scholarly journals Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism

2011 ◽  
Vol 46 (10) ◽  
pp. 827-832 ◽  
Author(s):  
Gabriela Botta ◽  
Christina S. Turn ◽  
Nicholas J. Quintyne ◽  
Paul A. Kirchman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Life Span ◽  
Respiratory Metabolism ◽  
Life Span Extension ◽  
Replicative Life Span

scholarly journals Chronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1

Microbiology ◽  
2007 ◽  
Vol 153 (11) ◽  
pp. 3667-3676 ◽  
Author(s):  
Gemma Reverter-Branchat ◽  
Elisa Cabiscol ◽  
Jordi Tamarit ◽  
M. Alba Sorolla ◽  
M. Ángeles de la Torre ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcohol Dehydrogenase ◽  
Life Span ◽  
Life Span Extension ◽  
Replicative Life Span ◽  
Alcohol Dehydrogenase 1

scholarly journals Loss of Ubp3 increases silencing, decreases unequal recombination in rDNA, and shortens the replicative life span in Saccharomyces cerevisiae

2014 ◽  
Vol 25 (12) ◽  
pp. 1916-1924 ◽  
Author(s):  
David Öling ◽  
Rehan Masoom ◽  
Kristian Kvint
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Life Span ◽  
Rna Polymerase Ii ◽  
Ribosomal Dna ◽  
Genetic Evidence ◽  
Wild Type ◽  
Replicative Life Span ◽  
Unequal Recombination

Ubp3 is a conserved ubiquitin protease that acts as an antisilencing factor in MAT and telomeric regions. Here we show that ubp3∆ mutants also display increased silencing in ribosomal DNA (rDNA). Consistent with this, RNA polymerase II occupancy is lower in cells lacking Ubp3 than in wild-type cells in all heterochromatic regions. Moreover, in a ubp3∆ mutant, unequal recombination in rDNA is highly suppressed. We present genetic evidence that this effect on rDNA recombination, but not silencing, is entirely dependent on the silencing factor Sir2. Further, ubp3∆ sir2∆ mutants age prematurely at the same rate as sir2∆ mutants. Thus our data suggest that recombination negatively influences replicative life span more so than silencing. However, in ubp3∆ mutants, recombination is not a prerequisite for aging, since cells lacking Ubp3 have a shorter life span than isogenic wild-type cells. We discuss the data in view of different models on how silencing and unequal recombination affect replicative life span and the role of Ubp3 in these processes.

Life-span extension by a metacaspase in the yeast Saccharomyces cerevisiae

Science ◽  
2014 ◽  
Vol 344 (6190) ◽  
pp. 1389-1392 ◽  
Author(s):  
S. M. Hill ◽  
X. Hao ◽  
B. Liu ◽  
T. Nystrom
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Life Span ◽  
Yeast Saccharomyces Cerevisiae ◽  
Life Span Extension

scholarly journals Extracted Trans-Resveratrol from Arachis hypogaea Enhances Expression of Sirtuin Gene and Replicative Life Span in Saccharomyces cerevisiae

2020 ◽  
pp. 48-59
Author(s):  
Mritunjay Kumar Singh ◽  
Ravi Deval
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Life Span ◽  
Arachis Hypogaea ◽  
Plant Extract ◽  
Yeast Culture ◽  
Replicative Life Span ◽  
Peanut Plant ◽  
Rp Hplc

Aims: Biotic stress given by Aspergillus niger enhances trans-resveratrol production in Arachis hypogaea plant. This plant extract  increases sir2 gene expression and Replicative Life Span in  Saccharomyces cerevisiae. Design of Study: Peanut plant was grown in aseptic environment, infected by Aspergillus niger. Plant extract used for quantification of trans-resveratrol by RP-HPLC. Yeast culture was grown in Potato dextrose media along with plant extract. Sir2 gene expression fold calculated by real time pcr. Replicative Life Span of yeast was measured by spectrophotometer. Place and Duration of Study: Allele Life Sciences Pvt. Ltd., Department of Biotechnology between February 2017 to March 2020. Methodology: Biotic stress in Arachis hypogaea plant was induced by wounding the leaves and introducing Aspergillus niger to enhance trans-resveratrol production. Tran-resveratrol was quantified by Reverse Phase High Pressure Liquid Chromatography (RP-HPLC). Two methods conducted to check reverse ageing, first one epigenetic based, when extracted trans-resveratrol from infected Arachis hypogaea plant extract added to Saccharomyces cerevisiae culture, it enhanced expression of Sir2 gene in Saccharomyces cerevisiae measured by qPCR, ABI applied biosystem. Process included RNA isolation, cDNA synthesis and thereafter qPCR. Enhanced expression of sirtuin responsible for gene silencing as sirtuin (Sir2 gene product) is a class of Histone deacetylase transferase enzyme. Second method, Replicative Life Span of Saccharomyces cerevisiae culture increased when Aspergillus niger infected peanut plant extract added to yeast culture which was measured through spectrophotometer at 600nm and showed high absorbance value. Results: Tran-resveratrol was quantified by Reverse Phase High Pressure Liquid Chromatography (RP-HPLC) and yield was 2.24 mg/g. Sir2 gene expression increased by 1.56 fold in yeast grown in infected peanut plant extract. Absorbance of yeast culture grown in infected peanut plant extract was 0.522±0.008 which was higher than control. Conclusion: Sir2 gene expression enhances along with replicative life span in yeast in presence of peanut plant extract.

scholarly journals Mitochondria-mediated hormetic response in life span extension of calorie-restricted Saccharomyces cerevisiae

AGE ◽  
2010 ◽  
Vol 33 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Praveen Kumar Sharma ◽  
Vineet Agrawal ◽  
Nilanjan Roy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Life Span ◽  
Life Span Extension ◽  
Hormetic Response

scholarly journals Control of the Replicative Life Span of Human Fibroblasts by p16 and the Polycomb Protein Bmi-1

2003 ◽  
Vol 23 (1) ◽  
pp. 389-401 ◽  
Author(s):  
Koji Itahana ◽  
Ying Zou ◽  
Yoko Itahana ◽  
Jose-Luis Martinez ◽  
Christian Beausejour ◽  
...  
Keyword(s):  
Life Span ◽  
Replicative Senescence ◽  
Human Fibroblasts ◽  
Ring Finger ◽  
Dominant Negative ◽  
Life Span Extension ◽  
Replicative Life Span ◽  
Polycomb Protein ◽  
Human Fibroblast Strains ◽  
Dominant Negative Activity

ABSTRACT The polycomb protein Bmi-1 represses the INK4a locus, which encodes the tumor suppressors p16 and p14ARF. Here we report that Bmi-1 is downregulated when WI-38 human fibroblasts undergo replicative senescence, but not quiescence, and extends replicative life span when overexpressed. Life span extension by Bmi-1 required the pRb, but not p53, tumor suppressor protein. Deletion analysis showed that the RING finger and helix-turn-helix domains of Bmi-1 were required for life span extension and suppression of p16. Furthermore, a RING finger deletion mutant exhibited dominant negative activity, inducing p16 and premature senescence. Interestingly, presenescent cultures of some, but not all, human fibroblasts contained growth-arrested cells expressing high levels of p16 and apparently arrested by a p53- and telomere-independent mechanism. Bmi-1 selectively extended the life span of these cultures. Low O2 concentrations had no effect on p16 levels or life span extension by Bmi-1 but reduced expression of the p53 target, p21. We propose that some human fibroblast strains are more sensitive to stress-induced senescence and have both p16-dependent and p53/telomere-dependent pathways of senescence. Our data suggest that Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.

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