scholarly journals Method for single illumination source combined optical coherence tomography and fluorescence imaging of fluorescently labeled ocular structures in transgenic mice

2016 ◽  
Vol 151 ◽  
pp. 68-74 ◽  
Author(s):  
Ryan P. McNabb ◽  
Tomas Blanco ◽  
Howard M. Bomze ◽  
Henry C. Tseng ◽  
Daniel R. Saban ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Transgenic Mice ◽  
Fluorescence Imaging ◽  
Optical Coherence ◽  
Illumination Source

scholarly journals Quantitative imaging of cochlear soft tissues in wild-type and hearing-impaired transgenic mice by spectral domain optical coherence tomography

2011 ◽  
Vol 19 (16) ◽  
pp. 15415 ◽  
Author(s):  
Simon S. Gao ◽  
Anping Xia ◽  
Tao Yuan ◽  
Patrick D. Raphael ◽  
Ryan L. Shelton ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Transgenic Mice ◽  
Soft Tissues ◽  
Quantitative Imaging ◽  
Hearing Impaired ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Wild Type

scholarly journals Intravital microscopic optical coherence tomography imaging to assess mucus-mobilizing interventions for muco-obstructive lung disease in mice

2020 ◽  
Vol 318 (3) ◽  
pp. L518-L524 ◽  
Author(s):  
Mario Pieper ◽  
Hinnerk Schulz-Hildebrandt ◽  
Marcus A. Mall ◽  
Gereon Hüttmann ◽  
Peter König
Keyword(s):  
Optical Coherence Tomography ◽  
Transgenic Mice ◽  
Lung Disease ◽  
Hypertonic Saline ◽  
Obstructive Lung Disease ◽  
Lung Diseases ◽  
Isotonic Saline ◽  
Optical Coherence ◽  
Obstructive Lung Diseases

Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma, and COPD, and the development of more effective mucus-mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined whether newly developed high-speed microscopic optical coherence tomography (mOCT) is sensitive to detect and compare in vivo effects of inhaled isotonic saline, hypertonic saline, and bicarbonate on mucus mobilization and clearance in Scnn1b-transgenic mice with muco-obstructive lung disease. In vivo mOCT imaging showed that inhaled isotonic saline-induced rapid mobilization of mucus that was mainly transported as chunks from the lower airways of Scnn1b-transgenic mice. Hypertonic saline mobilized a significantly greater amount of mucus that showed a more uniform distribution compared with isotonic saline. The addition of bicarbonate-to-isotonic saline had no effect on mucus mobilization, but also led to a more uniform mucus layer compared with treatment with isotonic saline alone. mOCT can detect differences in response to mucus-mobilizing interventions in vivo, and may thus support the development of more effective therapies for patients with muco-obstructive lung diseases.

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