Involvement of P2X 7 receptors in retinal ganglion cell apoptosis induced by activated Müller cells

2016 ◽  
Vol 153 ◽  
pp. 42-50 ◽  
Author(s):  
Bo Xue ◽  
Yuting Xie ◽  
Ying Xue ◽  
Nan Hu ◽  
Guowei Zhang ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Apoptosis ◽  
Müller Cells ◽  
Retinal Ganglion ◽  
Muller Cells

scholarly journals Activated Müller Cells Involved in ATP-Induced Upregulation of P2X7Receptor Expression and Retinal Ganglion Cell Death

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Xue ◽  
Yuting Xie ◽  
Bo Xue ◽  
Nan Hu ◽  
Guowei Zhang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ganglion Cell ◽  
Intravitreal Injection ◽  
Retinal Ganglion Cell ◽  
Cell Activation ◽  
Müller Cells ◽  
Müller Cell ◽  
Retinal Ganglion ◽  
Muller Cells ◽  
Muller Cell

P2X7receptor (P2X7R), an ATP-gated ion channel, plays an important role in glaucomatous retinal ganglion cell (RGC) apoptotic death, in which activated retinal Müller glial cells may be involved by releasing ATP. In the present study, we investigated whether and how activated Müller cells may induce changes in P2X7R expression in RGCs by using immunohistochemistry and Western blot techniques. Intravitreal injection of DHPG, a group I metabotropic glutamate receptor (mGluR I) agonist, induced upregulation of GFAP expression, suggestive of Müller cell activation (gliosis), as we previously reported. Accompanying Müller cell activation, P2X7R protein expression was upregulated, especially in the cells of ganglion cell layer (GCL), which was reversed by coinjection of brilliant blue G (BBG), a P2X7R blocker. In addition, intravitreal injection of ATP also induced upregulation of P2X7R protein expression. Similar results were observed in cultured retinal neurons by ATP treatment. Moreover, both DHPG and ATP intravitreal injection induced a reduction in the number of fluorogold retrogradely labeled RGCs, and the DHPG effect was partially rescued by coinjection of BBG. All these results suggest that activated Müller cells may release ATP and, in turn, induce upregulation of P2X7R expression in the cells of GCL, thus contributing to RGC death.

scholarly journals Asiatic Acid Prevents Retinal Ganglion Cell Apoptosis in a Rat Model of Glaucoma

2018 ◽  
Vol 12 ◽  
Author(s):  
Wanjing Huang ◽  
Fengjuan Gao ◽  
Fangyuan Hu ◽  
Jiancheng Huang ◽  
Min Wang ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Rat Model ◽  
Cell Apoptosis ◽  
Asiatic Acid ◽  
Retinal Ganglion

In vivo imaging of retinal ganglion cell apoptosis

2013 ◽  
Vol 13 (1) ◽  
pp. 123-127 ◽  
Author(s):  
Joana Galvao ◽  
Benjamin M Davis ◽  
Maria Francesca Cordeiro
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Apoptosis ◽  
In Vivo Imaging ◽  
Retinal Ganglion
Sign in / Sign up

Export Citation Format

Share Document