scholarly journals PAX6 regulates human corneal epithelium cell identity

2017 ◽  
Vol 154 ◽  
pp. 30-38 ◽  
Author(s):  
Koji Kitazawa ◽  
Takafusa Hikichi ◽  
Takahiro Nakamura ◽  
Chie Sotozono ◽  
Shigeru Kinoshita ◽  
...  
Keyword(s):  
Corneal Epithelium ◽  
Cell Identity ◽  
Epithelium Cell ◽  
Human Corneal Epithelium

313 Cytotoxicity evaluation of three phthalocyanine photosensitisers on human corneal epithelium cell cultures

2003 ◽  
Vol 144 ◽  
pp. s86
Author(s):  
D. Monti ◽  
S. Burgalassi ◽  
P. Chetoni ◽  
G. Roncucci ◽  
S. Tampucci ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Corneal Epithelium ◽  
Epithelium Cell ◽  
Human Corneal Epithelium

scholarly journals Effects of ambient particulate matter on a reconstructed human corneal epithelium model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryota Ko ◽  
Masahiko Hayashi ◽  
Miho Tanaka ◽  
Tomoaki Okuda ◽  
Chiharu Nishita-Hara ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Cell Viability ◽  
Corneal Epithelium ◽  
Dependent Manner ◽  
Ambient Particulate Matter ◽  
Tissue Sections ◽  
Human Corneal Epithelium ◽  
Vitro Model ◽  
Dose Dependent

AbstractWe evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.

Specular Microscopic Observation of Normal Human Corneal Epithelium

Ophthalmology ◽  
1992 ◽  
Vol 99 (1) ◽  
pp. 89-94 ◽  
Author(s):  
Kazuo Tsubota ◽  
Masakazu Yamada ◽  
Shoji Naoi
Keyword(s):  
Corneal Epithelium ◽  
Microscopic Observation ◽  
Normal Human ◽  
Human Corneal Epithelium

A new 3D reconstituted human corneal epithelium model as an alternative method for the eye irritation test

2011 ◽  
Vol 25 (1) ◽  
pp. 403-410 ◽  
Author(s):  
Kyoung-Mi Jung ◽  
Su-Hyon Lee ◽  
Yang-Hwan Ryu ◽  
Won-Hee Jang ◽  
Haeng-Sun Jung ◽  
...  
Keyword(s):  
Corneal Epithelium ◽  
Eye Irritation ◽  
Alternative Method ◽  
Irritation Test ◽  
Human Corneal Epithelium
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