scholarly journals Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome

2016 ◽  
Vol 10 ◽  
pp. 28-30 ◽  
Author(s):  
Simona Nonnis ◽  
Elisa Maffioli ◽  
Lucia Zanotti ◽  
Fabiana Santagata ◽  
Armando Negri ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Ms Analysis ◽  
Fetal Bovine

scholarly journals Human olfactory mesenchymal stromal cells co-expressing horizontal basal and ensheathing cell proteins in culture

Biomédica ◽  
2020 ◽  
Vol 40 (1) ◽  
pp. 72-88
Author(s):  
Carlos Ayala-Grosso ◽  
Rosalinda Pieruzzini ◽  
Leslie Vargas-Saturno ◽  
José E. Cardier
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Olfactory Mucosa ◽  
Serum Free Medium ◽  
Free Medium ◽  
Phenotypic Markers ◽  
Serum Free ◽  
Fetal Bovine

Introduction: The olfactory neuro-epithelium has an intrinsic capability of renewal during lifetime provided by the existence of globose and horizontal olfactory precursor cells. Additionally, mesenchymal stromal olfactory cells also support the homeostasis of the olfactory mucosa cell population. Under in vitro culture conditions with Dulbecco modified eagle/F12 medium supplemented with 10% fetal bovine serum, tissue biopsies from upper turbinate have generated an adherent population of cells expressing mainly mesenchymal stromal phenotypic markers. A closer examination of these cells has also found co-expression of olfactory precursors and ensheathing cell phenotypic markers. These results were suggestive of a unique property of olfactory mesenchymal stromal cells as potentially olfactory progenitor cells.Objective: To study whether the expression of these proteins in mesenchymal stromal cells is modulated upon neuronal differentiation.Materials and methods: We observed the phenotype of olfactory stromal cells under DMEM/F12 plus 10% fetal bovine serum in comparison to cells from spheres induced by serum-free medium plus growth factors inducers of neural progenitors.Results: The expression of mesenchymal stromal (CD29+, CD73+, CD90+, CD45-), horizontal basal (ICAM-1/CD54+, p63+, p75NGFr+), and ensheathing progenitor cell (nestin+, GFAP+) proteins was determined in the cultured population by flow cytometry. The determination of Oct 3/4, Sox-2, and Mash-1 transcription factors, as well as the neurotrophins BDNF, NT3, and NT4 by RT-PCR in cells, was indicative of functional heterogeneity of the olfactory mucosa tissue sample. Conclusions: Mesenchymal and olfactory precursor proteins were downregulated by serum-free medium and promoted differentiation of mesenchymal stromal cells into neurons and astroglial cells.

Stimulatory Effects of Serum for Ex-Vivo Culture of Mesenchymal Stromal Cells Can be a Parameter for Heterogeneity in Hematopoietic Supporting Activity

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 5407-5407
Author(s):  
Jung-Ho Kim ◽  
Ho-Sun Lee ◽  
In-Sun Chu ◽  
Il-Hoan Oh
Keyword(s):  
Clinical Trials ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Conflicts Of Interest ◽  
Fetal Bovine Serum ◽  
Ex Vivo ◽  
Significant Heterogeneity ◽  
Fetal Bovine ◽  
Ex Vivo Culture

Abstract Although hematopoietic supporting activity of mesenchymal stromal cells (MSCs) have been widely used in clinical trials to facilitate the hematopoietic recovery, variations have been observedin supporting effects. In the present study, we show that the niche activity of MSCs is regulated by stimulatory effects of fetal bovine serum during ex-vivo culture of MSCs.First, we observed extensive variations in the numbers of colonies (CFU-F)(up to 40-folds) differences in numbers when human bone marrow (BM)-derived MSCs were cultured under different batches of fetal bovine serum (FBS),and two batches with high (SS) and low (NSS) stimulating effects were selected. 5 independent BM-MSCs cultured with stimulating serum exhibitedsignificantly lower doubling times, higher numbers of CFU-F and higher % of CD146+ cells. When umbilical cord blood-derived CD34+ cells were co-cultured on each MSC group, significantly higher expansion of CD34+CD90+ cells and higher numbers of colonies were observed than those cultured with non-stimulatory serum. Similarly, numbers of primitive hematopoietic cells analyzed by long-term cultureinitiating cells (LTC-IC) were significantly higher in stimulating serum conditions than in non-stimulating condition. Furthermore, MSCs under stimulatory serum exhibited higher level expression of niche cross-talk molecules (CXCL-12 and Jagged-1) than MSCs under non-stimulatory condition, indicating higher support of HSC self-renewal. Whenexamined for murine model, the stimulatory serum caused similar difference in colonogenic activity (2.5-folds higher CFU-F) and phenotypic marker for mesenchymal progenitors (PDGFR-alpha+ cells). Moreover, when each group MSCs were co-transplanted with HSCs into congenic recipient, significantly higher reconstitution of donor-derived cells were observed without changes in the lympho-myeloid lineage distribution in the MSCs with stimulatory serum group than in MSCs with non-stimulatory group (51.2% vs 8.2%, respectively, p<0.005). In contrast, priming of HSCs with 2hr pre-incubation with each group MSCs before transplantation or co-transplantation of MSCs cultured under hypoxic conditions did not exhibit additional increase in the reconstitution level by donor-derived cells. Of note, the enhancing effects of stimulatory serum on MSCs or on the co-cultured HSCs were reversed when the MSC culture conditions were switched to non-stimulatory serum, indicating that the enhancing effects were caused by extrinsic stimulating effects rather than clonal difference of MSC subpopulation. To explore the differential signals between MSCs grown under stimulatory and non-stimulatory condition, microarray-based analysis of gene expression were analyzed. The gene ontology (GO) analysis showed that extensive signals related to cell growth and survival was enriched in the MSCs grown in stimulatory conditions compared to those in non-stimulatory conditions. Furthermore, upstream signal analysis showed that signals for P53, TRIB3, TGF-beta1, RABL6 or ATF4 were significantly up-regulated in the MSCs under stimulatory serum condition. Taken together, our study shows that MSCs can exhibit significant heterogeneity in the hematopoietic niche activity depending on the stimulatory effects of the serum during ex-vivo culture to cause difference in hematological recovery, and suggest that optimization of CFU-F-based screening of MSCs can produce more predictable outcomes in clinical trials. Disclosures No relevant conflicts of interest to declare.

Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells

Transfusion ◽  
2007 ◽  
Vol 47 (8) ◽  
pp. 1436-1446 ◽  
Author(s):  
Katharina Schallmoser ◽  
Christina Bartmann ◽  
Eva Rohde ◽  
Andreas Reinisch ◽  
Karl Kashofer ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Clinical Scale ◽  
Human Platelet Lysate ◽  
Fetal Bovine ◽  
Scale Expansion

scholarly journals Human Alternatives to Fetal Bovine Serum for the Expansion of Mesenchymal Stromal Cells from Bone Marrow

Stem Cells ◽  
2009 ◽  
Vol 27 (9) ◽  
pp. 2331-2341 ◽  
Author(s):  
Karen Bieback ◽  
Andrea Hecker ◽  
Asli Kocaömer ◽  
Heinrich Lannert ◽  
Katharina Schallmoser ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cells

2012 ◽  
Vol 34 (7) ◽  
pp. 1367-1374 ◽  
Author(s):  
Sanjay Gottipamula ◽  
Archana Sharma ◽  
Sagar Krishnamurthy ◽  
Anish Sen Majumdar ◽  
Raviraja N. Seetharam
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Large Scale ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine
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