Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

2007 ◽  
Vol 58 (4) ◽  
pp. 263-273 ◽  
Author(s):  
Alena Jiroutova ◽  
Rastislav Slavkovsky ◽  
Martina Cermakova ◽  
Lenka Majdiakova ◽  
Irena Hanovcova ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

scholarly journals BIOCHEMICAL MARKERS OF CONNECTIVE TISSUE METABOLISM IN RATS’ BLOOD SERUM WITH EXPERIMENTAL KNEE OSTEOARTHRITIS AND THEIR DYNAMICS DURING CONSERVATIVE TREATMENT

2019 ◽  
Vol 72 (2) ◽  
pp. 193-197
Author(s):  
Kyrylo V. Makolinets ◽  
Vasyl I. Makolinets ◽  
Dmytro V. Morozenko ◽  
Kateryna V. Gliebova ◽  
Svitlana I. Danylchenko
Keyword(s):  
Laser Radiation ◽  
Conservative Treatment ◽  
Connective Tissue ◽  
Biochemical Markers ◽  
Diclofenac Sodium ◽  
Infrared Laser ◽  
Knee Joints ◽  
Glucosamine Hydrochloride ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

Introduction: There are many methods for diagnosis and treatment of knee joints osteoarthritis in modern orthopedics. The infrared radiation is one of the most popular and effective methods though it needs experimental substantiation on rats. The aim: Experimental definition of the effectiveness of the proposed method of conservative treatment of knee joints osteoarthritis using sodium diclofenac, glucosamine hydrochloride and infrared laser radiation, on the basis of biochemical markers of connective tissue metabolism in rats. Materials and methods: Experimental studies were performed on 40 white male rats. A part of rats received infrared laser radiation in addition to diclofenac sodium and glucosamine hydrochloride. Results: Using a complex of infrared laser radiation and glucosamine hydrochloride and diclofenac sodium in conservative treatment of rats with experimentally induced knee joints osteoarthritis rats after 24 days of treatment. Treatment with the above-mentioned scheme resulted in a significant reduction in the content of glycoproteins (28.5%), chondroitin sulfates (26.1%), alkaline phosphatase activity (32.3%), and I and II fractions of glycosaminoglycans, namely chondroitin-6- (28.7%) and chondroitin-4-sulfate (40.3%). Moreover, these indicators of the studied markers approached the level of intact animals. Conclusions: Changes in biochemical markers of connective tissue in rats during experimental knee joints osteoarthritis indicated a higher efficiency of the treatment regimen with the use of low-intensity infrared laser radiation compared with exclusively drug therapy.

scholarly journals Role of lysyl oxidase like 1 in regulation of postpartum connective tissue metabolism in the mouse vagina†

2019 ◽  
Vol 101 (5) ◽  
pp. 916-927 ◽  
Author(s):  
Ali Borazjani ◽  
Bruna M Couri ◽  
Mei Kuang ◽  
Brian M Balog ◽  
Margot S Damaser
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Connective Tissue ◽  
Lysyl Oxidase ◽  
Gelatin Zymography ◽  
Pelvic Organ ◽  
Tissue Metabolism ◽  
Reverse Transcription Pcr ◽  
Connective Tissue Metabolism

Abstract Pelvic organ prolapse (POP) in lysyl oxidase like-1 knockout (Loxl1 KO) mice occurs primarily in parous mice and is rare in nulliparous mice. We determined the effect of Loxl1 deficiency on postpartum regulation of connective tissue metabolism genes and degradative enzyme activity in the vagina at 20 days gestation or 4 h, 48 h, 7 days, 15 days, 25 days, 7 weeks, or 12 weeks postpartum. Nulliparous Loxl1 KO and wildtype (WT) mice aged 11, 18, or 23 weeks were controls. Gene expression and enzyme activity were assessed using real-time quantitative reverse transcription PCR and fluorescein conjugated gelatin zymography, respectively. Parity, but not aging, had a significant influence on gene expression both with time postpartum and between KO and WT mice. Mmp2, Timp1, Timp2, Timp3, Timp4, Col1a1, Col3a1, Acta2, and Bmp1 were differentially expressed between KO and WT mice. Correlational analysis of gene-gene pairs revealed 10 significant differences between parous KO and WT groups, 5 of which were due to lack of co-expression of Bmp1 in KO mice. The overall enzyme activity that could be attributed to MMPs was significantly higher in WT compared to KO mice both 25 days and 12 weeks postpartum, and MMP activity was significantly lower 15 days and 25 days postpartum compared to KO nulliparous controls, but not WT. These findings suggest that Loxl1 deficiency combined with parity has a significant impact on postpartum regulation of connective tissue metabolism, particularly as it relates to co-expression of Bmp1 and altered proteolytic activity.

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