Development of an in vitro test battery for the screening of the receptor-mediated mechanism and the spindle-poison mode of action of estrogenic compounds

2016 ◽  
Vol 48 ◽  
pp. 245-252
Author(s):  
Corinne Quadalti ◽  
Cesare Galli ◽  
Giovanna Lazzari
Keyword(s):  
Mode Of Action ◽  
Test Battery ◽  
In Vitro Test ◽  
Estrogenic Compounds ◽  
Vitro Test

Evaluation of an alternative in vitro test battery for detecting reproductive toxicants

2014 ◽  
Vol 48 ◽  
pp. 11
Author(s):  
A.H. Piersma ◽  
S. Bosgra ◽  
M.B.M. van Duursen ◽  
S.A.B. Hermsen ◽  
L.R.A. Jonker ◽  
...  
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Vitro Test ◽  
Reproductive Toxicants

Development of an in vitro test battery for assessing chemical effects on bovine germ cells under the ReProTect umbrella

2008 ◽  
Vol 233 (3) ◽  
pp. 360-370 ◽  
Author(s):  
Giovanna Lazzari ◽  
Irene Tessaro ◽  
Gabriella Crotti ◽  
Cesare Galli ◽  
Sebastian Hoffmann ◽  
...  
Keyword(s):  
Germ Cells ◽  
Test Battery ◽  
In Vitro Test ◽  
Chemical Effects ◽  
Vitro Test

Use of an in vitro test battery as a prescreen in the assessment of ocular irritancy

1994 ◽  
Vol 8 (1) ◽  
pp. 75-79 ◽  
Author(s):  
R.W. Lewis ◽  
J.C. McCall ◽  
P.A. Botham
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Vitro Test

The genotoxic activity of glycerol in an in vitro test battery

1988 ◽  
Vol 26 (7) ◽  
pp. 631-635 ◽  
Author(s):  
D.J. Doolittle ◽  
D.A. Lee ◽  
C.K. Lee
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Genotoxic Activity ◽  
Vitro Test

The Prediction of Human Acute Systemic Toxicity by the EDIT/MEIC In Vitro Test Battery: The Importance of Protein Binding and of Partitioning into Lipids

2003 ◽  
Vol 31 (3) ◽  
pp. 245-256 ◽  
Author(s):  
Cecilia Clemedson ◽  
Paul J. Dierickx ◽  
Michael Sjöström
Keyword(s):  
Protein Binding ◽  
Mercuric Chloride ◽  
Culture Medium ◽  
Systemic Toxicity ◽  
Test Battery ◽  
In Vitro Test ◽  
Blood Concentrations ◽  
Uptake Inhibition ◽  
Vitro Test

The aim of the two studies presented in this paper was to further improve the predictability of the original Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) in vitro test battery for acute systemic toxicity. In the first study, whether a protein-free cytotoxicity assay could improve the prediction of human acute systemic toxicity was investigated. The cytotoxicity of 39 MEIC reference chemicals was measured by the neutral red uptake inhibition test after 30 minutes in phosphate-buffered saline (PBS), with hepatoma-derived Fa32 cells. The results were compared with the corresponding values obtained in complete culture medium, including 10% fetal calf serum. Mercuric chloride and hexachlorophene were much more cytotoxic in PBS, as was the case, to a lesser extent, for seven other chemicals. Potassium cyanide and eight other chemicals were less cytotoxic in PBS than in complete culture medium, probably because of poor physiological conditions. The correlation between the cytotoxicity measured in PBS and human acute toxicity was rather low, but became of the same order as for other assays, when mercuric chloride and hexachlorophene were withdrawn from the comparison. In the second study, modelling of human lethal blood concentrations by using the results of the three cell line tests of the original MEIC test battery were complemented by logP (octanol–water partition coefficient) values. The introduction of logP into the modelling did not improve the correlations, but some improvement of both R2 and Q2 was obtained by expanding the logP values with logP2 values. The highest R2 (0.84) and Q2 (0.80) values were obtained for a model in which both experimental and calculated (ambiguous) logP values were used. When only experimental logP values were used, the corresponding values were 0.80 and 0.78. These two studies showed that including protein binding and the partition of chemicals in the MEIC in vitro test battery is important, in order to improve the predictability of the results obtained.

Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context

2015 ◽  
Vol 55 ◽  
pp. 11-19 ◽  
Author(s):  
E. Dinant Kroese ◽  
Sieto Bosgra ◽  
Harrie E. Buist ◽  
Geertje Lewin ◽  
Sander C. van der Linden ◽  
...  
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Vitro Test ◽  
Reproductive Toxicants

In vitro test battery to predict skin sensitizers based on key events of sensitization

2012 ◽  
Vol 211 ◽  
pp. S105
Author(s):  
Tzutzuy Ramirez ◽  
Caroline Bauch ◽  
Tobias Eltze ◽  
Susanne N. Kolle ◽  
Annette Mehling ◽  
...  
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Vitro Test ◽  
Key Events

Evaluation of an alternative in vitro test battery for detecting reproductive toxicants

2013 ◽  
Vol 38 ◽  
pp. 53-64 ◽  
Author(s):  
A.H. Piersma ◽  
S. Bosgra ◽  
M.B.M. van Duursen ◽  
S.A.B. Hermsen ◽  
L.R.A. Jonker ◽  
...  
Keyword(s):  
Test Battery ◽  
In Vitro Test ◽  
Vitro Test ◽  
Reproductive Toxicants
Sign in / Sign up

Export Citation Format

Share Document