Distribution of complement anaphylatoxin receptors and membrane-bound regulators in normal human retina

2006 ◽  
Vol 83 (4) ◽  
pp. 834-840 ◽  
Author(s):  
Susan D. Vogt ◽  
Scott R. Barnum ◽  
Christine A. Curcio ◽  
Russell W. Read
Keyword(s):  
Human Retina ◽  
Anaphylatoxin Receptors ◽  
Normal Human ◽  
Membrane Bound

scholarly journals Amino acid immunoreactivity in normal human retina and after brachytherapy

2013 ◽  
Vol 96 (5) ◽  
pp. 504-507 ◽  
Author(s):  
Clairton F de Souza ◽  
Monica L Acosta ◽  
Philip J Polkinghorne ◽  
Charles NJ McGhee ◽  
Michael Kalloniatis
Keyword(s):  
Amino Acid ◽  
Human Retina ◽  
Normal Human

Macromolecular markers in normal human retina and applications to human retinal disease

2016 ◽  
Vol 150 ◽  
pp. 135-148 ◽  
Author(s):  
Clairton F. de Souza ◽  
Lisa Nivison-Smith ◽  
David L. Christie ◽  
Phillip Polkinghorne ◽  
Charles McGhee ◽  
...  
Keyword(s):  
Retinal Disease ◽  
Human Retina ◽  
Normal Human

Study of normal human retina cell by adaptive optics and pathological way

2005 ◽  
Author(s):  
Chuihui Jiang ◽  
Yanhua Chu ◽  
Wenji Wang ◽  
Gezhi Xu ◽  
Ning Ling ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Human Retina ◽  
Normal Human

scholarly journals Characterization of the interactions between procoagulant albumin and human endothelial cells

Blood ◽  
1993 ◽  
Vol 82 (9) ◽  
pp. 2684-2692
Author(s):  
KJ Faucette ◽  
LA Fitzgerald ◽  
L Liu ◽  
CJ Parker ◽  
GM Rodgers
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Quantitative Polymerase Chain Reaction ◽  
Scavenger Receptor ◽  
Kinase C ◽  
Normal Human ◽  
Membrane Bound ◽  
Polymerase Chain ◽  
Umbilical Vein Endothelial Cells

Normal human plasma contains procoagulant albumin (PC-Al), an anionic form of albumin that induces tissue factor (TF) activity in human umbilical vein endothelial cells (HUVEC) and monocytes. In this study, we investigated both the interactions between HUVEC and PC-Al and the mechanism by which PC-Al induces TF activity. Binding of PC-Al to HUVEC was specific and reversible. Further studies indicated that membrane- bound PC-Al was not internalized by HUVEC. A potential receptor on HUVEC was suggested by studies in which the capacity of a variety of reagents to inhibit the activity of PC-Al was quantitated. Induction of TF activity by PC-Al was antagonized by dextran sulfate, heparin, fucoidan, and concanavalin A but not by ovalbumin, polyglutamic acid, or polyvinyl sulfate. This competition profile bears similarities to those reported for scavenger receptors that have been identified on both HUVEC and monocytes. Involvement of protein kinase C (PKC) in the PC-Al-induced enhancement of TF activity was suggested by experiments in which staurosporine, an inhibitor of PKC, suppressed the activity of PC-Al. The induction of TF activity by PC-Al was further characterized by using a quantitative polymerase chain reaction assay. Increased TF mRNA was first seen after 1 hour of incubation with PC-Al. Maximal observed expression occurred at 2 hours, but at 5 hours, expression had significantly decreased. Monocytes could also be induced to express TF mRNA after a 2-hour incubation with PC-Al. These results suggest that the functionally relevant binding of PC-Al to HUVEC may be mediated through interactions with a membrane constituent that has some of the properties of a scavenger receptor and that this interaction augments TF activity by enhancing transcription of TF mRNA, at least in part, by a mechanism that is dependent on activation of PKC.

scholarly journals Formation of lipid inclusions in normal human leukocytes

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 309-320
Author(s):  
EM Lutas ◽  
D Zucker-Franklin
Keyword(s):  
Room Temperature ◽  
Sucrose Density Gradient ◽  
Peripheral Blood Leukocytes ◽  
Histochemical Analysis ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood ◽  
Lipid Inclusions ◽  
Normal Human ◽  
Membrane Bound ◽  
Layer Chromatography

Normal peripheral blood leukocytes left standing at room temperature develop large inclusions which increase in number and size with time of incubation. The formation and nature of these inclusions were investigated. At 0 hour the structures were present in only 1% of the cells, whereas at 48 hr, they were in virtually all neutrophils and monocytes. Ultrastructurally, the globules measured 0.5–1.5 mu in diameter and they were usually not membrane bound. Histochemical analysis indicated that they were lipid in nature. The inclusions were separated on a sucrose density gradient and their isolation was confirmed by electron microscopy. Thin-layer chromatography of the isolated globules suggested that they consisted predominantly of triglycerides. Since it is known that the cells synthesize triglycerides at rest, it was postulated that the structures may represent the storage form for free fatty acids which may be utilized for membrane synthesis during phagocytosis.

scholarly journals Formation of lipid inclusions in normal human leukocytes

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 309-320 ◽  
Author(s):  
EM Lutas ◽  
D Zucker-Franklin
Keyword(s):  
Room Temperature ◽  
Sucrose Density Gradient ◽  
Peripheral Blood Leukocytes ◽  
Histochemical Analysis ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood ◽  
Lipid Inclusions ◽  
Normal Human ◽  
Membrane Bound ◽  
Layer Chromatography

Abstract Normal peripheral blood leukocytes left standing at room temperature develop large inclusions which increase in number and size with time of incubation. The formation and nature of these inclusions were investigated. At 0 hour the structures were present in only 1% of the cells, whereas at 48 hr, they were in virtually all neutrophils and monocytes. Ultrastructurally, the globules measured 0.5–1.5 mu in diameter and they were usually not membrane bound. Histochemical analysis indicated that they were lipid in nature. The inclusions were separated on a sucrose density gradient and their isolation was confirmed by electron microscopy. Thin-layer chromatography of the isolated globules suggested that they consisted predominantly of triglycerides. Since it is known that the cells synthesize triglycerides at rest, it was postulated that the structures may represent the storage form for free fatty acids which may be utilized for membrane synthesis during phagocytosis.

Demonstration of S-100 Protein Immunoreactivity in Normal Human Retina and Retinoblastoma

1997 ◽  
Vol 211 (6) ◽  
pp. 351-353 ◽  
Author(s):  
Mohammed Mohibul Karim ◽  
Hiroshi Itoh
Keyword(s):  
Human Retina ◽  
S 100 ◽  
Normal Human ◽  
Protein Immunoreactivity
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