Driving forces for chymosin partitioning on the macromolecule-salt aqueous two phase system

2016 ◽  
Vol 100 ◽  
pp. 361-371 ◽  
Author(s):  
Andrés F. Chamorro Rengifo ◽  
Gabriel M. Dias Ferreira ◽  
Guilherme M. Dias Ferreira ◽  
Maria C. Hespanhol da Silva ◽  
Jaqueline de Paula Rezende ◽  
...  
Keyword(s):  
Driving Forces ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Contribution of different chemical groups to the driving forces for the partition of phenylmethane dyes in the PEO1500 + MgSO4 + H2O aqueous two-phase system

2020 ◽  
Vol 508 ◽  
pp. 112451
Author(s):  
Elkin Dario C. Castrillon ◽  
Yara L. Coelho ◽  
Álvaro Javier P. Agudelo ◽  
Isabela A. Marques ◽  
Eliara A. Hudson ◽  
...  
Keyword(s):  
Driving Forces ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Chemical Groups

scholarly journals Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 618
Author(s):  
Yifeng Xu ◽  
Quanfu Wang ◽  
Yanhua Hou
Keyword(s):  
Driving Forces ◽  
Choline Chloride ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Porphyra Yezoensis ◽  
Deep Eutectic Solvents ◽  
Phase System ◽  
Two Phase ◽  
Native Page ◽  
Aqueous Two Phase System

R-phycoerythrin (R-PE), a marine bioactive protein, is abundant in Porphyra yezoensis with high protein content. In this study, R-PE was purified using a deep eutectic solvents aqueous two-phase system (DES-ATPS), combined with ammonium sulphate precipitation, and characterized by certain techniques. Firstly, choline chloride-urea (ChCl-U) was selected as the suitable DES to form ATPS for R-PE extraction. Then, single-factor experiments were conducted: the purity (A565/A280) of R-PE was 3.825, and the yield was 69.99% (w/w) under optimal conditions (adding 0.040 mg R-PE to ChCl-U (0.35 g)/K2HPO4 (0.8 g/mL, 0.5 mL) and extracting for 20 min). The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results revealed that the purified R-PE contained three main bands. One band was presented after purification in native-PAGE. The UV-vis spectra showed characteristic absorption peaks at 495, 540, and 565 nm. R-PE displayed an emission wavelength at 570 nm when excited at 495 nm. All spectra results illustrated that the structure of R-PE remained unchanged throughout the process, proving the effectiveness of this method. Transmission electron microscope (TEM) showed that aggregation and surrounding phenomena were the driving forces for R-PE extraction. This study could provide a green and simple purification method of R-PE in drug development.

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Continuous Purification of an Enveloped and Non-enveloped Viral Particle Using an Aqueous Two-Phase System

2021 ◽  
pp. 118753
Author(s):  
Dylan G. Turpeinen ◽  
Pratik U. Joshi ◽  
Seth A. Kriz ◽  
Supreet Kaur ◽  
Natalie M. Nold ◽  
...  
Keyword(s):  
Viral Particle ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

2015 ◽  
Vol 95 ◽  
pp. 118-124 ◽  
Author(s):  
Angélica Ribeiro da Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luísa Alexandre Ferreira ◽  
João Carlos Marcos ◽  
Igor José Boggione Santos ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Sodium Citrate ◽  
Bovine Lactoferrin ◽  
Phase System ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Poly Ethylene
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