety, high yielding with inherent resistance to blast but susceptible to bacterial leaf blight (BLB), was crossed with IRBB60 which is resistant to BLB disease. The IRBB60 has four Xoo resistance genes—Xa4, xa5, xa13 and Xa21. Tightly linked polymorphic functional and SSR markers were used for foreground selection at every stage of backcrossing to select progenies with introgressed target genes. Background selection was done to quantify the percentage of RPGR in the selected lines using 79 confirmed polymorphic microsatellites. Result obtained showed that the percentage of RPGR was 80.11% at BC1F1, 95.30% at BC2F1 and 95.9% at BC2F2. The introgression of Xa4, xa5, xa13 and Xa21 Xoo resistance genes were faster through the adopted marker-assisted backcross breeding compared to what could be obtained through conventional breeding. All the 16 selected lines displayed resistance to BLB with three lines showing high resistance to the disease. The blast resistance contained in the genetic background of Putra-1 was also sustained in all the selected lines. The newly developed lines were recommended as new rice varieties for commercial cultivation.

scholarly journals Enhancement of Panicle Blast Resistance in Korean Rice Cultivar ‘Saeilmi’ by Marker Assisted Backcross Breeding

2015 ◽  
Vol 3 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Jong-Hee Lee ◽  
Ji-Yoon Lee ◽  
Young-Nam Yoon ◽  
Sang-Yeol Kim ◽  
Yeon-Jae Hur ◽  
...  
Keyword(s):  
Blast Resistance ◽  
Rice Cultivar ◽  
Panicle Blast ◽  
Backcross Breeding ◽  
Korean Rice ◽  
Marker Assisted Backcross Breeding

scholarly journals Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 202
Author(s):  
Gaurav Dhawan ◽  
Aruna Kumar ◽  
Priyanka Dwivedi ◽  
Subbaiyan Gopala Krishnan ◽  
Madan Pal ◽  
...  
Keyword(s):  
Drought Stress ◽  
Reproductive Stage ◽  
Chromosome 1 ◽  
Basmati Rice ◽  
Yield Potential ◽  
Yield Reduction ◽  
Recurrent Parent ◽  
Backcross Breeding ◽  
Selection For ◽  
Marker Assisted Backcross Breeding

Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar “Pusa Basmati 1 (PB1)” is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), “qDTY1.1” into PB1. The QTL was sourced from an aus variety, Nagina 22 (N22). A microsatellite (simple sequence repeat (SSR)) marker “RM 431” located at telomeric end (38.89 mb) of chromosome 1, and located within a 1.04 mb QTL region was employed for foreground selection for qDTY1.1 in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher recurrent parent genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking quality traits. The NILs were evaluated for three consecutive Kharif seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that P1882-12-111-3, P1882-12-111-5, P1882-12-111-6, P1882-12-111-7, P1882-12-111-12, P1882-12-111-15 and P1882-12-111-17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments.

Introgression of multiple disease resistance into a maintainer of Basmati rice CMS line by marker assisted backcross breeding

Euphytica ◽  
2014 ◽  
Vol 203 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Ashok K. Singh ◽  
Vikas K. Singh ◽  
Atul Singh ◽  
Ranjith K. Ellur ◽  
R. T. P. Pandian ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Basmati Rice ◽  
Cms Line ◽  
Multiple Disease Resistance ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43

Euphytica ◽  
2014 ◽  
Vol 200 (1) ◽  
pp. 61-77 ◽  
Author(s):  
Balakrishnan Divya ◽  
S. Robin ◽  
R. Rabindran ◽  
S. Senthil ◽  
M. Raveendran ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Blast Resistance ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

scholarly journals Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance Into an Elite Rice Variety ‘Pusa Basmati 1’ Through Marker Assisted Backcross Breeding

2020 ◽  
Author(s):  
Gaurav Dhawan ◽  
Aruna Kumar ◽  
Priyanka Dwivedi ◽  
Subbaiyan Gopala Krishnan ◽  
Madan Pal ◽  
...  
Keyword(s):  
Drought Stress ◽  
Rice Variety ◽  
Reproductive Stage ◽  
Basmati Rice ◽  
Yield Potential ◽  
High Yield ◽  
Yield Reduction ◽  
Backcross Breeding ◽  
Selection For ◽  
Marker Assisted Backcross Breeding

Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar ‘Pusa Basmati 1 (PB1)’ is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), ‘qDTY1.1’ into PB1. The QTL donor was sourced from an aus variety, Nagina 22 (N22). A QTL linked microsatellite (SSR) marker ‘RM 431’ was employed for foreground selection for qDTY1.1 in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking superiority traits. The NILs were evaluated for three consecutive Kharif seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that NIL3, NIL5, NIL6, NIL7, NIL12, NIL15 and NIL17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments.

scholarly journals Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

Plant Science ◽  
2016 ◽  
Vol 242 ◽  
pp. 330-341 ◽  
Author(s):  
Ranjith K. Ellur ◽  
Apurva Khanna ◽  
Ashutosh Yadav ◽  
Sandeep Pathania ◽  
H. Rajashekara ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Basmati Rice ◽  
Rice Varieties ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding
Sign in / Sign up

Export Citation Format

Share Document