Immobilization of pectinase from Aspergillus aculeatus in alginate beads and clarification of apple and umbu juices in a packed bed reactor

2018 ◽  
Vol 109 ◽  
pp. 9-18 ◽  
Author(s):  
Rodrigo Lira de Oliveira ◽  
Jônatas Lopes Dias ◽  
Osmar Soares da Silva ◽  
Tatiana Souza Porto
Keyword(s):  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Aspergillus Aculeatus

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

Immobilized Whole Cells as Effective Catalysts for Chiral Alcohol Production

2009 ◽  
Vol 62 (9) ◽  
pp. 1034 ◽  
Author(s):  
Jeck Fei Ng ◽  
Stephan Jaenicke
Keyword(s):  
Flow Reactor ◽  
Batch Reactor ◽  
Enantiomeric Excess ◽  
Lactobacillus Brevis ◽  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Process Conditions ◽  
Alcohol Production ◽  
Whole Cells

Recombinant Escherichia coli overexpressing the gene LbADH, which encodes for an alcohol dehydrogenase from Lactobacillus brevis, was successfully transformed and cultured. The cells are able to catalyze the reduction of pro-chiral ketones, e.g. ethyl acetoacetate into R-(–)ethyl hydroxybutyrate (EHB) with high conversion and enantiomeric excess >99%. Immobilizing the whole cells in alginate beads leads to a catalyst with improved stability and ease of handling while maintaining the high activity of the free cells. The whole-cell catalyst was tested in a stirred batch reactor (CSTR) and in a continuously operated packed-bed reactor. An Mg2+ concentration of 2 mM was crucial for maintaining the activity of the biocatalyst. After a partial optimization of the process conditions, a productivity of 1.4 gEHB gwcw–1 h–1 could be maintained in a continuous flow reactor over a prolonged period of time.

scholarly journals Efficient production of α-keto acids by immobilized E. coli -pETduet-1- Pmi LAAO in a jacketed packed-bed reactor

2019 ◽  
Vol 6 (4) ◽  
pp. 182035 ◽  
Author(s):  
Licheng Wu ◽  
Xiaolei Guo ◽  
Gaobing Wu ◽  
Pengfu Liu ◽  
Ziduo Liu
Keyword(s):  
Amino Acid ◽  
Continuous Production ◽  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Efficient Production ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
E Coli ◽  
Keto Acids

α-keto acids are compounds of primary interest for the fine chemical, pharmaceutical and agrochemical sectors. l -amino acid oxidases as an efficient tool are used for α-keto acids preparation in this study. Firstly, an l -amino acid oxidase ( Pmi LAAO) from Proteus mirabilis was discovered by data mining . Secondly, by gene expression vector screening, pETDuet-1- Pmi LAAO activity improved by 130%, as compared to the pET20b- Pmi LAAO. Pmi LAAO production was increased to 9.8 U ml −1 by optimized expression condition (OD 600 = 0.65, 0.45 mmol l −1 IPTG, 20 h of induction). Furthermore, The Pmi LAAO was stabile in the pH range of 4.0–9.0 and in the temperature range of 10–40°C; the optimal pH and temperature of recombinant Pmi LAAO were 6.5 and 37°C, respectively. Afterwards, in order to simplify product separation process, E. coli -pETduet-1- Pmi LAAO was immobilized in Ca-alginate beads. Continuous production of 2-oxo-3-phenylpropanoic acid was conducted in a packed-bed reactor via immobilized E. coli -pETduet-1- Pmi LAAO. Significantly, 29.66 g l −1 2-oxo-3-phenylpropanoic acid with a substrate conversion rate of 99.5% was achieved by correspondingly increasing the residence time (25 h). This method holds the potential to be used for efficiently producing pure α-keto acids.

Bioremediation

2020 ◽  
pp. 294-317
Author(s):  
Sneha Unnikrishnan ◽  
Nagamani Bora ◽  
Karthikeyan Ramalingam
Keyword(s):  
Large Scale ◽  
Dye Decolorization ◽  
Packed Bed ◽  
High Energy ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Textile Dye ◽  
Alternative Technologies ◽  
The Stability ◽  
Immobilization Techniques

Synthetic dyes are extensively used in several industries and the dyes are great concern for the ecosystem. During the dyeing process, a certain percentage of the used dye is released into the wastewater, causing severe environmental and health hazards. The physiochemical methods are not sustainable due to high-cost, high-energy requirements and hazardous by-products. Among all the alternative technologies to conventional wastewater treatment, bioremediation has emerged as the most desirable approach to clean up the environment and to restore its original status. The ability of microbes in decolorizing the textile effluents is significant. The decolorization can be further enhanced using immobilization techniques. Immobilization increases the stability and reusability of the microorganisms. The microorganisms can be entrapped in calcium alginate beads, that can be used to construct a packed bed reactor in which dye decolorization can be carried out on a large scale. Thus, bioremediation serves as an effective, eco-friendly solution for the pollution caused by textile dye effluents.

Continuous production of a chiral amine in a packed bed reactor with co-immobilized amine dehydrogenase and formate dehydrogenase

2021 ◽  
Vol 407 ◽  
pp. 127065
Author(s):  
Robert D. Franklin ◽  
Joshua A. Whitley ◽  
Adam A. Caparco ◽  
Bettina R. Bommarius ◽  
Julie A. Champion ◽  
...  
Keyword(s):  
Formate Dehydrogenase ◽  
Continuous Production ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Chiral Amine ◽  
Amine Dehydrogenase
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