Characterization of in vitro migratory properties of anti-CD19 chimeric receptor-redirected CIK cells for their potential use in B-ALL immunotherapy

2006 ◽  
Vol 34 (9) ◽  
pp. 1218-1228 ◽  
Author(s):  
Virna Marin ◽  
Erica Dander ◽  
Ettore Biagi ◽  
Martino Introna ◽  
Grazia Fazio ◽  
...  
Keyword(s):  
Chimeric Receptor ◽  
Cik Cells ◽  
Potential Use

scholarly journals In vitro cytotoxicity of Aspilia pluriseta Schweinf. extract fractions

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sospeter N. Njeru ◽  
Jackson M. Muema
Keyword(s):  
Biological Activities ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Root Extract ◽  
Lack Of Information ◽  
Information Gap ◽  
Diphenyltetrazolium Bromide ◽  
Potential Use

Abstract Objectives We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on its cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemical characterization of A. pluriseta root extracts, here we report on cytotoxicity of tested solvent fractions. We evaluated the potential cytotoxicity of these root extract fractions on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results We show that all solvent extract fractions (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivities are within the acceptable cytotoxicity and selective index limits. This finding scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.

scholarly journals Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-κB and AP-1 Activities In Vitro

2020 ◽  
Vol 21 (14) ◽  
pp. 4897
Author(s):  
Gregorio Peron ◽  
Jan Hošek ◽  
Ganga Prasad Phuyal ◽  
Dharma Raj Kandel ◽  
Rameshwar Adhikari ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Inflammatory Diseases ◽  
Full Potential ◽  
Phytochemical Screening ◽  
Cytotoxic Compounds ◽  
Anti Inflammatory ◽  
Potential Use ◽  
Comprehensive Characterization

Here we report the comprehensive characterization of the secondary metabolites from the leaves of Colebrookea oppositifolia Smith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MSn and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-κB and AP-1 inhibitory activity of C. oppositifolia extract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate that C. oppositifolia leaves extract could significantly reduce the viability of THP-1 cells (IC50 = 6.2 ± 1.2 µg/mL), as well as the activation of both NF-κB and AP-1 at the concentration of 2 μg/mL. Our results indicate that Nepalese C. oppositifolia is a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses of C. oppositifolia in Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.

Preparation and Characterization of Akermanite/Merwinite Scaffolds for Bone Tissue Repair

2020 ◽  
Vol 44 ◽  
pp. 73-81
Author(s):  
Mojtaba Ansari ◽  
Farzad Malmir ◽  
Amir Salati
Keyword(s):  
Bone Tissue ◽  
Bone Marrow Stromal Cells ◽  
Sol Gel ◽  
Magnesium Silicate ◽  
X Ray Diffraction ◽  
X Ray ◽  
Calcium Magnesium ◽  
Potential Use

The ceramics in the system CaO–MgO–SiO2 has recently attracted a great deal of attention because they display a good in vitro bioactivity and have potential use as bone implants. Biphasic calcium-magnesium-silicate ceramics were prepared by a sol-gel method. The dried gel with chemical composition 3CaO.MgO.2SiO2 was thermally treated at 1200 °C for 2 hrs. The structural behavior of the synthesized ceramics was examined by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Merwinite crystalline phase and akermanite phase were recognized. Then, porous akermanite/merwinite scaffolds were prepared to utilize polymer sponge method and evaluated by employing SEM. Furthermore, bone marrow stromal cells (BMSC) adhesion and proliferation on the scaffolds were evaluated by MTT assay test. Differentiation of the cells was assessed by measuring alkaline phosphatase (ALP) activity. The results demonstrated that BMSC adhered and spread well on akermanite scaffolds and proliferated with the increase in the culture time, and the differentiation rate of osteoblasts on scaffolds was comparable to that on blank culture plate control. Thus, the obtained results presented that the akermanite/merwinite scaffolds deserve attention for bone tissue engineering applications.

scholarly journals Delivery of the Radionuclide 131I Using Cationic Fusogenic Liposomes as Nanocarriers

2021 ◽  
Vol 22 (1) ◽  
pp. 457
Author(s):  
Rejhana Kolašinac ◽  
Dirk Bier ◽  
Laura Schmitt ◽  
Andriy Yabluchanskiy ◽  
Bernd Neumaier ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Cellular Uptake ◽  
Cancer Cell Line ◽  
Drug Carriers ◽  
Cargo Delivery ◽  
Human Blood Cells ◽  
Fusion Ability ◽  
Potential Use

Liposomes are highly biocompatible and versatile drug carriers with an increasing number of applications in the field of nuclear medicine and diagnostics. So far, only negatively charged liposomes with intercalated radiometals, e.g., 64Cu, 99mTc, have been reported. However, the process of cellular uptake of liposomes by endocytosis is rather slow. Cellular uptake can be accelerated by recently developed cationic liposomes, which exhibit extraordinarily high membrane fusion ability. The aim of the present study was the development of the formulation and the characterization of such cationic fusogenic liposomes with intercalated radioactive [131I]I− for potential use in therapeutic applications. The epithelial human breast cancer cell line MDA-MB-231 was used as a model for invasive cancer cells and cellular uptake of [131I]I− was monitored in vitro. Delivery efficiencies of cationic and neutral liposomes were compared with uptake of free iodide. The best cargo delivery efficiency (~10%) was achieved using cationic fusogenic liposomes due to their special delivery pathway of membrane fusion. Additionally, human blood cells were also incubated with cationic control liposomes and free [131I]I−. In these cases, iodide delivery efficiencies remained below 3%.

scholarly journals Colored Corn: An Up-Date on Metabolites Extraction, Health Implication, and Potential Use

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 199
Author(s):  
Raffaella Colombo ◽  
Lucia Ferron ◽  
Adele Papetti
Keyword(s):  
Metabolic Syndrome ◽  
Plant Secondary Metabolites ◽  
Chemical Characterization ◽  
Carotenoid Composition ◽  
In Vivo Animal Models ◽  
Potential Use ◽  
Blue Corn

Colored (orange, pink, red, purple, and blue) corn strongly attracted attention on its healthy properties mainly due to its anthocyanin and carotenoid composition which is also responsible for its pigmentation. The present review summarized the recent updates on the extraction and chemical characterization of the main plant secondary metabolites present in colored seeds, kernel, cob, husk, and silk. The main approaches used to stabilize the extracts have been discussed as well as their food and non-food uses. Both in vitro and in vivo (animal models) studies on the different effects (antibacterial, antimutagenic, antioxidant, and anti-inflammatory activities, effects on metabolic syndrome, diabetes, glucose and lipidic metabolism, and neuroprotection) of pigmented extracts on animal and human health have been summarized.

scholarly journals In Vitro Cytotoxicity of Aspilia Pluriseta Schweinf. Extract Fractions

2020 ◽  
Author(s):  
Sospeter Ngoci Njeru ◽  
Jackson Mbithi Muema
Keyword(s):  
Biological Activities ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Root Extracts ◽  
Lack Of Information ◽  
Information Gap ◽  
Diphenyltetrazolium Bromide ◽  
Potential Use

Abstract Objectives: We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on A. pluriseta cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemicals characterization of A. pluriseta root extracts, here we report the missing data on cytotoxicity of tested extracts. We evaluated the potential cytotoxicity of the root extracts on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: We show that all solvent extracts (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) of greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivity are within the acceptable cytotoxicity and selective index limits. This scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.

scholarly journals Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1056
Author(s):  
Makoto Shimooka ◽  
Yoshimi Sakurai ◽  
Yasukazu Muramatsu ◽  
Leo Uchida
Keyword(s):  
Epidemiological Data ◽  
Rrna Gene ◽  
Aedes Japonicus ◽  
Vitro Assay ◽  
Isolation And Characterization ◽  
First Case ◽  
Biological Vector ◽  
Potential Use

Species of the genus Spiroplasma are common within arthropods and plants worldwide. Mosquito-associated Spiroplasma spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effects on public health. Although Spiroplasma spp. are expected to have potential use as biological vector-control tools, characteristics such as their distribution, host species, and cytopathogenic effects (CPEs) are not well understood. In this study, we isolated a Spiroplasma sp. from a female Aedes japonicus collected in Hokkaido, northern Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated our isolate was closely related to S. cantharicola. We screened 103 mosquito pools consisting of 3 genera and 9 species, but only detected S. cantharicola in the first isolation. In an in vitro assay, our isolate grew well at 28 °C, but no propagation was observed at 37 °C. Furthermore, the isolate showed strong CPE on a mosquito-derived cultured cell line (C6/36), and its propagation slightly increased when co-cultured with C6/36 cells. To our knowledge, this is the third report of the isolation of S. cantharicola from mosquitoes and the first case in Asia. Our findings provide epidemiological data on S. cantharicola distribution in the region.

The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme

Microbiology ◽  
2005 ◽  
Vol 151 (6) ◽  
pp. 2017-2023 ◽  
Author(s):  
Lei Dong ◽  
Ni Cheng ◽  
Ming-Wei Wang ◽  
Junfeng Zhang ◽  
Chang Shu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Biochemical Characterization ◽  
Gene Encoding ◽  
Allosteric Enzyme ◽  
H Pylori ◽  
Potential Use ◽  
Leucyl Aminopeptidase ◽  
Cobalt And Nickel

This study describes the cloning, genetic analysis and biochemical characterization of a leucyl aminopeptidase (LAP) from Helicobacter pylori. A gene encoding LAP was cloned from H. pylori and the expressed 55 kDa protein displayed homology to aminopeptidases from Gram-negative bacteria, plants and mammals. This LAP demonstrated amidolytic activity against l-leucine-p-nitroanilide. Optimal activity was observed at pH 8·0 and 45 °C, with V max of 232 μmol min−1 (mg protein)−1 and S 0·5 of 0·65 mM. The data suggest that LAP could be allosteric (n H=2·27), with regulatory homohexamers, and its activity was inhibited by ion chelators and enhanced by divalent manganese, cobalt and nickel cations. Bestatin inhibited both LAP activity (IC50=49·9 nM) and H. pylori growth in vitro. The results point to the potential use of LAP as a drug target to develop novel anti-H. pylori agents.

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